TY - JOUR
T1 - Interventions targeted at oxidatively generated modifications of nucleic acids focused on urine and plasma markers
AU - Larsen, Emil List
AU - Weimann, Allan
AU - Poulsen, Henrik Enghusen
PY - 2019
Y1 - 2019
N2 - Oxidative stress is associated with the development and progression of numerous diseases. However, targeting oxidative stress has not been established in the clinical management of any disease. Several methods and markers are available to measure oxidative stress, including direct measurement of free radicals, antioxidants, redox balance, and oxidative modifications of cellular macromolecules. Oxidatively generated nucleic acid modifications have attracted much interest due to the pre-mutagenic oxidative modification of DNA into 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG), associated with cancer development. During the last decade, the perception of RNA has changed from that of a ‘silent messenger’ to an ‘active contributor’, and, parallelly oxidatively generated RNA modifications measured as 8-oxo-7,8-dihydro-guanosine (8-oxoGuo), has been demonstrated as a prognostic factor for all-caused and cardiovascular related mortality in patients with type 2 diabetes. Several attempts have been made to modify the amount of oxidative nucleic acid modifications. Thus, this review aims to introduce researchers to the measurement of oxidatively generated nucleic acid modifications as well as critically review previous attempts and provide future directions for targeting oxidatively generated nucleic acid modifications.
AB - Oxidative stress is associated with the development and progression of numerous diseases. However, targeting oxidative stress has not been established in the clinical management of any disease. Several methods and markers are available to measure oxidative stress, including direct measurement of free radicals, antioxidants, redox balance, and oxidative modifications of cellular macromolecules. Oxidatively generated nucleic acid modifications have attracted much interest due to the pre-mutagenic oxidative modification of DNA into 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG), associated with cancer development. During the last decade, the perception of RNA has changed from that of a ‘silent messenger’ to an ‘active contributor’, and, parallelly oxidatively generated RNA modifications measured as 8-oxo-7,8-dihydro-guanosine (8-oxoGuo), has been demonstrated as a prognostic factor for all-caused and cardiovascular related mortality in patients with type 2 diabetes. Several attempts have been made to modify the amount of oxidative nucleic acid modifications. Thus, this review aims to introduce researchers to the measurement of oxidatively generated nucleic acid modifications as well as critically review previous attempts and provide future directions for targeting oxidatively generated nucleic acid modifications.
KW - 8-oxo-7,8-dihydro-2′-deoxyguanosine
KW - 8-oxo-7,8-dihydro-guanosine
KW - Clinical trials
KW - Intervention
KW - Oxidative nucleic acid modifications
KW - Oxidative stress
U2 - 10.1016/j.freeradbiomed.2019.09.030
DO - 10.1016/j.freeradbiomed.2019.09.030
M3 - Journal article
C2 - 31563634
AN - SCOPUS:85072804227
SN - 0891-5849
VL - 145
SP - 256
EP - 283
JO - Free Radical Biology & Medicine
JF - Free Radical Biology & Medicine
ER -
