TY - JOUR
T1 - Interspecific competition study between Pseudochattonella farcimen and P. verruculosa (Dictyochophyceae) - two ichthyotoxic species that co-occur in Scandinavian waters.
AU - Eckford-Soper, Lisa Kathleen
AU - Daugbjerg, Niels
PY - 2017/2/1
Y1 - 2017/2/1
N2 - The genus Pseudochattonella has become a frequent component of late winter-early spring phytoplankton community in Scandinavian waters, causing extensive fish kills and substantial economic losses. One of currently two recognised species, P. farcimen, is often abundant prior to the diatom spring bloom. Recent field studies have revealed that P. farcimen and P. verruculosa have a period of overlap in their temperature ranges and thus their seasonal occurrences. Using laboratory cultures, we investigated the seasonal succession and growth of P. farcimen and P. verruculosa in both mono- and mixed-culture using the recently developed Pseudochattonella ‘qPCR subtraction method’, which for the first time allowed the simultaneous enumeration of these morphologically indistinguishable species in mixed assemblages. We examined how these species interacted over four different temperatures (5, 8, 11 and 15 °C). The observed growth rates and cell yields varied with temperature revealing their preferred temperature optima. P. farcimen was able to achieve positive net growth over all temperatures, while P. verruculosa failed to grow below 11 °C. Growth responses were statistically different between mono- and mixed-cultures with the outcome of these interactions being temperature-dependent. Nutrients (nitrate and phosphate) and pH levels were also measured throughout the growth experiments to better understand how these factors influenced growth of both species. P. verruculosa was shown to be less sensitive to high pH as growth ceased at pH 9.1, whereas P. farcimen stopped growing at pH 8.4. Understanding the influence of abiotic factors (e.g. temperature, pH and competition) on growth rates allows for a better understanding and prediction of phytoplankton community dynamics.
AB - The genus Pseudochattonella has become a frequent component of late winter-early spring phytoplankton community in Scandinavian waters, causing extensive fish kills and substantial economic losses. One of currently two recognised species, P. farcimen, is often abundant prior to the diatom spring bloom. Recent field studies have revealed that P. farcimen and P. verruculosa have a period of overlap in their temperature ranges and thus their seasonal occurrences. Using laboratory cultures, we investigated the seasonal succession and growth of P. farcimen and P. verruculosa in both mono- and mixed-culture using the recently developed Pseudochattonella ‘qPCR subtraction method’, which for the first time allowed the simultaneous enumeration of these morphologically indistinguishable species in mixed assemblages. We examined how these species interacted over four different temperatures (5, 8, 11 and 15 °C). The observed growth rates and cell yields varied with temperature revealing their preferred temperature optima. P. farcimen was able to achieve positive net growth over all temperatures, while P. verruculosa failed to grow below 11 °C. Growth responses were statistically different between mono- and mixed-cultures with the outcome of these interactions being temperature-dependent. Nutrients (nitrate and phosphate) and pH levels were also measured throughout the growth experiments to better understand how these factors influenced growth of both species. P. verruculosa was shown to be less sensitive to high pH as growth ceased at pH 9.1, whereas P. farcimen stopped growing at pH 8.4. Understanding the influence of abiotic factors (e.g. temperature, pH and competition) on growth rates allows for a better understanding and prediction of phytoplankton community dynamics.
U2 - 10.1007/s00248-016-0856-z
DO - 10.1007/s00248-016-0856-z
M3 - Journal article
C2 - 27645137
SN - 0095-3628
VL - 73
SP - 259
EP - 270
JO - Microbial Ecology
JF - Microbial Ecology
IS - 2
ER -