Interferon-¿ production by human T cells and natural killer cells in vitro in response to antigens from the two intracellular pathogens Mycobacterium tuberculosis and Leishmania major

TY - JOUR

T1 - Interferon-¿ production by human T cells and natural killer cells in vitro in response to antigens from the two intracellular pathogens Mycobacterium tuberculosis and Leishmania major

AU - Kemp, K

AU - Hviid, L

AU - Kharazmi, A

AU - Kemp, M

N1 - Keywords: Adult; Animals; Antigens, Bacterial; Antigens, CD3; Antigens, CD56; Antigens, Protozoan; BCG Vaccine; Cells, Cultured; Humans; Immunization; Interferon Type II; Killer Cells, Natural; Leishmania major; Lymphocyte Activation; Mycobacterium tuberculosis; T-Lymphocytes; Th1 Cells; Tuberculin

PY - 1997

Y1 - 1997

N2 - Acquired resistance to both mycobacteria and Leishmania is primarily mediated by interferon-gamma (IFN-gamma), which triggers mechanisms leading to the death of the microorganism in macrophages. In this study, cell activation and IFN-gamma production was investigated in human peripheral blood mononuclear cells (PBMC) from individuals previously sensitized to tuberculin and without known exposure to Leishmania parasites. Immune staining for intracellular IFN-gamma and surface markers allowed flow cytometric identification of the cellular sources of IFN-gamma in cell cultures incubated with purified protein derivative of tuberculin (PPD) and Leishmania antigens. It was found that IFN-gamma was produced in response to both PPD and Leishmania stimulant by T cells in the cultures. Activation of IFN-gamma producing natural killer (NK) cells was demonstrated only in some cultures, and only with concomitant T cell activation.

AB - Acquired resistance to both mycobacteria and Leishmania is primarily mediated by interferon-gamma (IFN-gamma), which triggers mechanisms leading to the death of the microorganism in macrophages. In this study, cell activation and IFN-gamma production was investigated in human peripheral blood mononuclear cells (PBMC) from individuals previously sensitized to tuberculin and without known exposure to Leishmania parasites. Immune staining for intracellular IFN-gamma and surface markers allowed flow cytometric identification of the cellular sources of IFN-gamma in cell cultures incubated with purified protein derivative of tuberculin (PPD) and Leishmania antigens. It was found that IFN-gamma was produced in response to both PPD and Leishmania stimulant by T cells in the cultures. Activation of IFN-gamma producing natural killer (NK) cells was demonstrated only in some cultures, and only with concomitant T cell activation.

U2 - 10.1046/j.1365-3083.1997.d01-154.x

DO - 10.1046/j.1365-3083.1997.d01-154.x

M3 - Journal article

C2 - 9393632

SN - 0301-6323

VL - 46

SP - 495

EP - 499

JO - Scandinavian Journal of Immunology, Supplement

JF - Scandinavian Journal of Immunology, Supplement

IS - 5

ER -