Integrative analyses reveal novel strategies in HPV11,-16 and -45 early infection

TY - JOUR

T1 - Integrative analyses reveal novel strategies in HPV11,-16 and -45 early infection

AU - Kaczkowski, Bogumil

AU - Rossing, Maria

AU - Andersen, Ditte K

AU - Dreher, Anita

AU - Morevati, Marya

AU - Visser, Melissa A

AU - Winther, Ole

AU - Nielsen, Finn Cilius

AU - Norrild, Bodil

PY - 2012

Y1 - 2012

N2 - The interaction between human papillomavirus (HPV) and host cells is not well understood. We investigate the early stage of HPV infections by global expression profiling in a cell model, in which HaCaT cells were transfected with HPV11, HPV16 or HPV45 genomes. We report the differential expression of genes not previously implicated in HPV biology, such as the PSG family and ANKRD1, and of genes implicated in the biology of other viruses, e.g. MX1, IFI44 and DDX60. Carcinogenesis-related genes, e.g. ABL2, MGLL and CYR61, were upregulated by high-risk HPV16 and -45. The integrative analysis revealed the suppression of DNA repair by HPV11 and -16, and downregulation of cytoskeleton genes by all HPV types. Various signalling pathways were affected by the HPVs: IL-2 by HPV11; JAK-STAT by HPV16; and TGF-Î 2, NOTCH and tyrosine kinase signalling by HPV45. This study uncovered novel strategies employed by HPV to establish infection and promote uncontrolled growth.

AB - The interaction between human papillomavirus (HPV) and host cells is not well understood. We investigate the early stage of HPV infections by global expression profiling in a cell model, in which HaCaT cells were transfected with HPV11, HPV16 or HPV45 genomes. We report the differential expression of genes not previously implicated in HPV biology, such as the PSG family and ANKRD1, and of genes implicated in the biology of other viruses, e.g. MX1, IFI44 and DDX60. Carcinogenesis-related genes, e.g. ABL2, MGLL and CYR61, were upregulated by high-risk HPV16 and -45. The integrative analysis revealed the suppression of DNA repair by HPV11 and -16, and downregulation of cytoskeleton genes by all HPV types. Various signalling pathways were affected by the HPVs: IL-2 by HPV11; JAK-STAT by HPV16; and TGF-Î 2, NOTCH and tyrosine kinase signalling by HPV45. This study uncovered novel strategies employed by HPV to establish infection and promote uncontrolled growth.

U2 - 10.1038/srep00515

DO - 10.1038/srep00515

M3 - Journal article

C2 - 22808421

SN - 2045-2322

VL - 2

SP - 515

JO - Scientific Reports

JF - Scientific Reports

ER -