Inositol trisphosphate and thapsigargin discriminate endoplasmic reticulum stores of calcium in rat brain

A Verma; D J Hirsch; M R Hanley; Ole Thastrup; Søren Brøgger Christensen; S H Snyder

Inositol trisphosphate and thapsigargin discriminate endoplasmic reticulum stores of calcium in rat brain

A Verma, D J Hirsch, M R Hanley, Ole Thastrup, Søren Brøgger Christensen, S H Snyder

71 Citationer (Scopus)

Abstract

ATP dependent Ca2+ accumulation into oxalate-loaded rat brain microsomes is potently inhibited by thapsigargin with an IC50 of 2 nM and maximal inhibition at 10 nM. Approximately 15% of the total A23187-releasable microsomal calcium store is insensitive to thapsigargin concentrations up to 100 microM. Inositol-1,4,5-trisphosphate (IP3) maximally inhibits 40% of the net Ca2+ accumulation by whole brain microsomes. Its effects are non-additive with thapsigargin suggesting that the IP3-sensitive Ca2+ pool is a subset of the thapsigargin sensitive Ca2+ pool. Marked regional differences occur in Ca2+ transport rates and sensitivity to both thapsigargin and IP3.

Originalsprog	Engelsk
Tidsskrift	Biochemical and Biophysical Research Communications
Vol/bind	172
Udgave nummer	2
Sider (fra-til)	811-6
Antal sider	6
ISSN	0006-291X
Status	Udgivet - 1990

Citationsformater

@article{200e8e8b504d41048f674b02f8626638,

title = "Inositol trisphosphate and thapsigargin discriminate endoplasmic reticulum stores of calcium in rat brain",

abstract = "ATP dependent Ca2+ accumulation into oxalate-loaded rat brain microsomes is potently inhibited by thapsigargin with an IC50 of 2 nM and maximal inhibition at 10 nM. Approximately 15% of the total A23187-releasable microsomal calcium store is insensitive to thapsigargin concentrations up to 100 microM. Inositol-1,4,5-trisphosphate (IP3) maximally inhibits 40% of the net Ca2+ accumulation by whole brain microsomes. Its effects are non-additive with thapsigargin suggesting that the IP3-sensitive Ca2+ pool is a subset of the thapsigargin sensitive Ca2+ pool. Marked regional differences occur in Ca2+ transport rates and sensitivity to both thapsigargin and IP3.",

keywords = "Animals, Brain, Calcimycin, Calcium, Carcinogens, Endoplasmic Reticulum, Heparin, Inositol 1,4,5-Trisphosphate, Kinetics, Microsomes, Organ Specificity, Rats, Terpenes, Thapsigargin",

author = "A Verma and Hirsch, {D J} and Hanley, {M R} and Ole Thastrup and Christensen, {S{\o}ren Br{\o}gger} and Snyder, {S H}",

year = "1990",

language = "English",

volume = "172",

pages = "811--6",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - Inositol trisphosphate and thapsigargin discriminate endoplasmic reticulum stores of calcium in rat brain

AU - Verma, A

AU - Hirsch, D J

AU - Hanley, M R

AU - Thastrup, Ole

AU - Christensen, Søren Brøgger

AU - Snyder, S H

PY - 1990

Y1 - 1990

N2 - ATP dependent Ca2+ accumulation into oxalate-loaded rat brain microsomes is potently inhibited by thapsigargin with an IC50 of 2 nM and maximal inhibition at 10 nM. Approximately 15% of the total A23187-releasable microsomal calcium store is insensitive to thapsigargin concentrations up to 100 microM. Inositol-1,4,5-trisphosphate (IP3) maximally inhibits 40% of the net Ca2+ accumulation by whole brain microsomes. Its effects are non-additive with thapsigargin suggesting that the IP3-sensitive Ca2+ pool is a subset of the thapsigargin sensitive Ca2+ pool. Marked regional differences occur in Ca2+ transport rates and sensitivity to both thapsigargin and IP3.

AB - ATP dependent Ca2+ accumulation into oxalate-loaded rat brain microsomes is potently inhibited by thapsigargin with an IC50 of 2 nM and maximal inhibition at 10 nM. Approximately 15% of the total A23187-releasable microsomal calcium store is insensitive to thapsigargin concentrations up to 100 microM. Inositol-1,4,5-trisphosphate (IP3) maximally inhibits 40% of the net Ca2+ accumulation by whole brain microsomes. Its effects are non-additive with thapsigargin suggesting that the IP3-sensitive Ca2+ pool is a subset of the thapsigargin sensitive Ca2+ pool. Marked regional differences occur in Ca2+ transport rates and sensitivity to both thapsigargin and IP3.

KW - Animals

KW - Brain

KW - Calcimycin

KW - Calcium

KW - Carcinogens

KW - Endoplasmic Reticulum

KW - Heparin

KW - Inositol 1,4,5-Trisphosphate

KW - Kinetics

KW - Microsomes

KW - Organ Specificity

KW - Rats

KW - Terpenes

KW - Thapsigargin

M3 - Journal article

C2 - 2241971

SN - 0006-291X

VL - 172

SP - 811

EP - 816

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Inositol trisphosphate and thapsigargin discriminate endoplasmic reticulum stores of calcium in rat brain

Abstract

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