Inhibition by indomethacin and aspirin of 15 hydroxy prostaglandin dehydrogenase in vitro

TY - JOUR

T1 - Inhibition by indomethacin and aspirin of 15 hydroxy prostaglandin dehydrogenase in vitro

AU - Hansen, Harald S.

PY - 1974/1/1

Y1 - 1974/1/1

N2 - 15 Hydroxyprostaglandin dehydrogenase from bovine lung was purified 7.4 times to a specific activity of 1.4 mU/mg of protein. The isoelectric point was estimated at 5.4 and the molecular weight by gel filtration at 40,000. K(m) for prostaglandin E and for NAD was found to be 3.4 µM and 1.1 x 10M respectively. The enzyme was inhibited by indomethacin and aspirin. The indomethacin inhibition was found to be non competitive to prostaglandin E having a K(i) = 1.4 x 10M and a K'(i) = 1.6 x 10M.

AB - 15 Hydroxyprostaglandin dehydrogenase from bovine lung was purified 7.4 times to a specific activity of 1.4 mU/mg of protein. The isoelectric point was estimated at 5.4 and the molecular weight by gel filtration at 40,000. K(m) for prostaglandin E and for NAD was found to be 3.4 µM and 1.1 x 10M respectively. The enzyme was inhibited by indomethacin and aspirin. The indomethacin inhibition was found to be non competitive to prostaglandin E having a K(i) = 1.4 x 10M and a K'(i) = 1.6 x 10M.

UR - http://www.scopus.com/inward/record.url?scp=0016295885&partnerID=8YFLogxK

M3 - Journal article

AN - SCOPUS:0016295885

SN - 0142-8284

VL - 8

SP - 95

EP - 105

JO - Prostaglandins - Biology

JF - Prostaglandins - Biology

IS - 2

ER -