Influence of putative exopolysaccharide genes on Pseudomonas putida KT2440 biofilm stability

Martin Nilsson; Wen-Chi Chiang; Mustafa Fazli; Morten Gjermansen; Michael Givskov; Tim Tolker-Nielsen

doi:10.1111/j.1462-2920.2011.02447.x

Influence of putative exopolysaccharide genes on Pseudomonas putida KT2440 biofilm stability

Martin Nilsson, Wen-Chi Chiang, Mustafa Fazli, Morten Gjermansen, Michael Givskov, Tim Tolker-Nielsen

LUKKET: Institut for Immunologi og Mikrobiologi

62 Citationer (Scopus)

Abstract

We report a study of the role of putative exopolysaccharide gene clusters in the formation and stability of Pseudomonas putida KT2440 biofilm. Two novel putative exopolysaccharide gene clusters, pea and peb, were identified, and evidence is provided that they encode products that stabilize P. putida KT2440 biofilm. The gene clusters alg and bcs, which code for proteins mediating alginate and cellulose biosynthesis, were found to play minor roles in P. putida KT2440 biofilm formation and stability under the conditions tested. A P. putida KT2440 derivative devoid of any identifiable exopolysaccharide genes was found to form biofilm with a structure similar to wild-type biofilm, but with a stability lower than that of wild-type biofilm. Based on our data, we suggest that the formation of structured P. putida KT2440 biofilm can occur in the absence of exopolysaccharides; however, exopolysaccharides play a role as structural stabilizers.

Originalsprog	Engelsk
Tidsskrift	Environmental Microbiology
Vol/bind	13
Udgave nummer	5
Sider (fra-til)	1357-69
ISSN	1462-2912
DOI	https://doi.org/10.1111/j.1462-2920.2011.02447.x
Status	Udgivet - maj 2011

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10.1111/j.1462-2920.2011.02447.x

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title = "Influence of putative exopolysaccharide genes on Pseudomonas putida KT2440 biofilm stability",

abstract = "We report a study of the role of putative exopolysaccharide gene clusters in the formation and stability of Pseudomonas putida KT2440 biofilm. Two novel putative exopolysaccharide gene clusters, pea and peb, were identified, and evidence is provided that they encode products that stabilize P. putida KT2440 biofilm. The gene clusters alg and bcs, which code for proteins mediating alginate and cellulose biosynthesis, were found to play minor roles in P. putida KT2440 biofilm formation and stability under the conditions tested. A P. putida KT2440 derivative devoid of any identifiable exopolysaccharide genes was found to form biofilm with a structure similar to wild-type biofilm, but with a stability lower than that of wild-type biofilm. Based on our data, we suggest that the formation of structured P. putida KT2440 biofilm can occur in the absence of exopolysaccharides; however, exopolysaccharides play a role as structural stabilizers.",

keywords = "Alginates, Biofilms, Cellulose, Gene Knockout Techniques, Glucuronic Acid, Hexuronic Acids, Multigene Family, Mutation, Plasmids, Polysaccharides, Bacterial, Pseudomonas putida",

author = "Martin Nilsson and Wen-Chi Chiang and Mustafa Fazli and Morten Gjermansen and Michael Givskov and Tim Tolker-Nielsen",

note = "{\textcopyright} 2011 Society for Applied Microbiology and Blackwell Publishing Ltd.",

year = "2011",

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doi = "10.1111/j.1462-2920.2011.02447.x",

language = "English",

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T1 - Influence of putative exopolysaccharide genes on Pseudomonas putida KT2440 biofilm stability

AU - Nilsson, Martin

AU - Chiang, Wen-Chi

AU - Fazli, Mustafa

AU - Gjermansen, Morten

AU - Givskov, Michael

AU - Tolker-Nielsen, Tim

PY - 2011/5

Y1 - 2011/5

N2 - We report a study of the role of putative exopolysaccharide gene clusters in the formation and stability of Pseudomonas putida KT2440 biofilm. Two novel putative exopolysaccharide gene clusters, pea and peb, were identified, and evidence is provided that they encode products that stabilize P. putida KT2440 biofilm. The gene clusters alg and bcs, which code for proteins mediating alginate and cellulose biosynthesis, were found to play minor roles in P. putida KT2440 biofilm formation and stability under the conditions tested. A P. putida KT2440 derivative devoid of any identifiable exopolysaccharide genes was found to form biofilm with a structure similar to wild-type biofilm, but with a stability lower than that of wild-type biofilm. Based on our data, we suggest that the formation of structured P. putida KT2440 biofilm can occur in the absence of exopolysaccharides; however, exopolysaccharides play a role as structural stabilizers.

AB - We report a study of the role of putative exopolysaccharide gene clusters in the formation and stability of Pseudomonas putida KT2440 biofilm. Two novel putative exopolysaccharide gene clusters, pea and peb, were identified, and evidence is provided that they encode products that stabilize P. putida KT2440 biofilm. The gene clusters alg and bcs, which code for proteins mediating alginate and cellulose biosynthesis, were found to play minor roles in P. putida KT2440 biofilm formation and stability under the conditions tested. A P. putida KT2440 derivative devoid of any identifiable exopolysaccharide genes was found to form biofilm with a structure similar to wild-type biofilm, but with a stability lower than that of wild-type biofilm. Based on our data, we suggest that the formation of structured P. putida KT2440 biofilm can occur in the absence of exopolysaccharides; however, exopolysaccharides play a role as structural stabilizers.

KW - Alginates

KW - Biofilms

KW - Cellulose

KW - Gene Knockout Techniques

KW - Glucuronic Acid

KW - Hexuronic Acids

KW - Multigene Family

KW - Mutation

KW - Plasmids

KW - Polysaccharides, Bacterial

KW - Pseudomonas putida

U2 - 10.1111/j.1462-2920.2011.02447.x

DO - 10.1111/j.1462-2920.2011.02447.x

M3 - Journal article

C2 - 21507178

SN - 1462-2912

VL - 13

SP - 1357

EP - 1369

JO - Environmental Microbiology

JF - Environmental Microbiology

IS - 5

ER -

Influence of putative exopolysaccharide genes on Pseudomonas putida KT2440 biofilm stability

Abstract

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