TY - JOUR
T1 - Influence of acylation on the adsorption of insulin to hydrophobic surfaces
AU - Pinholt, Charlotte
AU - Hostrup, Susanne
AU - Bukrinsky, Jens Thostrup
AU - Frøkjær, Sven
AU - Jørgensen, Lene
PY - 2011/5
Y1 - 2011/5
N2 - Purpose: To study the effect of acylation on the adsorption of insulin to hydrophobic polystyrene beads. Methods: Adsorption isotherms for adsorption of insulin and acylated insulin to hydrophobic polystyrene beads were established, and the adsorption of the two proteins was compared further with isothermal titration calorimetry. In addition, the secondary structure and the association behavior of the two proteins were studied with circular dichroism. Results: Insulin and acylated insulin adsorbed with high affinity to the hydrophobic polystyrene beads. More acylated insulin molecules than insulin molecules adsorbed per unit surface area from solutions containing monomer-dimer mixtures of acylated insulin and insulin, respectively. In contrast, no difference was observed in the number of insulin and acylated insulin molecules adsorbing per unit surface area, when adsorption occurred from solutions containing monomer-dimer-hexamer mixtures. Conclusion: The influence of acylation on the adsorption behavior of insulin depends on the association degree of insulin, possibly due to a greater difference in hydrophobicity between monomeric insulin and acylated insulin than between the hexameric forms of these two proteins.
AB - Purpose: To study the effect of acylation on the adsorption of insulin to hydrophobic polystyrene beads. Methods: Adsorption isotherms for adsorption of insulin and acylated insulin to hydrophobic polystyrene beads were established, and the adsorption of the two proteins was compared further with isothermal titration calorimetry. In addition, the secondary structure and the association behavior of the two proteins were studied with circular dichroism. Results: Insulin and acylated insulin adsorbed with high affinity to the hydrophobic polystyrene beads. More acylated insulin molecules than insulin molecules adsorbed per unit surface area from solutions containing monomer-dimer mixtures of acylated insulin and insulin, respectively. In contrast, no difference was observed in the number of insulin and acylated insulin molecules adsorbing per unit surface area, when adsorption occurred from solutions containing monomer-dimer-hexamer mixtures. Conclusion: The influence of acylation on the adsorption behavior of insulin depends on the association degree of insulin, possibly due to a greater difference in hydrophobicity between monomeric insulin and acylated insulin than between the hexameric forms of these two proteins.
KW - Former Faculty of Pharmaceutical Sciences
U2 - 10.1007/s11095-010-0349-6
DO - 10.1007/s11095-010-0349-6
M3 - Journal article
C2 - 21190067
SN - 0724-8741
VL - 28
SP - 1031
EP - 1040
JO - Pharmaceutical Research
JF - Pharmaceutical Research
ER -