Infection with human H1N1 influenza virus affects the expression of sialic acids of metaplastic mucous cells in the ferret airways

TY - JOUR

T1 - Infection with human H1N1 influenza virus affects the expression of sialic acids of metaplastic mucous cells in the ferret airways

AU - Kirkeby, Svend

AU - Martel, Cyril Jean-Marie

AU - Aasted, Bent

PY - 2009

Y1 - 2009

N2 - Glycans terminating in sialic acids serve as receptors for influenza viruses. In this study ferrets were infected with influenza virus A/New Caledonia/20/99, and the in situ localization of sialic acids linked a2-3 and a2-6 in the airways was investigated in infected and non-infected animals by use of sialic acid detecting lectins and a monoclonal antibody towards the Sialyl-Tn antigen. The goblet cells in the bronchi from non-infected ferrets expressed Sialyla2-6Gal glycans, while the seromucinous glands in the submucosa expressed Sialyla2-3Gal glycans. In the infected animals, the surface epithelial cells in some bronchi showed metaplasia and expressed the Sialyl-Tn antigen: Sialyla2-6GalNAc-O-Thr/Ser. The submucosal tracheal glands in these animals showed increased expression of both Sialyla2-3 and Sialyla2-6 epitopes.

AB - Glycans terminating in sialic acids serve as receptors for influenza viruses. In this study ferrets were infected with influenza virus A/New Caledonia/20/99, and the in situ localization of sialic acids linked a2-3 and a2-6 in the airways was investigated in infected and non-infected animals by use of sialic acid detecting lectins and a monoclonal antibody towards the Sialyl-Tn antigen. The goblet cells in the bronchi from non-infected ferrets expressed Sialyla2-6Gal glycans, while the seromucinous glands in the submucosa expressed Sialyla2-3Gal glycans. In the infected animals, the surface epithelial cells in some bronchi showed metaplasia and expressed the Sialyl-Tn antigen: Sialyla2-6GalNAc-O-Thr/Ser. The submucosal tracheal glands in these animals showed increased expression of both Sialyla2-3 and Sialyla2-6 epitopes.

U2 - 10.1016/j.virusres.2009.05.004

DO - 10.1016/j.virusres.2009.05.004

M3 - Journal article

C2 - 19447147

SN - 0168-1702

VL - 144

SP - 225

EP - 232

JO - Virus Research

JF - Virus Research

IS - 1-2

ER -