Increased oxidation of RNA despite reduced mitochondrial respiration after chronic electroconvulsive stimulation of rat brain tissue

TY - JOUR

T1 - Increased oxidation of RNA despite reduced mitochondrial respiration after chronic electroconvulsive stimulation of rat brain tissue

AU - Nielsen, Birgitte

AU - Cejvanovic, Vanja

AU - Wortwein, Gitta

AU - Hansen, Allan R.

AU - Marstal, Kasper K.

AU - Weimann, Allan

AU - Bjerring, Peter N.

AU - Dela, Flemming

AU - Poulsen, Henrik E.

AU - Jorgensen, Martin B.

PY - 2019/1/18

Y1 - 2019/1/18

N2 - Major depressive disorder (MDD) affects 350 million people worldwide and is a serious socio-economic burden. The most efficient treatment of MDD is electroconvulsive therapy (ECT), which has been shown to influence the oxidative status believed to be part of the pathophysiology of MDD. We investigated the effects of chronic electroconvulsive stimulation (ECS) on mitochondrial respiration and mitochondrial hydrogen peroxide production, RNA oxidation, and the content of mitochondria in the piriform cortex of the rat. We found reductions of mitochondrial respiration in respiratory states 2 and 3 by 33% and 32%, respectively, and a 23% reduction in electron transfer capacity. RNA oxidation, as measured by 8-oxo-7,8-dihydroguanosine, was increased by 58%, while mitochondrial production of H2O2 was unaffected. The increased oxidative stress may thus be ascribed to extra-mitochondrial sources.

AB - Major depressive disorder (MDD) affects 350 million people worldwide and is a serious socio-economic burden. The most efficient treatment of MDD is electroconvulsive therapy (ECT), which has been shown to influence the oxidative status believed to be part of the pathophysiology of MDD. We investigated the effects of chronic electroconvulsive stimulation (ECS) on mitochondrial respiration and mitochondrial hydrogen peroxide production, RNA oxidation, and the content of mitochondria in the piriform cortex of the rat. We found reductions of mitochondrial respiration in respiratory states 2 and 3 by 33% and 32%, respectively, and a 23% reduction in electron transfer capacity. RNA oxidation, as measured by 8-oxo-7,8-dihydroguanosine, was increased by 58%, while mitochondrial production of H2O2 was unaffected. The increased oxidative stress may thus be ascribed to extra-mitochondrial sources.

KW - Mitochondria

KW - Reactive oxygen species (ROS)

KW - Hydrogen peroxide

KW - Major depression

KW - 8-oxoGuo

U2 - 10.1016/j.neulet.2018.09.061

DO - 10.1016/j.neulet.2018.09.061

M3 - Journal article

C2 - 30287304

SN - 0304-3940

VL - 690

SP - 1

EP - 5

JO - Neuroscience Letters

JF - Neuroscience Letters

ER -