Increased cellular proliferation in rat skeletal muscle and tendon in response to exercise: use of FLT and PET/CT

TY - JOUR

T1 - Increased cellular proliferation in rat skeletal muscle and tendon in response to exercise: use of FLT and PET/CT

AU - Skovgaard, Dorthe Charlotte

AU - Bayer, Monika L

AU - Mackey, Abigail L

AU - Madsen, Jacob

AU - Kjaer, Michael

AU - Kjaer, Andreas

PY - 2010/12/1

Y1 - 2010/12/1

N2 - Purpose: The purpose of this study is to investigate exercise-induced cellular proliferation in rat skeletal muscle/tendon with the use of 3′-[F-18]fluoro-3′deoxythymidine (FLT) and to quantitatively study concomitant changes in the proliferation-associated factor, Ki67. Procedures: Wistar rats (n∈=∈13) performed 3 days of treadmill running. Cellular proliferation was investigated 3 days before and 48 h after the running exercise with the use of FLT and positron emission tomography/computed tomography (PET/CT). Results were compared to a sedentary control group (n∈=∈10). Image-derived standardized uptake values were calculated for Achilles tendons and calf muscles and compared to gene expression and immunohistochemical evaluations of Ki67. Results: Treadmill running induced increased uptake of FLT uptake in calf muscles (30%; p∈<∈0.001) and in Achilles tendon (21%, p∈<∈0.001). The image-derived results were supported by a correlation in calf muscle to Ki67 (protein and mRNA level), while this coherence was not found in tendon. Conclusion: FLT-PET seems to be a promising tool for imaging of exercise-induced cellular proliferation in musculo-tendinous tissue.

AB - Purpose: The purpose of this study is to investigate exercise-induced cellular proliferation in rat skeletal muscle/tendon with the use of 3′-[F-18]fluoro-3′deoxythymidine (FLT) and to quantitatively study concomitant changes in the proliferation-associated factor, Ki67. Procedures: Wistar rats (n∈=∈13) performed 3 days of treadmill running. Cellular proliferation was investigated 3 days before and 48 h after the running exercise with the use of FLT and positron emission tomography/computed tomography (PET/CT). Results were compared to a sedentary control group (n∈=∈10). Image-derived standardized uptake values were calculated for Achilles tendons and calf muscles and compared to gene expression and immunohistochemical evaluations of Ki67. Results: Treadmill running induced increased uptake of FLT uptake in calf muscles (30%; p∈<∈0.001) and in Achilles tendon (21%, p∈<∈0.001). The image-derived results were supported by a correlation in calf muscle to Ki67 (protein and mRNA level), while this coherence was not found in tendon. Conclusion: FLT-PET seems to be a promising tool for imaging of exercise-induced cellular proliferation in musculo-tendinous tissue.

U2 - 10.1007/s11307-010-0316-y

DO - 10.1007/s11307-010-0316-y

M3 - Journal article

SN - 1536-1632

VL - 12

SP - 626

EP - 634

JO - Molecular Imaging and Biology

JF - Molecular Imaging and Biology

IS - 6

ER -