Inactivation of cellular caspases by peptide-derived tryptophan and tyrosine peroxides

Mark B Hampton; Philip E Morgan; Michael Jonathan Davies

Inactivation of cellular caspases by peptide-derived tryptophan and tyrosine peroxides

Mark B Hampton, Philip E Morgan, Michael Jonathan Davies

Inflammation, Metabolism and Oxidation

53 Citationer (Scopus)

Abstract

Peroxides generated on peptides and proteins within cells, as a result of radical attack or reaction with singlet oxygen, are longer-lived than H(2)O(2) due to their poor removal by protective enzymes. These peroxides readily oxidize cysteine residues and can inactivate thiol-dependent enzymes. We show here that Trp- and Tyr-derived peptide peroxides, generated by singlet oxygen, inhibit caspase activity in the lysates of apoptotic Jurkat cells. N-Ac-Trp-OMe peroxide was the most effective inhibitor, and was 30-fold more effective than H(2)O(2) under identical conditions. As such, protein peroxides could modulate the progression of apoptosis in cells in which they are generated.

Originalsprog	Engelsk
Tidsskrift	FEBS Letters
Vol/bind	527
Udgave nummer	1-3
Sider (fra-til)	289-92
Antal sider	4
ISSN	0014-5793
Status	Udgivet - 11 sep. 2002

Citationsformater

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title = "Inactivation of cellular caspases by peptide-derived tryptophan and tyrosine peroxides",

abstract = "Peroxides generated on peptides and proteins within cells, as a result of radical attack or reaction with singlet oxygen, are longer-lived than H(2)O(2) due to their poor removal by protective enzymes. These peroxides readily oxidize cysteine residues and can inactivate thiol-dependent enzymes. We show here that Trp- and Tyr-derived peptide peroxides, generated by singlet oxygen, inhibit caspase activity in the lysates of apoptotic Jurkat cells. N-Ac-Trp-OMe peroxide was the most effective inhibitor, and was 30-fold more effective than H(2)O(2) under identical conditions. As such, protein peroxides could modulate the progression of apoptosis in cells in which they are generated.",

keywords = "Amino Acids, Caspase Inhibitors, Cysteine Proteinase Inhibitors, Dose-Response Relationship, Drug, Enzyme Activation, Humans, Hydrogen Peroxide, Jurkat Cells, Peroxides, Tryptophan, Tyrosine",

author = "Hampton, {Mark B} and Morgan, {Philip E} and Davies, {Michael Jonathan}",

year = "2002",

month = sep,

day = "11",

language = "English",

volume = "527",

pages = "289--92",

journal = "F E B S Letters",

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TY - JOUR

T1 - Inactivation of cellular caspases by peptide-derived tryptophan and tyrosine peroxides

AU - Hampton, Mark B

AU - Morgan, Philip E

AU - Davies, Michael Jonathan

PY - 2002/9/11

Y1 - 2002/9/11

N2 - Peroxides generated on peptides and proteins within cells, as a result of radical attack or reaction with singlet oxygen, are longer-lived than H(2)O(2) due to their poor removal by protective enzymes. These peroxides readily oxidize cysteine residues and can inactivate thiol-dependent enzymes. We show here that Trp- and Tyr-derived peptide peroxides, generated by singlet oxygen, inhibit caspase activity in the lysates of apoptotic Jurkat cells. N-Ac-Trp-OMe peroxide was the most effective inhibitor, and was 30-fold more effective than H(2)O(2) under identical conditions. As such, protein peroxides could modulate the progression of apoptosis in cells in which they are generated.

AB - Peroxides generated on peptides and proteins within cells, as a result of radical attack or reaction with singlet oxygen, are longer-lived than H(2)O(2) due to their poor removal by protective enzymes. These peroxides readily oxidize cysteine residues and can inactivate thiol-dependent enzymes. We show here that Trp- and Tyr-derived peptide peroxides, generated by singlet oxygen, inhibit caspase activity in the lysates of apoptotic Jurkat cells. N-Ac-Trp-OMe peroxide was the most effective inhibitor, and was 30-fold more effective than H(2)O(2) under identical conditions. As such, protein peroxides could modulate the progression of apoptosis in cells in which they are generated.

KW - Amino Acids

KW - Caspase Inhibitors

KW - Cysteine Proteinase Inhibitors

KW - Dose-Response Relationship, Drug

KW - Enzyme Activation

KW - Humans

KW - Hydrogen Peroxide

KW - Jurkat Cells

KW - Peroxides

KW - Tryptophan

KW - Tyrosine

M3 - Journal article

C2 - 12220676

SN - 0014-5793

VL - 527

SP - 289

EP - 292

JO - F E B S Letters

JF - F E B S Letters

IS - 1-3

ER -

Inactivation of cellular caspases by peptide-derived tryptophan and tyrosine peroxides

Abstract

Fingeraftryk

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