In vitro neutralization assay using cultured hepatitis C virus

Jannick Prentoe; Jens Bukh

doi:10.1007/978-1-4939-8976-8_29

In vitro neutralization assay using cultured hepatitis C virus

Jannick Prentoe, Jens Bukh^*

^*Corresponding author af dette arbejde

4 Citationer (Scopus)

Abstract

The method outlined here enables evaluation of the neutralization potency of monoclonal and polyclonal antibodies against in vitro cultured hepatitis C virus (HCV). The high variation in envelope protein sequence among HCV isolates necessitates the inclusion of several isolates, spanning the major genotypes of HCV, in order to make strong conclusions concerning the cross-reactive neutralization potential of a given antibody. This would be particularly relevant for any neutralization experiments aimed at uncovering novel therapeutic- or vaccine-relevant antibodies. In addition, these assays can also be used to compare neutralization sensitivity of novel cultured HCV to that of previously characterized isolates.

Originalsprog	Engelsk
Titel	Hepatitis C Virus Protocols
Antal sider	7
Forlag	Humana Press
Publikationsdato	2019
Sider	433-439
ISBN (Trykt)	978-1-4939-8975-1
ISBN (Elektronisk)	978-1-4939-8976-8
DOI	https://doi.org/10.1007/978-1-4939-8976-8_29
Status	Udgivet - 2019

Navn	Methods in Molecular Biology
Vol/bind	1911
ISSN	1064-3745

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10.1007/978-1-4939-8976-8_29

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abstract = "The method outlined here enables evaluation of the neutralization potency of monoclonal and polyclonal antibodies against in vitro cultured hepatitis C virus (HCV). The high variation in envelope protein sequence among HCV isolates necessitates the inclusion of several isolates, spanning the major genotypes of HCV, in order to make strong conclusions concerning the cross-reactive neutralization potential of a given antibody. This would be particularly relevant for any neutralization experiments aimed at uncovering novel therapeutic- or vaccine-relevant antibodies. In addition, these assays can also be used to compare neutralization sensitivity of novel cultured HCV to that of previously characterized isolates.",

keywords = "Antibodies, HCV, Hepatitis C virus, Immunostaining, Neutralization, Vaccine",

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AU - Prentoe, Jannick

AU - Bukh, Jens

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N2 - The method outlined here enables evaluation of the neutralization potency of monoclonal and polyclonal antibodies against in vitro cultured hepatitis C virus (HCV). The high variation in envelope protein sequence among HCV isolates necessitates the inclusion of several isolates, spanning the major genotypes of HCV, in order to make strong conclusions concerning the cross-reactive neutralization potential of a given antibody. This would be particularly relevant for any neutralization experiments aimed at uncovering novel therapeutic- or vaccine-relevant antibodies. In addition, these assays can also be used to compare neutralization sensitivity of novel cultured HCV to that of previously characterized isolates.

AB - The method outlined here enables evaluation of the neutralization potency of monoclonal and polyclonal antibodies against in vitro cultured hepatitis C virus (HCV). The high variation in envelope protein sequence among HCV isolates necessitates the inclusion of several isolates, spanning the major genotypes of HCV, in order to make strong conclusions concerning the cross-reactive neutralization potential of a given antibody. This would be particularly relevant for any neutralization experiments aimed at uncovering novel therapeutic- or vaccine-relevant antibodies. In addition, these assays can also be used to compare neutralization sensitivity of novel cultured HCV to that of previously characterized isolates.

KW - Antibodies

KW - HCV

KW - Hepatitis C virus

KW - Immunostaining

KW - Neutralization

KW - Vaccine

U2 - 10.1007/978-1-4939-8976-8_29

DO - 10.1007/978-1-4939-8976-8_29

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AN - SCOPUS:85059235929

SN - 978-1-4939-8975-1

T3 - Methods in Molecular Biology

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BT - Hepatitis C Virus Protocols

PB - Humana Press

ER -

In vitro neutralization assay using cultured hepatitis C virus

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