In vitro generation of motor neuron precursors from mouse embryonic stem cells using mesoporous nanoparticles

TY - JOUR

T1 - In vitro generation of motor neuron precursors from mouse embryonic stem cells using mesoporous nanoparticles

AU - Garcia-Bennett, Alfonso E

AU - König, Niclas

AU - Abrahamsson, Ninnie

AU - Kozhevnikova, Mariya

AU - Zhou, Chunfang

AU - Trolle, Carl

AU - Pankratova, Stanislava

AU - Berezin, Vladimir

AU - Kozlova, Elena N

PY - 2014/11/1

Y1 - 2014/11/1

N2 - Aim: Stem cell-derived motor neurons (MNs) are utilized to develop replacement strategies for spinal cord disorders. Differentiation of embryonic stem cells into MN precursors involves factors and their repeated administration. We investigated if delivery of factors loaded into mesoporous nanoparticles could be effective for stem cell differentiation in vitro. Materials & methods: We used a mouse embryonic stem cell line expressing green fluorescent protein under the promoter for the MN-specific gene Hb9 to visualize the level of MN differentiation. The differentiation of stem cells was evaluated by expression of MN-specific transcription factors monitored by quantitative real-time PCR reactions and immunocytochemistry. Results: Mesoporous nanoparticles have strong affiliation to the embryoid bodies, penetrate inside the embryoid bodies and come in contact with differentiating cells. Conclusion: Repeated administration of soluble factors into a culture medium can be avoided due to a sustained release effect using mesoporous silica.

AB - Aim: Stem cell-derived motor neurons (MNs) are utilized to develop replacement strategies for spinal cord disorders. Differentiation of embryonic stem cells into MN precursors involves factors and their repeated administration. We investigated if delivery of factors loaded into mesoporous nanoparticles could be effective for stem cell differentiation in vitro. Materials & methods: We used a mouse embryonic stem cell line expressing green fluorescent protein under the promoter for the MN-specific gene Hb9 to visualize the level of MN differentiation. The differentiation of stem cells was evaluated by expression of MN-specific transcription factors monitored by quantitative real-time PCR reactions and immunocytochemistry. Results: Mesoporous nanoparticles have strong affiliation to the embryoid bodies, penetrate inside the embryoid bodies and come in contact with differentiating cells. Conclusion: Repeated administration of soluble factors into a culture medium can be avoided due to a sustained release effect using mesoporous silica.

U2 - 10.2217/nnm.14.23

DO - 10.2217/nnm.14.23

M3 - Journal article

C2 - 24661257

SN - 1743-5889

VL - 9

SP - 2457

EP - 2466

JO - Nanomedicine

JF - Nanomedicine

IS - 16

ER -