In vitro functional test of two subclasses of an anti-RhD antibody produced by transient expression in COS cells

Leif Kofoed Nielsen; Lars Norderhaug; Inger Sandlie; Morten Hanefeld Dziegiel

doi:10.1111/j.1600-0463.2006.apm_381.x

In vitro functional test of two subclasses of an anti-RhD antibody produced by transient expression in COS cells

Leif Kofoed Nielsen, Lars Norderhaug, Inger Sandlie, Morten Hanefeld Dziegiel

6 Citationer (Scopus)

Abstract

For over 35 years hemolytic disease of the fetus and newborn (HDFN) due to RhD has been effectively prevented by anti-RhD antibodies obtained from alloimmunized women or deliberately immunized men. However, due to the reduced number of immunized women and for ethical reasons it is foreseen that other sources of anti-RhD will be needed. One such source is recombinant human antibodies. Here we describe the construction of plasmids encoding two subclasses (IgG1 and IgG3) of an anti-RhD antibody, their transient expression in COS cells, and subsequent functional characterization of the antibodies with regard to specificity and ability to mediate a respiratory burst. The recombinant anti-RhD antibodies were specific for the RhD antigen and were able to mediate a respiratory burst. Thus these antibodies might be of use as future rhesus prophylaxis.

Originalsprog	Engelsk
Tidsskrift	APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
Vol/bind	114
Udgave nummer	5
Sider (fra-til)	345-51
Antal sider	7
ISSN	0903-4641
DOI	https://doi.org/10.1111/j.1600-0463.2006.apm_381.x
Status	Udgivet - maj 2006

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10.1111/j.1600-0463.2006.apm_381.x

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In vitro functional test of two subclasses of an anti-RhD antibody produced by transient expression in COS cells. / Nielsen, Leif Kofoed; Norderhaug, Lars; Sandlie, Inger et al.

I: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, Bind 114, Nr. 5, 05.2006, s. 345-51.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

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title = "In vitro functional test of two subclasses of an anti-RhD antibody produced by transient expression in COS cells",

abstract = "For over 35 years hemolytic disease of the fetus and newborn (HDFN) due to RhD has been effectively prevented by anti-RhD antibodies obtained from alloimmunized women or deliberately immunized men. However, due to the reduced number of immunized women and for ethical reasons it is foreseen that other sources of anti-RhD will be needed. One such source is recombinant human antibodies. Here we describe the construction of plasmids encoding two subclasses (IgG1 and IgG3) of an anti-RhD antibody, their transient expression in COS cells, and subsequent functional characterization of the antibodies with regard to specificity and ability to mediate a respiratory burst. The recombinant anti-RhD antibodies were specific for the RhD antigen and were able to mediate a respiratory burst. Thus these antibodies might be of use as future rhesus prophylaxis.",

keywords = "Animals, Antibody Specificity, COS Cells, Cercopithecus aethiops, Erythroblastosis, Fetal, Humans, Immunoglobulin G, Luminescent Measurements, Peptides, Plasmids, Protein Engineering, Recombinant Proteins, Respiratory Burst, Rh-Hr Blood-Group System, Rho(D) Immune Globulin, Transfection",

author = "Nielsen, {Leif Kofoed} and Lars Norderhaug and Inger Sandlie and Dziegiel, {Morten Hanefeld}",

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T1 - In vitro functional test of two subclasses of an anti-RhD antibody produced by transient expression in COS cells

AU - Nielsen, Leif Kofoed

AU - Norderhaug, Lars

AU - Sandlie, Inger

AU - Dziegiel, Morten Hanefeld

PY - 2006/5

Y1 - 2006/5

N2 - For over 35 years hemolytic disease of the fetus and newborn (HDFN) due to RhD has been effectively prevented by anti-RhD antibodies obtained from alloimmunized women or deliberately immunized men. However, due to the reduced number of immunized women and for ethical reasons it is foreseen that other sources of anti-RhD will be needed. One such source is recombinant human antibodies. Here we describe the construction of plasmids encoding two subclasses (IgG1 and IgG3) of an anti-RhD antibody, their transient expression in COS cells, and subsequent functional characterization of the antibodies with regard to specificity and ability to mediate a respiratory burst. The recombinant anti-RhD antibodies were specific for the RhD antigen and were able to mediate a respiratory burst. Thus these antibodies might be of use as future rhesus prophylaxis.

AB - For over 35 years hemolytic disease of the fetus and newborn (HDFN) due to RhD has been effectively prevented by anti-RhD antibodies obtained from alloimmunized women or deliberately immunized men. However, due to the reduced number of immunized women and for ethical reasons it is foreseen that other sources of anti-RhD will be needed. One such source is recombinant human antibodies. Here we describe the construction of plasmids encoding two subclasses (IgG1 and IgG3) of an anti-RhD antibody, their transient expression in COS cells, and subsequent functional characterization of the antibodies with regard to specificity and ability to mediate a respiratory burst. The recombinant anti-RhD antibodies were specific for the RhD antigen and were able to mediate a respiratory burst. Thus these antibodies might be of use as future rhesus prophylaxis.

KW - Animals

KW - Antibody Specificity

KW - COS Cells

KW - Cercopithecus aethiops

KW - Erythroblastosis, Fetal

KW - Humans

KW - Immunoglobulin G

KW - Luminescent Measurements

KW - Peptides

KW - Plasmids

KW - Protein Engineering

KW - Recombinant Proteins

KW - Respiratory Burst

KW - Rh-Hr Blood-Group System

KW - Rho(D) Immune Globulin

KW - Transfection

U2 - 10.1111/j.1600-0463.2006.apm_381.x

DO - 10.1111/j.1600-0463.2006.apm_381.x

M3 - Journal article

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SN - 0903-465X

VL - 114

SP - 345

EP - 351

JO - APMIS. Supplementum

JF - APMIS. Supplementum

IS - 5

ER -

In vitro functional test of two subclasses of an anti-RhD antibody produced by transient expression in COS cells

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