In vitro differentiation of mouse embryonic stem cells as a model of early hematopoietic development

Patrycja Sroczynska; Christophe Lancrin; Stella Pearson; Valerie Kouskoff; Georges Lacaud

doi:10.1007/978-1-59745-418-6_16

In vitro differentiation of mouse embryonic stem cells as a model of early hematopoietic development

Patrycja Sroczynska, Christophe Lancrin, Stella Pearson, Valerie Kouskoff, Georges Lacaud

40 Citationer (Scopus)

Abstract

Embryonic Stem (ES) are pluripotent cells derived from the inner cell mass of blastocysts. ES cells differentiate in vitro into all kind of cells and the development of endothelial and hematopoietic cells from mouse ES cells has been especially established. As such, the in vitro differentiation of ES cells provides a powerful experimental model to study and determine the role of specific genes in the development of the hematopoietic system. Using this approach we have demonstrated the critical function of the transcription factor AML1/Runx1 at the onset of hematopoietic development (Blood 100:458-466, 2002; Blood 103:886-889, 2004). In this chapter, we will describe our protocols and methods for the culture of healthy ES cells, their effective differentiation toward hematopoiesis, and the quantitative analysis of their hematopoietic potential by replating or gene expression analyses.

Originalsprog	Engelsk
Tidsskrift	Methods in Molecular Biology
Vol/bind	538
Sider (fra-til)	317-34
Antal sider	18
ISSN	1064-3745
DOI	https://doi.org/10.1007/978-1-59745-418-6_16
Status	Udgivet - 1 jan. 2009
Udgivet eksternt	Ja

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10.1007/978-1-59745-418-6_16

Citationsformater

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AU - Sroczynska, Patrycja

AU - Lancrin, Christophe

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AU - Kouskoff, Valerie

AU - Lacaud, Georges

PY - 2009/1/1

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N2 - Embryonic Stem (ES) are pluripotent cells derived from the inner cell mass of blastocysts. ES cells differentiate in vitro into all kind of cells and the development of endothelial and hematopoietic cells from mouse ES cells has been especially established. As such, the in vitro differentiation of ES cells provides a powerful experimental model to study and determine the role of specific genes in the development of the hematopoietic system. Using this approach we have demonstrated the critical function of the transcription factor AML1/Runx1 at the onset of hematopoietic development (Blood 100:458-466, 2002; Blood 103:886-889, 2004). In this chapter, we will describe our protocols and methods for the culture of healthy ES cells, their effective differentiation toward hematopoiesis, and the quantitative analysis of their hematopoietic potential by replating or gene expression analyses.

AB - Embryonic Stem (ES) are pluripotent cells derived from the inner cell mass of blastocysts. ES cells differentiate in vitro into all kind of cells and the development of endothelial and hematopoietic cells from mouse ES cells has been especially established. As such, the in vitro differentiation of ES cells provides a powerful experimental model to study and determine the role of specific genes in the development of the hematopoietic system. Using this approach we have demonstrated the critical function of the transcription factor AML1/Runx1 at the onset of hematopoietic development (Blood 100:458-466, 2002; Blood 103:886-889, 2004). In this chapter, we will describe our protocols and methods for the culture of healthy ES cells, their effective differentiation toward hematopoiesis, and the quantitative analysis of their hematopoietic potential by replating or gene expression analyses.

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In vitro differentiation of mouse embryonic stem cells as a model of early hematopoietic development

Abstract

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