In situ solid-state NMR spectroscopy of protein in heterogeneous membranes: the baseplate antenna complex of Chlorobaculum tepidum

Natalia Kulminskaya, Marie Ø. Pedersen, Morten Bjerring, Jarl Underhaug, Mette Miller, Niels-Ulrik Frigaard, Jakob Toudahl Nielsen, Niels Christian Nielsen

18 Citationer (Scopus)

Abstract

A clever combination: An in situ solid-state NMR analysis of CsmA proteins in the heterogeneous environment of the photoreceptor of Chlorobaculum tepidum is reported. Using different combinations of 2D and 3D solid-state NMR spectra, 90 % of the CsmA resonances are assigned and provide on the basis of chemical shift data information about the structure and conformation of CsmA in the CsmA-bacteriochlorophyll a complex (see picture).

OriginalsprogEngelsk
TidsskriftAngewandte Chemie International Edition
Vol/bind51
Udgave nummer28
Sider (fra-til)6891-6895
Antal sider5
ISSN1433-7851
DOI
StatusUdgivet - 9 jul. 2012

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