In cirrhotic patients reduced muscle strength is unrelated to muscle capacity for ATP turnover suggesting a central limitation

Christiane Marie Bourgin Gam; H B Nielsen; Niels H. Secher; F S Larsen; Peter Ott; Bjørn Quistorff

doi:10.1111/j.1475-097x.2010.00998.x

In cirrhotic patients reduced muscle strength is unrelated to muscle capacity for ATP turnover suggesting a central limitation

Christiane Marie Bourgin Gam, H B Nielsen, Niels H. Secher, F S Larsen, Peter Ott, Bjørn Quistorff

Inflammation, Metabolism and Oxidation

3 Citationer (Scopus)

Abstract

Background and aims: We investigated whether in patients with liver cirrhosis reduced muscle strength is related to dysfunction of muscle mitochondria. Methods: The mitochondrial respiratory capacity of the tibial anterior muscle was evaluated in seven patients and eight healthy control subjects by ³¹P nuclear magnetic resonance spectroscopy (³¹PMRS) to express ATP turnover in vivo and by respirometry of permeabilized fibres from the same muscle to express the in vitro capacity for oxygen consumption. Results: Maximal voluntary contraction force for plantar extension was low in the patients (46% of the control value; P<0·05), but neither the capacity for mitochondrial ATP synthesis, V_max-ATP (0·38±0·26 vs. 0·50±0·07mMs^-1; P=0·13) nor the in vitro VO_2max (0·52±0·21 vs. 0·48±0·21μmol O₂ (mingwetwt.)^-1P=0·25) were lowered correspondingly. Also, the activity of citrate synthesis and the respiratory chain complexes II and IV were similar in patients and controls. However during the contractions, the contribution to initial anaerobic ATP production from glycolysis relative to that from PCr was reduced in the patients (0·73±0·22 vs. 0·99±0·09; P<0·01). Conclusions: These results demonstrate that the markedly lower capacity for force generation in patients with liver cirrhosis is unrelated to their capacity for muscle ATP turnover, but the attenuated initial acceleration of anaerobic glycolysis suggests that these patients could be affected by a central limitation to force generation.

Originalsprog	Engelsk
Tidsskrift	Clinical Physiology and Functional Imaging
Vol/bind	31
Udgave nummer	3
Sider (fra-til)	169-74
Antal sider	6
ISSN	1475-0961
DOI	https://doi.org/10.1111/j.1475-097x.2010.00998.x
Status	Udgivet - maj 2011

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10.1111/j.1475-097x.2010.00998.x

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@article{4246725dddc44ef38eb845bc07be24d5,

title = "In cirrhotic patients reduced muscle strength is unrelated to muscle capacity for ATP turnover suggesting a central limitation",

abstract = "Background and aims: We investigated whether in patients with liver cirrhosis reduced muscle strength is related to dysfunction of muscle mitochondria. Methods: The mitochondrial respiratory capacity of the tibial anterior muscle was evaluated in seven patients and eight healthy control subjects by 31P nuclear magnetic resonance spectroscopy (31PMRS) to express ATP turnover in vivo and by respirometry of permeabilized fibres from the same muscle to express the in vitro capacity for oxygen consumption. Results: Maximal voluntary contraction force for plantar extension was low in the patients (46% of the control value; P<0·05), but neither the capacity for mitochondrial ATP synthesis, Vmax-ATP (0·38±0·26 vs. 0·50±0·07mMs-1; P=0·13) nor the in vitro VO2max (0·52±0·21 vs. 0·48±0·21μmol O2 (mingwetwt.)-1P=0·25) were lowered correspondingly. Also, the activity of citrate synthesis and the respiratory chain complexes II and IV were similar in patients and controls. However during the contractions, the contribution to initial anaerobic ATP production from glycolysis relative to that from PCr was reduced in the patients (0·73±0·22 vs. 0·99±0·09; P<0·01). Conclusions: These results demonstrate that the markedly lower capacity for force generation in patients with liver cirrhosis is unrelated to their capacity for muscle ATP turnover, but the attenuated initial acceleration of anaerobic glycolysis suggests that these patients could be affected by a central limitation to force generation.",

keywords = "Adenosine Triphosphate, Adult, Analysis of Variance, Case-Control Studies, Central Nervous System, Energy Metabolism, Female, Glycolysis, Humans, Least-Squares Analysis, Linear Models, Liver Cirrhosis, Magnetic Resonance Spectroscopy, Male, Middle Aged, Mitochondria, Muscle, Muscle Contraction, Muscle Strength, Muscle, Skeletal, Oxygen Consumption, Phosphocreatine",

author = "Gam, {Christiane Marie Bourgin} and Nielsen, {H B} and Secher, {Niels H.} and Larsen, {F S} and Peter Ott and Bj{\o}rn Quistorff",

note = "{\textcopyright} 2010 The Authors. Clinical Physiology and Functional Imaging {\textcopyright} 2010 Scandinavian Society of Clinical Physiology and Nuclear Medicine.",

year = "2011",

month = may,

doi = "10.1111/j.1475-097x.2010.00998.x",

language = "English",

volume = "31",

pages = "169--74",

journal = "Clinical Physiology and Functional Imaging",

issn = "1475-0961",

publisher = "Wiley-Blackwell",

number = "3",

}

TY - JOUR

T1 - In cirrhotic patients reduced muscle strength is unrelated to muscle capacity for ATP turnover suggesting a central limitation

AU - Gam, Christiane Marie Bourgin

AU - Nielsen, H B

AU - Secher, Niels H.

AU - Larsen, F S

AU - Ott, Peter

AU - Quistorff, Bjørn

PY - 2011/5

Y1 - 2011/5

N2 - Background and aims: We investigated whether in patients with liver cirrhosis reduced muscle strength is related to dysfunction of muscle mitochondria. Methods: The mitochondrial respiratory capacity of the tibial anterior muscle was evaluated in seven patients and eight healthy control subjects by 31P nuclear magnetic resonance spectroscopy (31PMRS) to express ATP turnover in vivo and by respirometry of permeabilized fibres from the same muscle to express the in vitro capacity for oxygen consumption. Results: Maximal voluntary contraction force for plantar extension was low in the patients (46% of the control value; P<0·05), but neither the capacity for mitochondrial ATP synthesis, Vmax-ATP (0·38±0·26 vs. 0·50±0·07mMs-1; P=0·13) nor the in vitro VO2max (0·52±0·21 vs. 0·48±0·21μmol O2 (mingwetwt.)-1P=0·25) were lowered correspondingly. Also, the activity of citrate synthesis and the respiratory chain complexes II and IV were similar in patients and controls. However during the contractions, the contribution to initial anaerobic ATP production from glycolysis relative to that from PCr was reduced in the patients (0·73±0·22 vs. 0·99±0·09; P<0·01). Conclusions: These results demonstrate that the markedly lower capacity for force generation in patients with liver cirrhosis is unrelated to their capacity for muscle ATP turnover, but the attenuated initial acceleration of anaerobic glycolysis suggests that these patients could be affected by a central limitation to force generation.

AB - Background and aims: We investigated whether in patients with liver cirrhosis reduced muscle strength is related to dysfunction of muscle mitochondria. Methods: The mitochondrial respiratory capacity of the tibial anterior muscle was evaluated in seven patients and eight healthy control subjects by 31P nuclear magnetic resonance spectroscopy (31PMRS) to express ATP turnover in vivo and by respirometry of permeabilized fibres from the same muscle to express the in vitro capacity for oxygen consumption. Results: Maximal voluntary contraction force for plantar extension was low in the patients (46% of the control value; P<0·05), but neither the capacity for mitochondrial ATP synthesis, Vmax-ATP (0·38±0·26 vs. 0·50±0·07mMs-1; P=0·13) nor the in vitro VO2max (0·52±0·21 vs. 0·48±0·21μmol O2 (mingwetwt.)-1P=0·25) were lowered correspondingly. Also, the activity of citrate synthesis and the respiratory chain complexes II and IV were similar in patients and controls. However during the contractions, the contribution to initial anaerobic ATP production from glycolysis relative to that from PCr was reduced in the patients (0·73±0·22 vs. 0·99±0·09; P<0·01). Conclusions: These results demonstrate that the markedly lower capacity for force generation in patients with liver cirrhosis is unrelated to their capacity for muscle ATP turnover, but the attenuated initial acceleration of anaerobic glycolysis suggests that these patients could be affected by a central limitation to force generation.

KW - Adenosine Triphosphate

KW - Adult

KW - Analysis of Variance

KW - Case-Control Studies

KW - Central Nervous System

KW - Energy Metabolism

KW - Female

KW - Glycolysis

KW - Humans

KW - Least-Squares Analysis

KW - Linear Models

KW - Liver Cirrhosis

KW - Magnetic Resonance Spectroscopy

KW - Male

KW - Middle Aged

KW - Mitochondria, Muscle

KW - Muscle Contraction

KW - Muscle Strength

KW - Muscle, Skeletal

KW - Oxygen Consumption

KW - Phosphocreatine

U2 - 10.1111/j.1475-097x.2010.00998.x

DO - 10.1111/j.1475-097x.2010.00998.x

M3 - Journal article

C2 - 21143366

SN - 1475-0961

VL - 31

SP - 169

EP - 174

JO - Clinical Physiology and Functional Imaging

JF - Clinical Physiology and Functional Imaging

IS - 3

ER -

In cirrhotic patients reduced muscle strength is unrelated to muscle capacity for ATP turnover suggesting a central limitation

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