Impaired embryonic haematopoiesis yet normal arterial development in the absence of the Notch ligand Jagged1

Àlex Robert-Moreno; Àlex Robert-Moreno; Jordi Guiu; Cristina Ruiz-Herguido; M. Eugenia López; Julia Inglés-Esteve; Lluis Riera; Lluis Riera; Alex Tipping; Tariq Enver; Elaine Dzierzak; Thomas Gridley; Lluis Espinosa; Anna Bigas; Anna Bigas

doi:10.1038/emboj.2008.113

Impaired embryonic haematopoiesis yet normal arterial development in the absence of the Notch ligand Jagged1

Àlex Robert-Moreno, Àlex Robert-Moreno, Jordi Guiu, Cristina Ruiz-Herguido, M. Eugenia López, Julia Inglés-Esteve, Lluis Riera, Lluis Riera, Alex Tipping, Tariq Enver, Elaine Dzierzak, Thomas Gridley, Lluis Espinosa, Anna Bigas, Anna Bigas

131 Citationer (Scopus)

Abstract

Specific deletion of Notch1 and RBPjκ in the mouse results in abrogation of definitive haematopoiesis concomitant with the loss of arterial identity at embryonic stage. As prior arterial determination is likely to be required for the generation of embryonic haematopoiesis, it is difficult to establish the specific haematopoietic role of Notch in these mutants. By analysing different Notch-ligand-null embryos, we now show that Jagged1 is not required for the establishment of the arterial fate but it is required for the correct execution of the definitive haematopoietic programme, including expression of GATA2 in the dorsal aorta. Moreover, successful haematopoietic rescue of the Jagged1-null AGM cells was obtained by culturing them with Jagged1-expressing stromal cells or by lentiviral-mediated transduction of the GATA2 gene. Taken together, our results indicate that Jagged1-mediated activation of Notch1 is responsible for regulating GATA2 expression in the AGM, which in turn is essential for definitive haematopoiesis in the mouse.

Originalsprog	Engelsk
Tidsskrift	EMBO Journal
Vol/bind	27
Udgave nummer	13
Sider (fra-til)	1886-1895
Antal sider	10
ISSN	0261-4189
DOI	https://doi.org/10.1038/emboj.2008.113
Status	Udgivet - 9 jul. 2008

Adgang til dokumentet

10.1038/emboj.2008.113

Andre filer og links

Link to publication in Scopus

Citationsformater

Robert-Moreno, À., Robert-Moreno, À., Guiu, J., Ruiz-Herguido, C., López, M. E., Inglés-Esteve, J., Riera, L., Riera, L., Tipping, A., Enver, T., Dzierzak, E., Gridley, T., Espinosa, L., Bigas, A., & Bigas, A. (2008). Impaired embryonic haematopoiesis yet normal arterial development in the absence of the Notch ligand Jagged1. EMBO Journal, 27(13), 1886-1895. https://doi.org/10.1038/emboj.2008.113

Robert-Moreno, À, Robert-Moreno, À, Guiu, J, Ruiz-Herguido, C, López, ME, Inglés-Esteve, J, Riera, L, Riera, L, Tipping, A, Enver, T, Dzierzak, E, Gridley, T, Espinosa, L, Bigas, A & Bigas, A 2008, 'Impaired embryonic haematopoiesis yet normal arterial development in the absence of the Notch ligand Jagged1', EMBO Journal, bind 27, nr. 13, s. 1886-1895. https://doi.org/10.1038/emboj.2008.113

@article{05af69526f204012b9013beeb9b5d4a9,

title = "Impaired embryonic haematopoiesis yet normal arterial development in the absence of the Notch ligand Jagged1",

abstract = "Specific deletion of Notch1 and RBPjκ in the mouse results in abrogation of definitive haematopoiesis concomitant with the loss of arterial identity at embryonic stage. As prior arterial determination is likely to be required for the generation of embryonic haematopoiesis, it is difficult to establish the specific haematopoietic role of Notch in these mutants. By analysing different Notch-ligand-null embryos, we now show that Jagged1 is not required for the establishment of the arterial fate but it is required for the correct execution of the definitive haematopoietic programme, including expression of GATA2 in the dorsal aorta. Moreover, successful haematopoietic rescue of the Jagged1-null AGM cells was obtained by culturing them with Jagged1-expressing stromal cells or by lentiviral-mediated transduction of the GATA2 gene. Taken together, our results indicate that Jagged1-mediated activation of Notch1 is responsible for regulating GATA2 expression in the AGM, which in turn is essential for definitive haematopoiesis in the mouse.",

keywords = "AGM, GATA2, Haematopoiesis, Jagged1, Notch",

author = "{\`A}lex Robert-Moreno and {\`A}lex Robert-Moreno and Jordi Guiu and Cristina Ruiz-Herguido and L{\'o}pez, {M. Eugenia} and Julia Ingl{\'e}s-Esteve and Lluis Riera and Lluis Riera and Alex Tipping and Tariq Enver and Elaine Dzierzak and Thomas Gridley and Lluis Espinosa and Anna Bigas and Anna Bigas",

year = "2008",

month = jul,

day = "9",

doi = "10.1038/emboj.2008.113",

language = "English",

volume = "27",

pages = "1886--1895",

journal = "E M B O Journal",

issn = "0261-4189",

publisher = "Wiley-Blackwell",

number = "13",

}

TY - JOUR

T1 - Impaired embryonic haematopoiesis yet normal arterial development in the absence of the Notch ligand Jagged1

AU - Robert-Moreno, Àlex

AU - Guiu, Jordi

AU - Ruiz-Herguido, Cristina

AU - López, M. Eugenia

AU - Inglés-Esteve, Julia

AU - Riera, Lluis

AU - Tipping, Alex

AU - Enver, Tariq

AU - Dzierzak, Elaine

AU - Gridley, Thomas

AU - Espinosa, Lluis

AU - Bigas, Anna

PY - 2008/7/9

Y1 - 2008/7/9

N2 - Specific deletion of Notch1 and RBPjκ in the mouse results in abrogation of definitive haematopoiesis concomitant with the loss of arterial identity at embryonic stage. As prior arterial determination is likely to be required for the generation of embryonic haematopoiesis, it is difficult to establish the specific haematopoietic role of Notch in these mutants. By analysing different Notch-ligand-null embryos, we now show that Jagged1 is not required for the establishment of the arterial fate but it is required for the correct execution of the definitive haematopoietic programme, including expression of GATA2 in the dorsal aorta. Moreover, successful haematopoietic rescue of the Jagged1-null AGM cells was obtained by culturing them with Jagged1-expressing stromal cells or by lentiviral-mediated transduction of the GATA2 gene. Taken together, our results indicate that Jagged1-mediated activation of Notch1 is responsible for regulating GATA2 expression in the AGM, which in turn is essential for definitive haematopoiesis in the mouse.

AB - Specific deletion of Notch1 and RBPjκ in the mouse results in abrogation of definitive haematopoiesis concomitant with the loss of arterial identity at embryonic stage. As prior arterial determination is likely to be required for the generation of embryonic haematopoiesis, it is difficult to establish the specific haematopoietic role of Notch in these mutants. By analysing different Notch-ligand-null embryos, we now show that Jagged1 is not required for the establishment of the arterial fate but it is required for the correct execution of the definitive haematopoietic programme, including expression of GATA2 in the dorsal aorta. Moreover, successful haematopoietic rescue of the Jagged1-null AGM cells was obtained by culturing them with Jagged1-expressing stromal cells or by lentiviral-mediated transduction of the GATA2 gene. Taken together, our results indicate that Jagged1-mediated activation of Notch1 is responsible for regulating GATA2 expression in the AGM, which in turn is essential for definitive haematopoiesis in the mouse.

KW - AGM

KW - GATA2

KW - Haematopoiesis

KW - Jagged1

KW - Notch

UR - http://www.scopus.com/inward/record.url?scp=46949110648&partnerID=8YFLogxK

U2 - 10.1038/emboj.2008.113

DO - 10.1038/emboj.2008.113

M3 - Journal article

C2 - 18528438

AN - SCOPUS:46949110648

SN - 0261-4189

VL - 27

SP - 1886

EP - 1895

JO - E M B O Journal

JF - E M B O Journal

IS - 13

ER -

Impaired embryonic haematopoiesis yet normal arterial development in the absence of the Notch ligand Jagged1

Abstract

Adgang til dokumentet

Andre filer og links

Fingeraftryk

Citationsformater