Impact of HIV and Type 2 diabetes on Gut Microbiota Diversity, Tryptophan Catabolism and Endothelial Dysfunction

Hedda Hoel; Malene Hove-Skovsgaard; Johannes R Hov; Julie Christine Gaardbo; Kristian Holm; Martin Kummen; Knut Rudi; Felix Nwosu; Jørgen Valeur; Marco Gelpi; Ingebjørg Seljeflot; Per Magne Ueland; Jan Gerstoft; Henrik Ullum; Pål Aukrust; Susanne Dam Nielsen; Marius Trøseid

doi:10.1038/s41598-018-25168-3

Impact of HIV and Type 2 diabetes on Gut Microbiota Diversity, Tryptophan Catabolism and Endothelial Dysfunction

Hedda Hoel, Malene Hove-Skovsgaard, Johannes R Hov, Julie Christine Gaardbo, Kristian Holm, Martin Kummen, Knut Rudi, Felix Nwosu, Jørgen Valeur, Marco Gelpi, Ingebjørg Seljeflot, Per Magne Ueland, Jan Gerstoft, Henrik Ullum, Pål Aukrust, Susanne Dam Nielsen, Marius Trøseid

Institut for Klinisk Medicin

17 Citationer (Scopus)

29 Downloads (Pure)

Abstract

HIV infection and type 2 diabetes are associated with altered gut microbiota, chronic inflammation, and increased cardiovascular risk. We aimed to investigate the combined effect of these diseases on gut microbiota composition and related metabolites, and a potential relation to endothelial dysfunction in individuals with HIV-infection only (n = 23), diabetes only (n = 16) or both conditions (n = 21), as well as controls (n = 24). Fecal microbiota was analyzed by Illumina sequencing of the 16 S rRNA gene. Markers of endothelial dysfunction (asymmetric dimethylarginine [ADMA]), tryptophan catabolism (kynurenine/tryptophan [KT]-ratio), and inflammation (neopterin) were measured by liquid chromatography-tandem mass spectrometry. The combination of HIV and type 2 diabetes was associated with reduced gut microbiota diversity, increased plasma KT-ratio and neopterin. Microbial genes related to tryptophan metabolism correlated with KT-ratio and low alpha diversity, in particular in HIV-infected with T2D. In multivariate analyses, KT-ratio associated with ADMA (β = 4.58 [95% CI 2.53-6.63], p < 0.001), whereas microbiota composition per se was not associated with endothelial dysfunction. Our results indicate that tryptophan catabolism may be related to endothelial dysfunction, with a potentially detrimental interaction between HIV and diabetes. The potential contribution of gut microbiota and the impact for cardiovascular risk should be further explored in prospective studies powered for clinical end points.

Originalsprog	Engelsk
Artikelnummer	6725
Tidsskrift	Scientific Reports
Vol/bind	8
Udgave nummer	1
Sider (fra-til)	1-9
Antal sider	9
ISSN	2045-2322
DOI	https://doi.org/10.1038/s41598-018-25168-3
Status	Udgivet - 1 dec. 2018

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Hoel, H., Hove-Skovsgaard, M., Hov, J. R., Gaardbo, J. C., Holm, K., Kummen, M., Rudi, K., Nwosu, F., Valeur, J., Gelpi, M., Seljeflot, I., Ueland, P. M., Gerstoft, J., Ullum, H., Aukrust, P., Nielsen, S. D., & Trøseid, M. (2018). Impact of HIV and Type 2 diabetes on Gut Microbiota Diversity, Tryptophan Catabolism and Endothelial Dysfunction. Scientific Reports, 8(1), 1-9. [6725]. https://doi.org/10.1038/s41598-018-25168-3

Hoel, H, Hove-Skovsgaard, M, Hov, JR, Gaardbo, JC, Holm, K, Kummen, M, Rudi, K, Nwosu, F, Valeur, J, Gelpi, M, Seljeflot, I, Ueland, PM, Gerstoft, J, Ullum, H, Aukrust, P, Nielsen, SD & Trøseid, M 2018, 'Impact of HIV and Type 2 diabetes on Gut Microbiota Diversity, Tryptophan Catabolism and Endothelial Dysfunction', Scientific Reports, bind 8, nr. 1, 6725, s. 1-9. https://doi.org/10.1038/s41598-018-25168-3

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abstract = "HIV infection and type 2 diabetes are associated with altered gut microbiota, chronic inflammation, and increased cardiovascular risk. We aimed to investigate the combined effect of these diseases on gut microbiota composition and related metabolites, and a potential relation to endothelial dysfunction in individuals with HIV-infection only (n = 23), diabetes only (n = 16) or both conditions (n = 21), as well as controls (n = 24). Fecal microbiota was analyzed by Illumina sequencing of the 16 S rRNA gene. Markers of endothelial dysfunction (asymmetric dimethylarginine [ADMA]), tryptophan catabolism (kynurenine/tryptophan [KT]-ratio), and inflammation (neopterin) were measured by liquid chromatography-tandem mass spectrometry. The combination of HIV and type 2 diabetes was associated with reduced gut microbiota diversity, increased plasma KT-ratio and neopterin. Microbial genes related to tryptophan metabolism correlated with KT-ratio and low alpha diversity, in particular in HIV-infected with T2D. In multivariate analyses, KT-ratio associated with ADMA (β = 4.58 [95% CI 2.53-6.63], p < 0.001), whereas microbiota composition per se was not associated with endothelial dysfunction. Our results indicate that tryptophan catabolism may be related to endothelial dysfunction, with a potentially detrimental interaction between HIV and diabetes. The potential contribution of gut microbiota and the impact for cardiovascular risk should be further explored in prospective studies powered for clinical end points.",

author = "Hedda Hoel and Malene Hove-Skovsgaard and Hov, {Johannes R} and Gaardbo, {Julie Christine} and Kristian Holm and Martin Kummen and Knut Rudi and Felix Nwosu and J{\o}rgen Valeur and Marco Gelpi and Ingebj{\o}rg Seljeflot and Ueland, {Per Magne} and Jan Gerstoft and Henrik Ullum and P{\aa}l Aukrust and Nielsen, {Susanne Dam} and Marius Tr{\o}seid",

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T1 - Impact of HIV and Type 2 diabetes on Gut Microbiota Diversity, Tryptophan Catabolism and Endothelial Dysfunction

AU - Hoel, Hedda

AU - Hove-Skovsgaard, Malene

AU - Hov, Johannes R

AU - Gaardbo, Julie Christine

AU - Holm, Kristian

AU - Kummen, Martin

AU - Rudi, Knut

AU - Nwosu, Felix

AU - Valeur, Jørgen

AU - Gelpi, Marco

AU - Seljeflot, Ingebjørg

AU - Ueland, Per Magne

AU - Gerstoft, Jan

AU - Ullum, Henrik

AU - Aukrust, Pål

AU - Nielsen, Susanne Dam

AU - Trøseid, Marius

PY - 2018/12/1

Y1 - 2018/12/1

N2 - HIV infection and type 2 diabetes are associated with altered gut microbiota, chronic inflammation, and increased cardiovascular risk. We aimed to investigate the combined effect of these diseases on gut microbiota composition and related metabolites, and a potential relation to endothelial dysfunction in individuals with HIV-infection only (n = 23), diabetes only (n = 16) or both conditions (n = 21), as well as controls (n = 24). Fecal microbiota was analyzed by Illumina sequencing of the 16 S rRNA gene. Markers of endothelial dysfunction (asymmetric dimethylarginine [ADMA]), tryptophan catabolism (kynurenine/tryptophan [KT]-ratio), and inflammation (neopterin) were measured by liquid chromatography-tandem mass spectrometry. The combination of HIV and type 2 diabetes was associated with reduced gut microbiota diversity, increased plasma KT-ratio and neopterin. Microbial genes related to tryptophan metabolism correlated with KT-ratio and low alpha diversity, in particular in HIV-infected with T2D. In multivariate analyses, KT-ratio associated with ADMA (β = 4.58 [95% CI 2.53-6.63], p < 0.001), whereas microbiota composition per se was not associated with endothelial dysfunction. Our results indicate that tryptophan catabolism may be related to endothelial dysfunction, with a potentially detrimental interaction between HIV and diabetes. The potential contribution of gut microbiota and the impact for cardiovascular risk should be further explored in prospective studies powered for clinical end points.

AB - HIV infection and type 2 diabetes are associated with altered gut microbiota, chronic inflammation, and increased cardiovascular risk. We aimed to investigate the combined effect of these diseases on gut microbiota composition and related metabolites, and a potential relation to endothelial dysfunction in individuals with HIV-infection only (n = 23), diabetes only (n = 16) or both conditions (n = 21), as well as controls (n = 24). Fecal microbiota was analyzed by Illumina sequencing of the 16 S rRNA gene. Markers of endothelial dysfunction (asymmetric dimethylarginine [ADMA]), tryptophan catabolism (kynurenine/tryptophan [KT]-ratio), and inflammation (neopterin) were measured by liquid chromatography-tandem mass spectrometry. The combination of HIV and type 2 diabetes was associated with reduced gut microbiota diversity, increased plasma KT-ratio and neopterin. Microbial genes related to tryptophan metabolism correlated with KT-ratio and low alpha diversity, in particular in HIV-infected with T2D. In multivariate analyses, KT-ratio associated with ADMA (β = 4.58 [95% CI 2.53-6.63], p < 0.001), whereas microbiota composition per se was not associated with endothelial dysfunction. Our results indicate that tryptophan catabolism may be related to endothelial dysfunction, with a potentially detrimental interaction between HIV and diabetes. The potential contribution of gut microbiota and the impact for cardiovascular risk should be further explored in prospective studies powered for clinical end points.

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DO - 10.1038/s41598-018-25168-3

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C2 - 29712976

SN - 2045-2322

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JO - Scientific Reports

JF - Scientific Reports

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ER -

Impact of HIV and Type 2 diabetes on Gut Microbiota Diversity, Tryptophan Catabolism and Endothelial Dysfunction

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