TY - JOUR
T1 - Immunosuppressive Environment in Basal Cell Carcinoma
T2 - The Role of Regulatory T Cells
AU - Omland, Silje Haukali
AU - Nielsen, Patricia S
AU - Gjerdrum, Lise M R
AU - Gniadecki, Robert
PY - 2016/11/2
Y1 - 2016/11/2
N2 - Interaction between tumour survival tactics and antitumour immune response is a major determinant for cancer growth. Regulatory T cells (T-regs) contribute to tumour immune escape, but their role in basal cell carcinoma (BCC) is not understood. The fraction of T-regs among T cells was analysed by immunohistochemistry followed by automated image analysis in facial BCC, peritumoural skin and normal, buttock skin. Quantitative real-time PCR (qRT-PCR) was performed for FOXP3 and cytokines involved in T-reg attraction and T-cell activation. T-regs comprised 45% of CD4-cells surrounding BCC. FOXP3 was highly expressed in BCC, but absent in buttock skin. Unexpectedly, expression of FOXP3 was increased in peritumoural skin, with the FOXP3/CD3 fractions exceeding those of BCC (p = 0.0065). Transforming growth factor (TGF)-β and T-reg chemokine expression was increased in BCC and peritumoural skin, but not in buttock skin, with expression levels correlating with FOXP3. T-regs are abundantly present both in BCC and in peritumoural skin, mediating an immunosuppressed microenvironment permissive for skin cancer.
AB - Interaction between tumour survival tactics and antitumour immune response is a major determinant for cancer growth. Regulatory T cells (T-regs) contribute to tumour immune escape, but their role in basal cell carcinoma (BCC) is not understood. The fraction of T-regs among T cells was analysed by immunohistochemistry followed by automated image analysis in facial BCC, peritumoural skin and normal, buttock skin. Quantitative real-time PCR (qRT-PCR) was performed for FOXP3 and cytokines involved in T-reg attraction and T-cell activation. T-regs comprised 45% of CD4-cells surrounding BCC. FOXP3 was highly expressed in BCC, but absent in buttock skin. Unexpectedly, expression of FOXP3 was increased in peritumoural skin, with the FOXP3/CD3 fractions exceeding those of BCC (p = 0.0065). Transforming growth factor (TGF)-β and T-reg chemokine expression was increased in BCC and peritumoural skin, but not in buttock skin, with expression levels correlating with FOXP3. T-regs are abundantly present both in BCC and in peritumoural skin, mediating an immunosuppressed microenvironment permissive for skin cancer.
KW - Biopsy
KW - Carcinoma, Basal Cell
KW - Cytokines
KW - Forkhead Transcription Factors
KW - Humans
KW - Immunohistochemistry
KW - Lymphocyte Activation
KW - Real-Time Polymerase Chain Reaction
KW - Skin Neoplasms
KW - T-Lymphocytes, Regulatory
KW - Journal Article
U2 - 10.2340/00015555-2440
DO - 10.2340/00015555-2440
M3 - Journal article
C2 - 27117439
SN - 0001-5555
VL - 96
SP - 917
EP - 921
JO - Acta Dermatovenereologica
JF - Acta Dermatovenereologica
IS - 7
ER -
