Immunophenotypical characterization of human neutrophil differentiation

Helena Mora Jensen; Johan Jendholm; Anna Fossum; Bo Porse; Niels Borregaad; Kim Theilgaard-Mönch

doi:10.1189/jlb.0311123

Immunophenotypical characterization of human neutrophil differentiation

Helena Mora Jensen, Johan Jendholm, Anna Fossum, Bo Porse, Niels Borregaad, Kim Theilgaard-Mönch

25 Citationer (Scopus)

Abstract

The current study reports a flow cytometry-based protocol for the prospective purification of human BM populations representing six successive stages of terminal neutrophil differentiation, including early promyelocytes and late promyelocytes, myelocytes, metamyelocytes, band cells, and PMN neutrophilic granulocytes. Validation experiments revealed a high purity of each bone marrow population and biological meaningful expression profiles for marker genes of neutrophil differentiation at a hitherto unprecedented resolution. Hence, the present protocol should be useful for studying neutrophil differentiation in vivo in the human setting and constitutes an important alternative to models that are based on in vitro differentiation of myeloid cell lines and HPCs.

Originalsprog	Engelsk
Tidsskrift	Journal of Leukocyte Biology
Sider (fra-til)	629-634
Antal sider	6
DOI	https://doi.org/10.1189/jlb.0311123
Status	Udgivet - sep. 2011

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10.1189/jlb.0311123

Citationsformater

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abstract = "The current study reports a flow cytometry-based protocol for the prospective purification of human BM populations representing six successive stages of terminal neutrophil differentiation, including early promyelocytes and late promyelocytes, myelocytes, metamyelocytes, band cells, and PMN neutrophilic granulocytes. Validation experiments revealed a high purity of each bone marrow population and biological meaningful expression profiles for marker genes of neutrophil differentiation at a hitherto unprecedented resolution. Hence, the present protocol should be useful for studying neutrophil differentiation in vivo in the human setting and constitutes an important alternative to models that are based on in vitro differentiation of myeloid cell lines and HPCs.",

author = "Jensen, {Helena Mora} and Johan Jendholm and Anna Fossum and Bo Porse and Niels Borregaad and Kim Theilgaard-M{\"o}nch",

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T1 - Immunophenotypical characterization of human neutrophil differentiation

AU - Jensen, Helena Mora

AU - Jendholm, Johan

AU - Fossum, Anna

AU - Porse, Bo

AU - Borregaad, Niels

AU - Theilgaard-Mönch, Kim

PY - 2011/9

Y1 - 2011/9

N2 - The current study reports a flow cytometry-based protocol for the prospective purification of human BM populations representing six successive stages of terminal neutrophil differentiation, including early promyelocytes and late promyelocytes, myelocytes, metamyelocytes, band cells, and PMN neutrophilic granulocytes. Validation experiments revealed a high purity of each bone marrow population and biological meaningful expression profiles for marker genes of neutrophil differentiation at a hitherto unprecedented resolution. Hence, the present protocol should be useful for studying neutrophil differentiation in vivo in the human setting and constitutes an important alternative to models that are based on in vitro differentiation of myeloid cell lines and HPCs.

AB - The current study reports a flow cytometry-based protocol for the prospective purification of human BM populations representing six successive stages of terminal neutrophil differentiation, including early promyelocytes and late promyelocytes, myelocytes, metamyelocytes, band cells, and PMN neutrophilic granulocytes. Validation experiments revealed a high purity of each bone marrow population and biological meaningful expression profiles for marker genes of neutrophil differentiation at a hitherto unprecedented resolution. Hence, the present protocol should be useful for studying neutrophil differentiation in vivo in the human setting and constitutes an important alternative to models that are based on in vitro differentiation of myeloid cell lines and HPCs.

U2 - 10.1189/jlb.0311123

DO - 10.1189/jlb.0311123

M3 - Journal article

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SN - 0741-5400

SP - 629

EP - 634

JO - Journal of Leukocyte Biology

JF - Journal of Leukocyte Biology

ER -

Immunophenotypical characterization of human neutrophil differentiation

Abstract

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