Immune responses to hair dyes containing toluene-2,5-diamine

J D Schmidt; J D Johansen; M M Nielsen; E Zimersson; C Svedman; M Bruze; K Engkilde; S S Poulsen; Carsten Geisler; C M Bonefeld

doi:10.1111/bjd.12676

Immune responses to hair dyes containing toluene-2,5-diamine

J D Schmidt, J D Johansen, M M Nielsen, E Zimersson, C Svedman, M Bruze, K Engkilde, S S Poulsen, Carsten Geisler, C M Bonefeld

11 Citationer (Scopus)

Abstract

Background Toluene-2,5-diamine (PTD) is the most frequently used dye in oxidative hair dyes on the Scandinavian market. However, little is known about immune responses to PTD-containing oxidative hair dyes. Objectives To study immune responses induced by PTD-containing hair dyes in mice. Methods Immune responses against two different permanent hair dye products containing 1·60% (w/w) and 0·48% (w/w) PTD within the colour gel, and various concentrations of pure PTD were studied. The local inflammatory response was measured by ear swelling and cell infiltration, and T- and B-cell infiltration and proliferation was determined in the draining lymph nodes. Results Concentration-dependent immune responses were seen to PTD both in the skin and draining lymph nodes. The hair dye containing 1·60% PTD induced strong local inflammation and caused T- and B-cell infiltration and proliferation as well as an increased number of regulatory T cells in the draining lymph nodes. In contrast, the hair dye containing 0·48% PTD induced skin inflammation but only minor responses in the draining lymph nodes. Conclusions Consumer-available PTD-containing permanent hair dyes can be potent immune activators inducing both pro- and anti-inflammatory responses. The outcome of the response is dependent on allergen dose, amount of additional allergens and exposure regime. What's already known about this topic? Use of permanent hair dyes can induce severe contact allergic reactions. Toluene-2,5-diamine (PTD) is more frequently used in hair dyes in the Scandinavian market than p-phenylenediamine (PPD). What does this study add? PTD-containing consumer-available hair dyes can induce severe immunological reaction in mice. The immune response to PTD-containing hair dyes is dependent on PTD concentration and additional allergens within the hair dye mixture.

Originalsprog	Engelsk
Tidsskrift	British Journal of Dermatology
Vol/bind	170
Udgave nummer	2
Sider (fra-til)	352-9
Antal sider	8
ISSN	0007-0963
DOI	https://doi.org/10.1111/bjd.12676
Status	Udgivet - feb. 2014

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10.1111/bjd.12676

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@article{cdf9cd15ee9b4c908d3b6b0850c4e18f,

title = "Immune responses to hair dyes containing toluene-2,5-diamine",

abstract = "Background Toluene-2,5-diamine (PTD) is the most frequently used dye in oxidative hair dyes on the Scandinavian market. However, little is known about immune responses to PTD-containing oxidative hair dyes. Objectives To study immune responses induced by PTD-containing hair dyes in mice. Methods Immune responses against two different permanent hair dye products containing 1·60% (w/w) and 0·48% (w/w) PTD within the colour gel, and various concentrations of pure PTD were studied. The local inflammatory response was measured by ear swelling and cell infiltration, and T- and B-cell infiltration and proliferation was determined in the draining lymph nodes. Results Concentration-dependent immune responses were seen to PTD both in the skin and draining lymph nodes. The hair dye containing 1·60% PTD induced strong local inflammation and caused T- and B-cell infiltration and proliferation as well as an increased number of regulatory T cells in the draining lymph nodes. In contrast, the hair dye containing 0·48% PTD induced skin inflammation but only minor responses in the draining lymph nodes. Conclusions Consumer-available PTD-containing permanent hair dyes can be potent immune activators inducing both pro- and anti-inflammatory responses. The outcome of the response is dependent on allergen dose, amount of additional allergens and exposure regime. What's already known about this topic? Use of permanent hair dyes can induce severe contact allergic reactions. Toluene-2,5-diamine (PTD) is more frequently used in hair dyes in the Scandinavian market than p-phenylenediamine (PPD). What does this study add? PTD-containing consumer-available hair dyes can induce severe immunological reaction in mice. The immune response to PTD-containing hair dyes is dependent on PTD concentration and additional allergens within the hair dye mixture.",

keywords = "Animals, B-Lymphocytes, Female, Hair Dyes, Immunity, Cellular, Inflammation, Mice, Inbred C57BL, Phenylenediamines, T-Lymphocytes, Regulatory",

author = "Schmidt, {J D} and Johansen, {J D} and Nielsen, {M M} and E Zimersson and C Svedman and M Bruze and K Engkilde and Poulsen, {S S} and Carsten Geisler and Bonefeld, {C M}",

note = "{\textcopyright} 2013 British Association of Dermatologists.",

year = "2014",

month = feb,

doi = "10.1111/bjd.12676",

language = "English",

volume = "170",

pages = "352--9",

journal = "British Journal of Dermatology",

issn = "0007-0963",

publisher = "Wiley-Blackwell",

number = "2",

}

TY - JOUR

T1 - Immune responses to hair dyes containing toluene-2,5-diamine

AU - Schmidt, J D

AU - Johansen, J D

AU - Nielsen, M M

AU - Zimersson, E

AU - Svedman, C

AU - Bruze, M

AU - Engkilde, K

AU - Poulsen, S S

AU - Geisler, Carsten

AU - Bonefeld, C M

PY - 2014/2

Y1 - 2014/2

N2 - Background Toluene-2,5-diamine (PTD) is the most frequently used dye in oxidative hair dyes on the Scandinavian market. However, little is known about immune responses to PTD-containing oxidative hair dyes. Objectives To study immune responses induced by PTD-containing hair dyes in mice. Methods Immune responses against two different permanent hair dye products containing 1·60% (w/w) and 0·48% (w/w) PTD within the colour gel, and various concentrations of pure PTD were studied. The local inflammatory response was measured by ear swelling and cell infiltration, and T- and B-cell infiltration and proliferation was determined in the draining lymph nodes. Results Concentration-dependent immune responses were seen to PTD both in the skin and draining lymph nodes. The hair dye containing 1·60% PTD induced strong local inflammation and caused T- and B-cell infiltration and proliferation as well as an increased number of regulatory T cells in the draining lymph nodes. In contrast, the hair dye containing 0·48% PTD induced skin inflammation but only minor responses in the draining lymph nodes. Conclusions Consumer-available PTD-containing permanent hair dyes can be potent immune activators inducing both pro- and anti-inflammatory responses. The outcome of the response is dependent on allergen dose, amount of additional allergens and exposure regime. What's already known about this topic? Use of permanent hair dyes can induce severe contact allergic reactions. Toluene-2,5-diamine (PTD) is more frequently used in hair dyes in the Scandinavian market than p-phenylenediamine (PPD). What does this study add? PTD-containing consumer-available hair dyes can induce severe immunological reaction in mice. The immune response to PTD-containing hair dyes is dependent on PTD concentration and additional allergens within the hair dye mixture.

AB - Background Toluene-2,5-diamine (PTD) is the most frequently used dye in oxidative hair dyes on the Scandinavian market. However, little is known about immune responses to PTD-containing oxidative hair dyes. Objectives To study immune responses induced by PTD-containing hair dyes in mice. Methods Immune responses against two different permanent hair dye products containing 1·60% (w/w) and 0·48% (w/w) PTD within the colour gel, and various concentrations of pure PTD were studied. The local inflammatory response was measured by ear swelling and cell infiltration, and T- and B-cell infiltration and proliferation was determined in the draining lymph nodes. Results Concentration-dependent immune responses were seen to PTD both in the skin and draining lymph nodes. The hair dye containing 1·60% PTD induced strong local inflammation and caused T- and B-cell infiltration and proliferation as well as an increased number of regulatory T cells in the draining lymph nodes. In contrast, the hair dye containing 0·48% PTD induced skin inflammation but only minor responses in the draining lymph nodes. Conclusions Consumer-available PTD-containing permanent hair dyes can be potent immune activators inducing both pro- and anti-inflammatory responses. The outcome of the response is dependent on allergen dose, amount of additional allergens and exposure regime. What's already known about this topic? Use of permanent hair dyes can induce severe contact allergic reactions. Toluene-2,5-diamine (PTD) is more frequently used in hair dyes in the Scandinavian market than p-phenylenediamine (PPD). What does this study add? PTD-containing consumer-available hair dyes can induce severe immunological reaction in mice. The immune response to PTD-containing hair dyes is dependent on PTD concentration and additional allergens within the hair dye mixture.

KW - Animals

KW - B-Lymphocytes

KW - Female

KW - Hair Dyes

KW - Immunity, Cellular

KW - Inflammation

KW - Mice, Inbred C57BL

KW - Phenylenediamines

KW - T-Lymphocytes, Regulatory

U2 - 10.1111/bjd.12676

DO - 10.1111/bjd.12676

M3 - Journal article

C2 - 24124869

SN - 0007-0963

VL - 170

SP - 352

EP - 359

JO - British Journal of Dermatology

JF - British Journal of Dermatology

IS - 2

ER -

Immune responses to hair dyes containing toluene-2,5-diamine

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