Imaging N-acyl homoserine lactone quorum sensing in vivo

TY - JOUR

T1 - Imaging N-acyl homoserine lactone quorum sensing in vivo

AU - Christensen, Louise Dahl

AU - van Gennip, Maria

AU - Jakobsen, Tim Holm

AU - Givskov, Michael

AU - Bjarnsholt, Thomas

PY - 2011/1/1

Y1 - 2011/1/1

N2 - In order to study N-acyl homoserine lactone (AHL)-based quorum sensing in vivo, we present a protocol using an Escherichia coli strain equipped with a luxR-based monitor system, which in the presence of exogenous AHL molecules expresses a green fluorescent protein (GFP). Lungs from mice challenged intratracheally with alginate beads containing both a P. aeruginosa strain together with the E. coli monitor strain can be investigated at different time points postinfection. Epifluorescent or confocal scanning laser microscopy (CSLM) is used to detect the GFP-expressing E. coli monitor strain in the lung tissues, indicating production and excretion of AHLs in vivo by the infecting P. aeruginosa.

AB - In order to study N-acyl homoserine lactone (AHL)-based quorum sensing in vivo, we present a protocol using an Escherichia coli strain equipped with a luxR-based monitor system, which in the presence of exogenous AHL molecules expresses a green fluorescent protein (GFP). Lungs from mice challenged intratracheally with alginate beads containing both a P. aeruginosa strain together with the E. coli monitor strain can be investigated at different time points postinfection. Epifluorescent or confocal scanning laser microscopy (CSLM) is used to detect the GFP-expressing E. coli monitor strain in the lung tissues, indicating production and excretion of AHLs in vivo by the infecting P. aeruginosa.

U2 - 10.1007/978-1-60761-971-0_11

DO - 10.1007/978-1-60761-971-0_11

M3 - Journal article

C2 - 21031310

SN - 1064-3745

VL - 692

SP - 147

EP - 157

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

IS - 2

ER -