Imaging Gene Expression Dynamics in Pseudomonas fluorescens In5 during Interactions with the Fungus Fusarium graminearum PH-1

TY - JOUR

T1 - Imaging Gene Expression Dynamics in Pseudomonas fluorescens In5 during Interactions with the Fungus Fusarium graminearum PH-1

AU - Hennessy, Rosanna Catherine

AU - Stougaard, Peter

AU - Olsson, Stefan

PY - 2019/6/20

Y1 - 2019/6/20

N2 - Genomics, transcriptomics and metabolomics are powerful technologies for studying microbial interactions. The main drawback of these methods is the requirement for destructive sampling. We have established an alternative but complementary technique based on a microplate system combined with promoter fusions for visualizing gene expression in space and time. Here we provide a protocol for measuring spatial and temporal gene expression of a bacterial reporter strain interacting with a fungus on a solid surface.

AB - Genomics, transcriptomics and metabolomics are powerful technologies for studying microbial interactions. The main drawback of these methods is the requirement for destructive sampling. We have established an alternative but complementary technique based on a microplate system combined with promoter fusions for visualizing gene expression in space and time. Here we provide a protocol for measuring spatial and temporal gene expression of a bacterial reporter strain interacting with a fungus on a solid surface.

U2 - 10.21769/bioprotoc.3264

DO - 10.21769/bioprotoc.3264

M3 - Journal article

SN - 2331-8325

VL - 9

SP - 1

EP - 14

JO - Bio-protocol

JF - Bio-protocol

IS - 12

M1 - e3264

ER -