TY - JOUR
T1 - IL-2 and IL-15 regulate CD154 expression on activated CD4 T cells
AU - Skov, S
AU - Bonyhadi, M
AU - Odum, Niels
AU - Ledbetter, J A
N1 - Keywords: Antigen-Presenting Cells; Antigens, CD28; Antigens, CD3; Antigens, CD40; CD4-Positive T-Lymphocytes; CD40 Ligand; Calcineurin; Cell Communication; Cells, Cultured; Coculture Techniques; Humans; Interleukin-15; Interleukin-2; Ligands; Lymphocyte Activation; Membrane Glycoproteins; Time Factors; Tumor Cells, Cultured
PY - 2000
Y1 - 2000
N2 - The cellular and humoral immune system is critically dependent upon CD40-CD154 (CD40 ligand) interactions between CD40 expressed on B cells, macrophages, and dendritic cells, and CD154 expressed primarily on CD4 T cells. Previous studies have shown that CD154 is transiently expressed on CD4 T cells after T cell receptor engagement in vitro. However, we found that stimulation of PBLs with maximal CD28 costimulation, using beads coupled to Abs against CD3 and CD28, led to a very prolonged expression of CD154 on CD4 cells (>4 days) that was dependent upon autocrine IL-2 production. Previously activated CD4 T cells could respond to IL-2, or the related cytokine IL-15, by de novo CD154 production and expression without requiring an additional signal from CD3 and CD28. These results provide evidence that CD28 costimulation of CD4 T cells, through autocrine IL-2 production, maintains high levels of CD154 expression. This has significant impact on our understanding of the acquired immune response and may provide insight concerning the mechanisms underlying several immunological diseases.
AB - The cellular and humoral immune system is critically dependent upon CD40-CD154 (CD40 ligand) interactions between CD40 expressed on B cells, macrophages, and dendritic cells, and CD154 expressed primarily on CD4 T cells. Previous studies have shown that CD154 is transiently expressed on CD4 T cells after T cell receptor engagement in vitro. However, we found that stimulation of PBLs with maximal CD28 costimulation, using beads coupled to Abs against CD3 and CD28, led to a very prolonged expression of CD154 on CD4 cells (>4 days) that was dependent upon autocrine IL-2 production. Previously activated CD4 T cells could respond to IL-2, or the related cytokine IL-15, by de novo CD154 production and expression without requiring an additional signal from CD3 and CD28. These results provide evidence that CD28 costimulation of CD4 T cells, through autocrine IL-2 production, maintains high levels of CD154 expression. This has significant impact on our understanding of the acquired immune response and may provide insight concerning the mechanisms underlying several immunological diseases.
M3 - Journal article
C2 - 10725703
SN - 0022-1767
VL - 164
SP - 3500
EP - 3505
JO - Journal of Immunology
JF - Journal of Immunology
IS - 7
ER -