Identification of the entire chromosome complement of bread wheat by two-colour FISH

C. Pedersen; P. Langridge

doi:10.1139/g97-077

Identification of the entire chromosome complement of bread wheat by two-colour FISH

C. Pedersen^*, P. Langridge

^*Corresponding author af dette arbejde

Plante- og Jordvidenskab

149 Citationer (Scopus)

Abstract

Using the Aegilops tauschii clone pAs1 together with the barley clone pHvG38 for two-colour fluorescence in situ hybridization (FISH) the entire chromosome complement of hexaploid wheat was identified. The combination of the two probes allowed easy discrimination of the three genomes of wheat. The banding pattern obtained with the pHvG38 probe containing the GAA-satellite sequence was identical to the N-banding pattern of wheat. A detailed idiogram was constructed, including 73 GAA bands and 48 pAs1 bands. Identification of the wheat chromosomes by FISH will be particularly useful in connection with the physical mapping of other DNA sequences to chromosomes, or for chromosome identification in general, as an alternative to C-banding.

Originalsprog	Engelsk
Tidsskrift	Genome
Vol/bind	40
Udgave nummer	5
Sider (fra-til)	589-593
Antal sider	5
ISSN	0831-2796
DOI	https://doi.org/10.1139/g97-077
Status	Udgivet - 1 jan. 1997

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title = "Identification of the entire chromosome complement of bread wheat by two-colour FISH",

abstract = "Using the Aegilops tauschii clone pAs1 together with the barley clone pHvG38 for two-colour fluorescence in situ hybridization (FISH) the entire chromosome complement of hexaploid wheat was identified. The combination of the two probes allowed easy discrimination of the three genomes of wheat. The banding pattern obtained with the pHvG38 probe containing the GAA-satellite sequence was identical to the N-banding pattern of wheat. A detailed idiogram was constructed, including 73 GAA bands and 48 pAs1 bands. Identification of the wheat chromosomes by FISH will be particularly useful in connection with the physical mapping of other DNA sequences to chromosomes, or for chromosome identification in general, as an alternative to C-banding.",

keywords = "Chromosome identification, Fluorescence in situ hybridization, Repetitive DNA sequences, Triticum aestivum",

author = "C. Pedersen and P. Langridge",

year = "1997",

month = jan,

day = "1",

doi = "10.1139/g97-077",

language = "English",

volume = "40",

pages = "589--593",

journal = "Genome",

issn = "0831-2796",

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TY - JOUR

T1 - Identification of the entire chromosome complement of bread wheat by two-colour FISH

AU - Pedersen, C.

AU - Langridge, P.

PY - 1997/1/1

Y1 - 1997/1/1

N2 - Using the Aegilops tauschii clone pAs1 together with the barley clone pHvG38 for two-colour fluorescence in situ hybridization (FISH) the entire chromosome complement of hexaploid wheat was identified. The combination of the two probes allowed easy discrimination of the three genomes of wheat. The banding pattern obtained with the pHvG38 probe containing the GAA-satellite sequence was identical to the N-banding pattern of wheat. A detailed idiogram was constructed, including 73 GAA bands and 48 pAs1 bands. Identification of the wheat chromosomes by FISH will be particularly useful in connection with the physical mapping of other DNA sequences to chromosomes, or for chromosome identification in general, as an alternative to C-banding.

AB - Using the Aegilops tauschii clone pAs1 together with the barley clone pHvG38 for two-colour fluorescence in situ hybridization (FISH) the entire chromosome complement of hexaploid wheat was identified. The combination of the two probes allowed easy discrimination of the three genomes of wheat. The banding pattern obtained with the pHvG38 probe containing the GAA-satellite sequence was identical to the N-banding pattern of wheat. A detailed idiogram was constructed, including 73 GAA bands and 48 pAs1 bands. Identification of the wheat chromosomes by FISH will be particularly useful in connection with the physical mapping of other DNA sequences to chromosomes, or for chromosome identification in general, as an alternative to C-banding.

KW - Chromosome identification

KW - Fluorescence in situ hybridization

KW - Repetitive DNA sequences

KW - Triticum aestivum

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U2 - 10.1139/g97-077

DO - 10.1139/g97-077

M3 - Journal article

AN - SCOPUS:0031430867

SN - 0831-2796

VL - 40

SP - 589

EP - 593

JO - Genome

JF - Genome

IS - 5

ER -

Identification of the entire chromosome complement of bread wheat by two-colour FISH

Abstract

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