@article{e4588e30d94211dcbee902004c4f4f50,
title = "Identification of miRNA targets with stable isotope labeling by amino acids in cell culture.",
abstract = "miRNAs are small noncoding RNAs that regulate gene expression. We have used stable isotope labeling by amino acids in cell culture (SILAC) to investigate the effect of miRNA-1 on the HeLa cell proteome. Expression of 12 out of 504 investigated proteins was repressed by miRNA-1 transfection. This repressed set of genes significantly overlaps with miRNA-1 regulated genes that have been identified with DNA array technology and are predicted by computational methods. Moreover, we find that the 3'-untranslated region for the repressed set are enriched in miRNA-1 complementary sites. Our findings demonstrate that SILAC can be used for miRNA target identification and that one highly expressed miRNA can regulate the levels of many different proteins. ",
author = "Jeppe Vinther and Hedegaard, {Mads Marquardt} and Gardner, {Paul Phillip} and Andersen, {Jens S.} and Peter Arctander",
year = "2006",
doi = "10.1093/nar/gkl590",
language = "English",
volume = "34",
pages = "e107",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "16",
}