Identification of let-7-regulated oncofetal genes.

Benjamin Boyerinas; Sun-Mi Park; Noam Shomron; Mads M Hedegaard; Jeppe Vinther; Jens S Andersen; Christine Feig; Jinbo Xu; Christopher B Burge; Marcus E Peter

doi:10.1158/0008-5472.CAN-08-0264

Identification of let-7-regulated oncofetal genes.

Benjamin Boyerinas, Sun-Mi Park, Noam Shomron, Mads M Hedegaard, Jeppe Vinther, Jens S Andersen, Christine Feig, Jinbo Xu, Christopher B Burge, Marcus E Peter

Functional genomics

172 Citationer (Scopus)

Abstract

MicroRNAs (miRNA) are small RNA molecules of approximately 20 to 22 nucleotides that reduce expression of proteins through mRNA degradation and/or translational silencing. Each known miRNA has a large number of predicted targets. Members of the let-7/miR-98 family of miRNAs are up-regulated at the end of embryonic development. Let-7 is often down-regulated early during cancer development, suggesting that let-7-regulated oncofetal genes (LOG) may become reexpressed in cancer cells. Using comparative bioinformatics, we have identified 12 conserved LOGs that include HMGA2 and IMP-1/CRD-BP. IMP-1 has growth-promoting activities through stabilization of c-myc mRNA. We experimentally confirmed that IMP-1 is a direct let-7 target that promotes cell growth and motility of tumor cells, and we confirmed by proteomics analysis that IMP-1 and HMGA2 are major miRNA targets. Our data suggest that a substantial part of the growth inhibitory activities of let-7 comes from suppressing the expression of IMP-1. LOGs could be novel therapeutic targets and potential biomarkers for cancer treatment.
Udgivelsesdato: 2008-Apr-15

Originalsprog	Engelsk
Tidsskrift	Cancer Research
Vol/bind	68
Udgave nummer	8
Sider (fra-til)	2587-91
Antal sider	4
ISSN	0008-5472
DOI	https://doi.org/10.1158/0008-5472.CAN-08-0264
Status	Udgivet - 2008

Adgang til dokumentet

10.1158/0008-5472.CAN-08-0264

Citationsformater

@article{d6d0404014f811ddbee902004c4f4f50,

title = "Identification of let-7-regulated oncofetal genes.",

abstract = "MicroRNAs (miRNA) are small RNA molecules of approximately 20 to 22 nucleotides that reduce expression of proteins through mRNA degradation and/or translational silencing. Each known miRNA has a large number of predicted targets. Members of the let-7/miR-98 family of miRNAs are up-regulated at the end of embryonic development. Let-7 is often down-regulated early during cancer development, suggesting that let-7-regulated oncofetal genes (LOG) may become reexpressed in cancer cells. Using comparative bioinformatics, we have identified 12 conserved LOGs that include HMGA2 and IMP-1/CRD-BP. IMP-1 has growth-promoting activities through stabilization of c-myc mRNA. We experimentally confirmed that IMP-1 is a direct let-7 target that promotes cell growth and motility of tumor cells, and we confirmed by proteomics analysis that IMP-1 and HMGA2 are major miRNA targets. Our data suggest that a substantial part of the growth inhibitory activities of let-7 comes from suppressing the expression of IMP-1. LOGs could be novel therapeutic targets and potential biomarkers for cancer treatment. Udgivelsesdato: 2008-Apr-15",

author = "Benjamin Boyerinas and Sun-Mi Park and Noam Shomron and Hedegaard, {Mads M} and Jeppe Vinther and Andersen, {Jens S} and Christine Feig and Jinbo Xu and Burge, {Christopher B} and Peter, {Marcus E}",

note = "Key Words: cancer progression • IMP-1 • miRNA",

year = "2008",

doi = "10.1158/0008-5472.CAN-08-0264",

language = "English",

volume = "68",

pages = "2587--91",

journal = "Cancer Research",

issn = "0008-5472",

publisher = "American Association for Cancer Research",

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TY - JOUR

T1 - Identification of let-7-regulated oncofetal genes.

AU - Boyerinas, Benjamin

AU - Park, Sun-Mi

AU - Shomron, Noam

AU - Hedegaard, Mads M

AU - Vinther, Jeppe

AU - Andersen, Jens S

AU - Feig, Christine

AU - Xu, Jinbo

AU - Burge, Christopher B

AU - Peter, Marcus E

N1 - Key Words: cancer progression • IMP-1 • miRNA

PY - 2008

Y1 - 2008

N2 - MicroRNAs (miRNA) are small RNA molecules of approximately 20 to 22 nucleotides that reduce expression of proteins through mRNA degradation and/or translational silencing. Each known miRNA has a large number of predicted targets. Members of the let-7/miR-98 family of miRNAs are up-regulated at the end of embryonic development. Let-7 is often down-regulated early during cancer development, suggesting that let-7-regulated oncofetal genes (LOG) may become reexpressed in cancer cells. Using comparative bioinformatics, we have identified 12 conserved LOGs that include HMGA2 and IMP-1/CRD-BP. IMP-1 has growth-promoting activities through stabilization of c-myc mRNA. We experimentally confirmed that IMP-1 is a direct let-7 target that promotes cell growth and motility of tumor cells, and we confirmed by proteomics analysis that IMP-1 and HMGA2 are major miRNA targets. Our data suggest that a substantial part of the growth inhibitory activities of let-7 comes from suppressing the expression of IMP-1. LOGs could be novel therapeutic targets and potential biomarkers for cancer treatment. Udgivelsesdato: 2008-Apr-15

AB - MicroRNAs (miRNA) are small RNA molecules of approximately 20 to 22 nucleotides that reduce expression of proteins through mRNA degradation and/or translational silencing. Each known miRNA has a large number of predicted targets. Members of the let-7/miR-98 family of miRNAs are up-regulated at the end of embryonic development. Let-7 is often down-regulated early during cancer development, suggesting that let-7-regulated oncofetal genes (LOG) may become reexpressed in cancer cells. Using comparative bioinformatics, we have identified 12 conserved LOGs that include HMGA2 and IMP-1/CRD-BP. IMP-1 has growth-promoting activities through stabilization of c-myc mRNA. We experimentally confirmed that IMP-1 is a direct let-7 target that promotes cell growth and motility of tumor cells, and we confirmed by proteomics analysis that IMP-1 and HMGA2 are major miRNA targets. Our data suggest that a substantial part of the growth inhibitory activities of let-7 comes from suppressing the expression of IMP-1. LOGs could be novel therapeutic targets and potential biomarkers for cancer treatment. Udgivelsesdato: 2008-Apr-15

U2 - 10.1158/0008-5472.CAN-08-0264

DO - 10.1158/0008-5472.CAN-08-0264

M3 - Journal article

C2 - 18413726

SN - 0008-5472

VL - 68

SP - 2587

EP - 2591

JO - Cancer Research

JF - Cancer Research

IS - 8

ER -