Identification of four evolutionarily related G protein-coupled receptors from the malaria mosquito Anopheles gambiae

TY - JOUR

T1 - Identification of four evolutionarily related G protein-coupled receptors from the malaria mosquito Anopheles gambiae

AU - Belmont, Martin

AU - Cazzamali, Giuseppe

AU - Williamson, Michael

AU - Hauser, Frank

AU - Grimmelikhuijzen, Cornelis J P

PY - 2006/5/26

Y1 - 2006/5/26

N2 - The mosquito Anopheles gambiae is an important vector for malaria, which is one of the most serious human parasitic diseases in the world, causing up to 2.7 million deaths yearly. To contribute to our understanding of A. gambiae and to the transmission of malaria, we have now cloned four evolutionarily related G protein-coupled receptors (GPCRs) from this mosquito and expressed them in Chinese hamster ovary cells. After screening of a library of thirty-three insect or other invertebrate neuropeptides and eight biogenic amines, we could identify (de-orphanize) three of these GPCRs as: an adipokinetic hormone (AKH) receptor (EC(50) for A. gambiae AKH, 3x10(-9)M), a corazonin receptor (EC(50) for A. gambiae corazonin, 4x10(-9)M), and a crustacean cardioactive peptide (CCAP) receptor (EC(50) for A. gambiae CCAP, 1x10(-9)M). The fourth GPCR remained an orphan, although its close evolutionary relationship to the A. gambiae and other insect AKH receptors suggested that it is a receptor for an AKH-like peptide. This is the first published report on evolutionarily related AKH, corazonin, and CCAP receptors in mosquitoes.

AB - The mosquito Anopheles gambiae is an important vector for malaria, which is one of the most serious human parasitic diseases in the world, causing up to 2.7 million deaths yearly. To contribute to our understanding of A. gambiae and to the transmission of malaria, we have now cloned four evolutionarily related G protein-coupled receptors (GPCRs) from this mosquito and expressed them in Chinese hamster ovary cells. After screening of a library of thirty-three insect or other invertebrate neuropeptides and eight biogenic amines, we could identify (de-orphanize) three of these GPCRs as: an adipokinetic hormone (AKH) receptor (EC(50) for A. gambiae AKH, 3x10(-9)M), a corazonin receptor (EC(50) for A. gambiae corazonin, 4x10(-9)M), and a crustacean cardioactive peptide (CCAP) receptor (EC(50) for A. gambiae CCAP, 1x10(-9)M). The fourth GPCR remained an orphan, although its close evolutionary relationship to the A. gambiae and other insect AKH receptors suggested that it is a receptor for an AKH-like peptide. This is the first published report on evolutionarily related AKH, corazonin, and CCAP receptors in mosquitoes.

KW - Amino Acid Sequence

KW - Animals

KW - Anopheles gambiae

KW - CHO Cells

KW - Cloning, Molecular

KW - Cricetinae

KW - Cricetulus

KW - Evolution, Molecular

KW - Gene Order

KW - Insect Hormones

KW - Insect Proteins

KW - Insect Vectors

KW - Malaria

KW - Molecular Sequence Data

KW - Neuropeptides

KW - Oligopeptides

KW - Phylogeny

KW - Pyrrolidonecarboxylic Acid

KW - Receptors, G-Protein-Coupled

KW - Receptors, Neuropeptide

U2 - 10.1016/j.bbrc.2006.03.117

DO - 10.1016/j.bbrc.2006.03.117

M3 - Journal article

C2 - 16616003

SN - 0006-291X

VL - 344

SP - 160

EP - 165

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -