Identification of corticotropin-releasing factor (CRF) target cells and effects of dexamethasone on binding in anterior pituitary using a fluorescent analog of CRF

J Schwartz; Nils Billestrup; M Perrin; J Rivier; W Vale

doi:10.1210/endo-119-5-2376

Identification of corticotropin-releasing factor (CRF) target cells and effects of dexamethasone on binding in anterior pituitary using a fluorescent analog of CRF

J Schwartz, Nils Billestrup, M Perrin, J Rivier, W Vale

43 Citationer (Scopus)

Abstract

A fluorescein-conjugated bioactive analog of corticotropin-releasing factor (CRF) was synthesized and used to label cells that have high affinity CRF-binding sites. Of cultured bovine anterior pituitary cells, 6.1 +/- 0.6% were visible by fluorescence microscopy after incubation with the analog. Fluorescence was eliminated by coincubation with a 200-fold excess of unlabeled CRF. Treatment with dexamethasone (10(-9)-10(-7) M) decreased visible fluorescence in a dose-dependent manner. These results demonstrate the utility of a fluorescent CRF analog for identification of cells with specific CRF-binding sites and suggest that binding of CRF to anterior pituitary cells is altered by glucocorticoids.

Originalsprog	Engelsk
Tidsskrift	Endocrinology
Vol/bind	119
Udgave nummer	5
Sider (fra-til)	2376-82
Antal sider	7
ISSN	0013-7227
DOI	https://doi.org/10.1210/endo-119-5-2376
Status	Udgivet - nov. 1986

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10.1210/endo-119-5-2376

Citationsformater

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title = "Identification of corticotropin-releasing factor (CRF) target cells and effects of dexamethasone on binding in anterior pituitary using a fluorescent analog of CRF",

abstract = "A fluorescein-conjugated bioactive analog of corticotropin-releasing factor (CRF) was synthesized and used to label cells that have high affinity CRF-binding sites. Of cultured bovine anterior pituitary cells, 6.1 +/- 0.6% were visible by fluorescence microscopy after incubation with the analog. Fluorescence was eliminated by coincubation with a 200-fold excess of unlabeled CRF. Treatment with dexamethasone (10(-9)-10(-7) M) decreased visible fluorescence in a dose-dependent manner. These results demonstrate the utility of a fluorescent CRF analog for identification of cells with specific CRF-binding sites and suggest that binding of CRF to anterior pituitary cells is altered by glucocorticoids.",

keywords = "Adrenocorticotropic Hormone, Animals, Cattle, Corticotropin-Releasing Hormone, Dexamethasone, Dose-Response Relationship, Drug, Flow Cytometry, Fluorescent Dyes, Microscopy, Fluorescence, Pituitary Gland, Anterior, Rats",

author = "J Schwartz and Nils Billestrup and M Perrin and J Rivier and W Vale",

year = "1986",

month = nov,

doi = "10.1210/endo-119-5-2376",

language = "English",

volume = "119",

pages = "2376--82",

journal = "Endocrinology",

issn = "0013-7227",

publisher = "Oxford University Press",

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TY - JOUR

T1 - Identification of corticotropin-releasing factor (CRF) target cells and effects of dexamethasone on binding in anterior pituitary using a fluorescent analog of CRF

AU - Schwartz, J

AU - Billestrup, Nils

AU - Perrin, M

AU - Rivier, J

AU - Vale, W

PY - 1986/11

Y1 - 1986/11

N2 - A fluorescein-conjugated bioactive analog of corticotropin-releasing factor (CRF) was synthesized and used to label cells that have high affinity CRF-binding sites. Of cultured bovine anterior pituitary cells, 6.1 +/- 0.6% were visible by fluorescence microscopy after incubation with the analog. Fluorescence was eliminated by coincubation with a 200-fold excess of unlabeled CRF. Treatment with dexamethasone (10(-9)-10(-7) M) decreased visible fluorescence in a dose-dependent manner. These results demonstrate the utility of a fluorescent CRF analog for identification of cells with specific CRF-binding sites and suggest that binding of CRF to anterior pituitary cells is altered by glucocorticoids.

AB - A fluorescein-conjugated bioactive analog of corticotropin-releasing factor (CRF) was synthesized and used to label cells that have high affinity CRF-binding sites. Of cultured bovine anterior pituitary cells, 6.1 +/- 0.6% were visible by fluorescence microscopy after incubation with the analog. Fluorescence was eliminated by coincubation with a 200-fold excess of unlabeled CRF. Treatment with dexamethasone (10(-9)-10(-7) M) decreased visible fluorescence in a dose-dependent manner. These results demonstrate the utility of a fluorescent CRF analog for identification of cells with specific CRF-binding sites and suggest that binding of CRF to anterior pituitary cells is altered by glucocorticoids.

KW - Adrenocorticotropic Hormone

KW - Animals

KW - Cattle

KW - Corticotropin-Releasing Hormone

KW - Dexamethasone

KW - Dose-Response Relationship, Drug

KW - Flow Cytometry

KW - Fluorescent Dyes

KW - Microscopy, Fluorescence

KW - Pituitary Gland, Anterior

KW - Rats

U2 - 10.1210/endo-119-5-2376

DO - 10.1210/endo-119-5-2376

M3 - Journal article

C2 - 3021441

SN - 0013-7227

VL - 119

SP - 2376

EP - 2382

JO - Endocrinology

JF - Endocrinology

IS - 5

ER -

Identification of corticotropin-releasing factor (CRF) target cells and effects of dexamethasone on binding in anterior pituitary using a fluorescent analog of CRF

Abstract

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