Identification of continuous epitopes of HuD antibodies related to paraneoplastic diseases/small cell lung cancer

TY - JOUR

T1 - Identification of continuous epitopes of HuD antibodies related to paraneoplastic diseases/small cell lung cancer

AU - Trier, Nicole Hartwig

AU - Hansen, Paul Robert

AU - Vedeler, Christian A.

AU - Somnier, Finn E.

AU - Houen, Gunnar

PY - 2012/2/29

Y1 - 2012/2/29

N2 - HuD antibodies are associated with small cell lung cancer. To identify relevant epitopes of HuD antibodies, patient sera and a monoclonal antibody were analyzed for their reactivity to linear 20. mer peptides spanning the human HuD protein. The HuD monoclonal antibody recognized a single fragment located in the first RNA recognition motif. Thorough analysis identified VRDKITQGSL as the actual epitope. Screening of anti-HuD positive patients and healthy controls identified eight peptides as potential subdominant epitopes. The majority of these peptides were located in the N-terminal end as well as in the linker region between the second and third RNA recognition motifs.

AB - HuD antibodies are associated with small cell lung cancer. To identify relevant epitopes of HuD antibodies, patient sera and a monoclonal antibody were analyzed for their reactivity to linear 20. mer peptides spanning the human HuD protein. The HuD monoclonal antibody recognized a single fragment located in the first RNA recognition motif. Thorough analysis identified VRDKITQGSL as the actual epitope. Screening of anti-HuD positive patients and healthy controls identified eight peptides as potential subdominant epitopes. The majority of these peptides were located in the N-terminal end as well as in the linker region between the second and third RNA recognition motifs.

M3 - Journal article

SN - 0165-5728

VL - 243

SP - 25

EP - 33

JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

IS - 1

ER -