TY - JOUR
T1 - Identification of chromatophore membrane protein complexes formed under different nitrogen availability conditions in Rhodospirillum rubrum
AU - Selao, Tiago Toscano
AU - Branca, Rui
AU - Chae, Pil Seok
AU - Lehtiö, Janne
AU - Gellman, Samuel H
AU - Rasmussen, Søren Gøgsig Faarup
AU - Nordlund, Stefan
AU - Norén, Agneta
PY - 2011/6/3
Y1 - 2011/6/3
N2 - The chromatophore membrane of the photosynthetic diazotroph Rhodospirillum rubrum is of vital importance for a number of central processes, including nitrogen fixation. Using a novel amphiphile, we have identified protein complexes present under different nitrogen availability conditions by the use of two-dimensional Blue Native/SDS-PAGE and NSI-LC-LTQ-Orbitrap mass spectrometry. We have identified several membrane protein complexes, including components of the ATP synthase, reaction center, light harvesting, and NADH dehydrogenase complexes. Additionally, we have identified differentially expressed proteins, such as subunits of the succinate dehydrogenase complex and other TCA cycle enzymes that are usually found in the cytosol, thus hinting at a possible association to the membrane in response to nitrogen deficiency. We propose a redox sensing mechanism that can influence the membrane subproteome in response to nitrogen availability.
AB - The chromatophore membrane of the photosynthetic diazotroph Rhodospirillum rubrum is of vital importance for a number of central processes, including nitrogen fixation. Using a novel amphiphile, we have identified protein complexes present under different nitrogen availability conditions by the use of two-dimensional Blue Native/SDS-PAGE and NSI-LC-LTQ-Orbitrap mass spectrometry. We have identified several membrane protein complexes, including components of the ATP synthase, reaction center, light harvesting, and NADH dehydrogenase complexes. Additionally, we have identified differentially expressed proteins, such as subunits of the succinate dehydrogenase complex and other TCA cycle enzymes that are usually found in the cytosol, thus hinting at a possible association to the membrane in response to nitrogen deficiency. We propose a redox sensing mechanism that can influence the membrane subproteome in response to nitrogen availability.
KW - ATP Synthetase Complexes
KW - Ammonium Chloride
KW - Bacterial Chromatophores
KW - Citric Acid Cycle
KW - Electron Transport Complex I
KW - Electrophoresis, Gel, Two-Dimensional
KW - Flavoproteins
KW - Light-Harvesting Protein Complexes
KW - Membrane Proteins
KW - Membrane Transport Proteins
KW - Nitrogen
KW - Nitrogen Fixation
KW - Rhodospirillum rubrum
KW - Subcellular Fractions
U2 - 10.1021/pr100838x
DO - 10.1021/pr100838x
M3 - Journal article
C2 - 21443180
SN - 1535-3893
VL - 10
SP - 2703
EP - 2714
JO - Journal of Proteome Research
JF - Journal of Proteome Research
IS - 6
ER -