TY - JOUR
T1 - Beauveria bassiana as an endophyte
T2 - a critical review on associated methodology and biocontrol potential
AU - McKinnon, Aimee C.
AU - Saari, Susanna Talvikki
AU - Moran-Diez, Maria E.
AU - Meyling, Nicolai Vitt
AU - Raad, Maya
AU - Glare, Travis R.
PY - 2017
Y1 - 2017
N2 - In the last decade there has been increased focus on the potential of endophytic Beauveria bassiana for the biocontrol of insect herbivores. Generally, detection of endophytes is acknowledged to be problematic and recovery method-dependent. Herein, we critically analyse the methodology reported for the detection of B. bassiana as endophytes following experimental inoculation. In light of the methodology, we further review the effects of endophytic B. bassiana on insect herbivores. Our review indicated the need for stringent protocols for surface sterilisation including thorough experimental controls. For molecular detection protocols by PCR, residual DNA from surface inocula must also be considered. The biocontrol potential of B. bassiana endophytes appears promising although both negative and neutral effects on insect herbivores were reported and there remains ambiguity with respect to the location and mode of action of the fungus in planta. We recommend that future studies adopt multiple techniques, including culture dependent and independent techniques for endophyte detection and elucidate the mechanisms involved against insect herbivores.
AB - In the last decade there has been increased focus on the potential of endophytic Beauveria bassiana for the biocontrol of insect herbivores. Generally, detection of endophytes is acknowledged to be problematic and recovery method-dependent. Herein, we critically analyse the methodology reported for the detection of B. bassiana as endophytes following experimental inoculation. In light of the methodology, we further review the effects of endophytic B. bassiana on insect herbivores. Our review indicated the need for stringent protocols for surface sterilisation including thorough experimental controls. For molecular detection protocols by PCR, residual DNA from surface inocula must also be considered. The biocontrol potential of B. bassiana endophytes appears promising although both negative and neutral effects on insect herbivores were reported and there remains ambiguity with respect to the location and mode of action of the fungus in planta. We recommend that future studies adopt multiple techniques, including culture dependent and independent techniques for endophyte detection and elucidate the mechanisms involved against insect herbivores.
KW - Cultivation-dependent
KW - Cultivation-independent detection
KW - Entomopathogenic fungi
KW - Herbivore
KW - Inoculation
KW - Insect
KW - Isolation
KW - Pest
KW - Surface disinfection
U2 - 10.1007/s10526-016-9769-5
DO - 10.1007/s10526-016-9769-5
M3 - Review
AN - SCOPUS:84991106505
SN - 1386-6141
VL - 62
SP - 1
EP - 17
JO - BioControl
JF - BioControl
IS - 1
ER -