Hypochlorite-induced oxidation of amino acids, peptides and proteins

TY - JOUR

T1 - Hypochlorite-induced oxidation of amino acids, peptides and proteins

AU - Hawkins, C L

AU - Pattison, D I

AU - Davies, Michael Jonathan

PY - 2003/12

Y1 - 2003/12

N2 - Activated phagocytes generate the potent oxidant hypochlorite (HOCl) via the release of the enzyme myeloperoxidase and hydrogen peroxide. HOCl is known to react with a number of biological targets including proteins, DNA, lipids and cholesterol. Proteins are likely to be major targets for reaction with HOCl within a cell due to their abundance and high reactivity with HOCl. This review summarizes information on the rate of reaction of HOCl with proteins, the nature of the intermediates formed, the mechanisms involved in protein oxidation and the products of these reactions. The predicted targets for reaction with HOCl from kinetic modeling studies and the consequences of HOCl-induced protein oxidation are also discussed.

AB - Activated phagocytes generate the potent oxidant hypochlorite (HOCl) via the release of the enzyme myeloperoxidase and hydrogen peroxide. HOCl is known to react with a number of biological targets including proteins, DNA, lipids and cholesterol. Proteins are likely to be major targets for reaction with HOCl within a cell due to their abundance and high reactivity with HOCl. This review summarizes information on the rate of reaction of HOCl with proteins, the nature of the intermediates formed, the mechanisms involved in protein oxidation and the products of these reactions. The predicted targets for reaction with HOCl from kinetic modeling studies and the consequences of HOCl-induced protein oxidation are also discussed.

KW - Amino Acids

KW - Catalysis

KW - Humans

KW - Hydrogen Peroxide

KW - Hypochlorous Acid

KW - Kinetics

KW - Oxidation-Reduction

KW - Peptides

KW - Peroxidase

KW - Proteins

U2 - 10.1007/s00726-003-0016-x

DO - 10.1007/s00726-003-0016-x

M3 - Journal article

C2 - 14661089

SN - 0939-4451

VL - 25

SP - 259

EP - 274

JO - Amino Acids

JF - Amino Acids

IS - 3-4

ER -