Hypersalinity as a trigger of seagrass (Thalassia testudinum) die-off events in Florida Bay: evidence based on shoot meristem O₂ and H₂S dynamics

TY - JOUR

T1 - Hypersalinity as a trigger of seagrass (Thalassia testudinum) die-off events in Florida Bay

T2 - evidence based on shoot meristem O2 and H2S dynamics

AU - Johnson, C. R.

AU - Koch, M. S.

AU - Pedersen, Ole

AU - Madden, C. J.

PY - 2018/7

Y1 - 2018/7

N2 - The mechanisms initiating rapid, large-scale (>50 km2) seagrass die-off events globally remain elusive. Thalassia testudinum, a dominant habitat-forming tropical seagrass of the Caribbean and tropical Atlantic region, experiences recurrent die-off events in Florida Bay. Thus, T. testudinum provides an excellent case study to identify triggers of large-scale seagrass mortality. Presently, seagrass die-off events in Florida Bay are correlated to high total sulfide (H2S, HS−, S2−) concentrations in the sediment porewater (≥2000 μM), water column hypoxia, high temperature and hypersalinity (salinity > 45). Because the mortality appears to be initiated at the shoot base within the meristem, we examined the response of shoot meristematic O2 and H2S dynamics using microsensors over a 5-hour nighttime simulation. Shoots were held at salinity 35 (ambient) or 65 (hypersaline) using microsensors in intact cores with plants from the field. The rate of H2S intrusion in the dark and oxidation in the light were similar at 35 and 65 salinity. However, the tissue O2 consumption rate was significantly higher under hypersaline conditions (−11.07 ± 4.32 kPa pO2 h−1) compared to ambient (−3.93 ± 0.88 kPa pO2 h−1) in the dark. Consequently, the meristematic pO2 threshold (~1.5 kPa O2) where H2S intrusion occurred in the meristem was reached more rapidly, increasing the time H2S accumulated (1.5 to 2.8 h). Longer H2S accumulation time significantly increased maximum meristem H2S levels at 65 (536 ± 330 μM H2S) compared to 35 salinity (121 ± 62 μM H2S). These results in T. testudinum provide evidence for a triggering mechanism that links an enhanced respiratory rate under hypersaline conditions to sulfide toxicity. We propose that hypersalinity in Florida Bay, or any stressor that significantly increases nighttime respiration rates, can subject seagrasses to longer and higher concentrations of sulfide, a known phytotoxin, within the shoot meristem. This mechanism likely explains large-scale mass mortality of T. testudinum in Florida Bay during periods of high salinity and elevated porewater sulfide levels, although field experiments are required to further validate this supposition.

AB - The mechanisms initiating rapid, large-scale (>50 km2) seagrass die-off events globally remain elusive. Thalassia testudinum, a dominant habitat-forming tropical seagrass of the Caribbean and tropical Atlantic region, experiences recurrent die-off events in Florida Bay. Thus, T. testudinum provides an excellent case study to identify triggers of large-scale seagrass mortality. Presently, seagrass die-off events in Florida Bay are correlated to high total sulfide (H2S, HS−, S2−) concentrations in the sediment porewater (≥2000 μM), water column hypoxia, high temperature and hypersalinity (salinity > 45). Because the mortality appears to be initiated at the shoot base within the meristem, we examined the response of shoot meristematic O2 and H2S dynamics using microsensors over a 5-hour nighttime simulation. Shoots were held at salinity 35 (ambient) or 65 (hypersaline) using microsensors in intact cores with plants from the field. The rate of H2S intrusion in the dark and oxidation in the light were similar at 35 and 65 salinity. However, the tissue O2 consumption rate was significantly higher under hypersaline conditions (−11.07 ± 4.32 kPa pO2 h−1) compared to ambient (−3.93 ± 0.88 kPa pO2 h−1) in the dark. Consequently, the meristematic pO2 threshold (~1.5 kPa O2) where H2S intrusion occurred in the meristem was reached more rapidly, increasing the time H2S accumulated (1.5 to 2.8 h). Longer H2S accumulation time significantly increased maximum meristem H2S levels at 65 (536 ± 330 μM H2S) compared to 35 salinity (121 ± 62 μM H2S). These results in T. testudinum provide evidence for a triggering mechanism that links an enhanced respiratory rate under hypersaline conditions to sulfide toxicity. We propose that hypersalinity in Florida Bay, or any stressor that significantly increases nighttime respiration rates, can subject seagrasses to longer and higher concentrations of sulfide, a known phytotoxin, within the shoot meristem. This mechanism likely explains large-scale mass mortality of T. testudinum in Florida Bay during periods of high salinity and elevated porewater sulfide levels, although field experiments are required to further validate this supposition.

U2 - 10.1016/j.jembe.2018.03.007

DO - 10.1016/j.jembe.2018.03.007

M3 - Journal article

SN - 0022-0981

VL - 504

SP - 47

EP - 52

JO - Journal of Experimental Marine Biology and Ecology

JF - Journal of Experimental Marine Biology and Ecology

ER -