Hyperbaric oxygen sensitizes anoxic Pseudomonas aeruginosa biofilm to ciprofloxacin

Mette Kolpen; Christian Johann Lerche; Kasper Nørskov Kragh; Thomas Sams; Klaus Koren; Anna S. Jensen; Laura Line; Thomas Bjarnsholt; Oana Ciofu; Claus Ernst Moser; Michael Kühl; Niels Høiby; Peter Østrup Jensen

doi:10.1128/AAC.01024-17

Hyperbaric oxygen sensitizes anoxic Pseudomonas aeruginosa biofilm to ciprofloxacin

Mette Kolpen, Christian Johann Lerche, Kasper Nørskov Kragh, Thomas Sams, Klaus Koren, Anna S. Jensen, Laura Line, Thomas Bjarnsholt, Oana Ciofu, Claus Ernst Moser, Michael Kühl, Niels Høiby, Peter Østrup Jensen

Marine Biological Section

19 Citationer (Scopus)

114 Downloads (Pure)

Abstract

Chronic Pseudomonas aeruginosa lung infection is characterized by the presence of endobronchial antibiotic-tolerant biofilm, which is subject to strong oxygen (O₂) depletion due to the activity of surrounding polymorphonuclear leukocytes. The exact mechanisms affecting the antibiotic susceptibility of biofilms remain unclear, but accumulating evidence suggests that the efficacy of several bactericidal antibiotics is enhanced by stimulation of aerobic respiration of pathogens, while lack of O₂ increases their tolerance. In fact, the bactericidal effect of several antibiotics depends on active aerobic metabolism activity and the endogenous formation of reactive O₂ radicals (ROS). In this study, we aimed to apply hyperbaric oxygen treatment (HBOT) to sensitize anoxic P. aeruginosa agarose biofilms established to mimic situations with intense O₂ consumption by the host response in the cystic fibrosis (CF) lung. Application of HBOT resulted in enhanced bactericidal activity of ciprofloxacin at clinically relevant durations and was accompanied by indications of restored aerobic respiration, involvement of endogenous lethal oxidative stress, and increased bacterial growth. The findings highlight that oxygenation by HBOT improves the bactericidal activity of ciprofloxacin on P.

Originalsprog	Engelsk
Artikelnummer	e01024-17
Tidsskrift	Antimicrobial Agents and Chemotherapy
Vol/bind	61
Udgave nummer	11
Antal sider	9
ISSN	0066-4804
DOI	https://doi.org/10.1128/AAC.01024-17
Status	Udgivet - nov. 2017

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10.1128/AAC.01024-17Licens: CC BY

Kolpen_2017_Hyperbaric_oxygenForlagets udgivne version, 1,56 MBLicens: CC BY

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title = "Hyperbaric oxygen sensitizes anoxic Pseudomonas aeruginosa biofilm to ciprofloxacin",

abstract = "Chronic Pseudomonas aeruginosa lung infection is characterized by the presence of endobronchial antibiotic-tolerant biofilm, which is subject to strong oxygen (O2) depletion due to the activity of surrounding polymorphonuclear leukocytes. The exact mechanisms affecting the antibiotic susceptibility of biofilms remain unclear, but accumulating evidence suggests that the efficacy of several bactericidal antibiotics is enhanced by stimulation of aerobic respiration of pathogens, while lack of O2 increases their tolerance. In fact, the bactericidal effect of several antibiotics depends on active aerobic metabolism activity and the endogenous formation of reactive O2 radicals (ROS). In this study, we aimed to apply hyperbaric oxygen treatment (HBOT) to sensitize anoxic P. aeruginosa agarose biofilms established to mimic situations with intense O2 consumption by the host response in the cystic fibrosis (CF) lung. Application of HBOT resulted in enhanced bactericidal activity of ciprofloxacin at clinically relevant durations and was accompanied by indications of restored aerobic respiration, involvement of endogenous lethal oxidative stress, and increased bacterial growth. The findings highlight that oxygenation by HBOT improves the bactericidal activity of ciprofloxacin on P.",

author = "Mette Kolpen and Lerche, {Christian Johann} and Kragh, {Kasper N{\o}rskov} and Thomas Sams and Klaus Koren and Jensen, {Anna S.} and Laura Line and Thomas Bjarnsholt and Oana Ciofu and Moser, {Claus Ernst} and Michael K{\"u}hl and Niels H{\o}iby and Jensen, {Peter {\O}strup}",

year = "2017",

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doi = "10.1128/AAC.01024-17",

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T1 - Hyperbaric oxygen sensitizes anoxic Pseudomonas aeruginosa biofilm to ciprofloxacin

AU - Kolpen, Mette

AU - Lerche, Christian Johann

AU - Kragh, Kasper Nørskov

AU - Sams, Thomas

AU - Koren, Klaus

AU - Jensen, Anna S.

AU - Line, Laura

AU - Bjarnsholt, Thomas

AU - Ciofu, Oana

AU - Moser, Claus Ernst

AU - Kühl, Michael

AU - Høiby, Niels

AU - Jensen, Peter Østrup

PY - 2017/11

Y1 - 2017/11

N2 - Chronic Pseudomonas aeruginosa lung infection is characterized by the presence of endobronchial antibiotic-tolerant biofilm, which is subject to strong oxygen (O2) depletion due to the activity of surrounding polymorphonuclear leukocytes. The exact mechanisms affecting the antibiotic susceptibility of biofilms remain unclear, but accumulating evidence suggests that the efficacy of several bactericidal antibiotics is enhanced by stimulation of aerobic respiration of pathogens, while lack of O2 increases their tolerance. In fact, the bactericidal effect of several antibiotics depends on active aerobic metabolism activity and the endogenous formation of reactive O2 radicals (ROS). In this study, we aimed to apply hyperbaric oxygen treatment (HBOT) to sensitize anoxic P. aeruginosa agarose biofilms established to mimic situations with intense O2 consumption by the host response in the cystic fibrosis (CF) lung. Application of HBOT resulted in enhanced bactericidal activity of ciprofloxacin at clinically relevant durations and was accompanied by indications of restored aerobic respiration, involvement of endogenous lethal oxidative stress, and increased bacterial growth. The findings highlight that oxygenation by HBOT improves the bactericidal activity of ciprofloxacin on P.

AB - Chronic Pseudomonas aeruginosa lung infection is characterized by the presence of endobronchial antibiotic-tolerant biofilm, which is subject to strong oxygen (O2) depletion due to the activity of surrounding polymorphonuclear leukocytes. The exact mechanisms affecting the antibiotic susceptibility of biofilms remain unclear, but accumulating evidence suggests that the efficacy of several bactericidal antibiotics is enhanced by stimulation of aerobic respiration of pathogens, while lack of O2 increases their tolerance. In fact, the bactericidal effect of several antibiotics depends on active aerobic metabolism activity and the endogenous formation of reactive O2 radicals (ROS). In this study, we aimed to apply hyperbaric oxygen treatment (HBOT) to sensitize anoxic P. aeruginosa agarose biofilms established to mimic situations with intense O2 consumption by the host response in the cystic fibrosis (CF) lung. Application of HBOT resulted in enhanced bactericidal activity of ciprofloxacin at clinically relevant durations and was accompanied by indications of restored aerobic respiration, involvement of endogenous lethal oxidative stress, and increased bacterial growth. The findings highlight that oxygenation by HBOT improves the bactericidal activity of ciprofloxacin on P.

U2 - 10.1128/AAC.01024-17

DO - 10.1128/AAC.01024-17

M3 - Journal article

C2 - 28874373

SN - 0066-4804

VL - 61

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

IS - 11

M1 - e01024-17

ER -

Hyperbaric oxygen sensitizes anoxic Pseudomonas aeruginosa biofilm to ciprofloxacin

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