Hybrid coating of alginate microbeads based on protein-biopolymer multilayers for encapsulation of probiotics

TY - JOUR

T1 - Hybrid coating of alginate microbeads based on protein-biopolymer multilayers for encapsulation of probiotics

AU - Yücel Falco, Cigdem

AU - Amadei, Federico

AU - Dhayal, Surender K.

AU - Cárdenas, Marité

AU - Tanaka, Motomu

AU - Risbo, Jens

PY - 2019/5/1

Y1 - 2019/5/1

N2 - A hybrid coating based on multilayers of proteins and biopolymers was developed to enhance the protection performance of alginate microbeads against acidic conditions for delivery of probiotics (Lactobacillus rhamnosus GG). Zeta potential measurements and quartz crystal microbalance with dissipation confirmed layer-by-layer deposition of protein-polymer layers. The stability of protein-based coatings during simulated gastric fluid (SGF) treatment was monitored by microscopy. Protein-coated microbeads were partially dismantled, whereas polymer-coated microbeads were intact after a sequential treatment in simulated gastric and intestinal fluids. This suggests that hybrid formulation offers an advantage over the coatings based on biopolymer multilayers in terms of better release of bacteria. Uncoated alginate microbeads completely dissolved and could not protect bacteria after SGF treatment whereas microbeads with hybrid coating showed increased physical stability and a modest decrease of culturability of 3.8 log units. Therefore, this work provides a concept for future protein-based hybrid coatings for bacterial delivery systems.

AB - A hybrid coating based on multilayers of proteins and biopolymers was developed to enhance the protection performance of alginate microbeads against acidic conditions for delivery of probiotics (Lactobacillus rhamnosus GG). Zeta potential measurements and quartz crystal microbalance with dissipation confirmed layer-by-layer deposition of protein-polymer layers. The stability of protein-based coatings during simulated gastric fluid (SGF) treatment was monitored by microscopy. Protein-coated microbeads were partially dismantled, whereas polymer-coated microbeads were intact after a sequential treatment in simulated gastric and intestinal fluids. This suggests that hybrid formulation offers an advantage over the coatings based on biopolymer multilayers in terms of better release of bacteria. Uncoated alginate microbeads completely dissolved and could not protect bacteria after SGF treatment whereas microbeads with hybrid coating showed increased physical stability and a modest decrease of culturability of 3.8 log units. Therefore, this work provides a concept for future protein-based hybrid coatings for bacterial delivery systems.

KW - alginate

KW - chitosan

KW - lactoferrin

KW - layer-by-layer

KW - microencapsulation of probiotics

U2 - 10.1002/btpr.2806

DO - 10.1002/btpr.2806

M3 - Journal article

C2 - 30884190

AN - SCOPUS:85063428235

SN - 8756-7938

VL - 35

JO - Biotechnology Progress

JF - Biotechnology Progress

IS - 3

M1 - e2806

ER -