How can we exploit functional genomics approaches for understanding the nature of plant defences? Barley as a case study

TY - CHAP

T1 - How can we exploit functional genomics approaches for understanding the nature of plant defences? Barley as a case study

AU - Collinge, David B.

AU - Jensen, Michael Krogh

AU - Lyngkjær, Michael Foged

AU - Rung, Jesper Henrik

PY - 2008

Y1 - 2008

N2 - The concept 'functional genomics' refers to the methods used for the functional characterisation of genomes. The methods utilised provide new opportunities for studying the nature and role of defence mechanisms in plants. Unlike Arabidopsis, poplar and rice, the full genomic sequence of barley is not available. In this case, the analysis of barley gene expression data plays a pivotal role for obtaining insight into the functional characterisation of individual gene products. Many genes are activated transcriptionally following attack by pathogens and these often contribute to the defence mechanisms which underlie disease resistance. The use of large-scale complementary DNA library constructions and genome-wide transcript profiles of plants exposed to biotic stress provide the data required to drive hypotheses concerning the function of newly identified genes. In this paper, we illustrate how publicly available gene expression data has proved valid for studies of plant defence responses; enabling a cost-effective workflow starting from isolated gene transcripts to elucidation of biological function upon biotic stress.

AB - The concept 'functional genomics' refers to the methods used for the functional characterisation of genomes. The methods utilised provide new opportunities for studying the nature and role of defence mechanisms in plants. Unlike Arabidopsis, poplar and rice, the full genomic sequence of barley is not available. In this case, the analysis of barley gene expression data plays a pivotal role for obtaining insight into the functional characterisation of individual gene products. Many genes are activated transcriptionally following attack by pathogens and these often contribute to the defence mechanisms which underlie disease resistance. The use of large-scale complementary DNA library constructions and genome-wide transcript profiles of plants exposed to biotic stress provide the data required to drive hypotheses concerning the function of newly identified genes. In this paper, we illustrate how publicly available gene expression data has proved valid for studies of plant defence responses; enabling a cost-effective workflow starting from isolated gene transcripts to elucidation of biological function upon biotic stress.

KW - Barley

KW - Blumeria graminis f.sp. hordei

KW - Functional genomics

KW - Hordeum vulgare

KW - NAC transcription factors

KW - Pathogen

KW - Plants

U2 - 10.1007/978-1-4020-8780-6_5

DO - 10.1007/978-1-4020-8780-6_5

M3 - Book chapter

AN - SCOPUS:84900888913

SN - 9781402087790

SP - 257

EP - 266

BT - Sustainable disease management in a European context

PB - Springer

ER -