Histology of Sculpin spp. in East Greenland. II. Histopathology and trace element concentrations

Henrik M. Kaarsholm; Ninna Verland; Rasmus D. Nørregaard; Lis Bach; Jens Søndergaard; Frank F. Rigét; Rune Dietz; Martin Hansen; Igor Eulaers; Jean Pierre Desforges; Pall S. Leifsson; Mai Dang; Barbara Nowak; Christian Sonne

doi:10.1080/02772248.2019.1579992

Histology of Sculpin spp. in East Greenland. II. Histopathology and trace element concentrations

Henrik M. Kaarsholm, Ninna Verland, Rasmus D. Nørregaard, Lis Bach, Jens Søndergaard, Frank F. Rigét, Rune Dietz, Martin Hansen, Igor Eulaers, Jean Pierre Desforges, Pall S. Leifsson, Mai Dang, Barbara Nowak, Christian Sonne^*

^*Corresponding author af dette arbejde

Abstract

For many years, the sculpin has been utilized as a sentinel monitoring species for anthropogenic impacts on the marine environment. To further develop its potential as a screening model body burden of several trace elements, including cadmium (Cd), copper (Cu), mercury (Hg), lead (Pb) and selenium (Se), were investigated. In addition, histopathology in shorthorn sculpins (Myoxocephalus scorpius; n = 20) and fourhorn sculpins (Myoxocephalus quadricornis; n = 10) were examined at three sites around the Scoresby Sound settlement in Central East Greenland. Fourhorn sculpins caught at the most distant site from the settlement, contained significantly higher hepatic Cu and Cd concentrations as well as significantly elevated gill Pb levels compared to the shorthorn sculpins collected at two other sites closer to the settlement. Histological examinations showed that fish with significantly higher concentrations of hepatic Cd and Se exhibited greater nuclear alterations, interstitial hyperplasia/hypertrophy, interstitial mononuclear infiltrations and granulomas. Further, fish with higher Cd and Cu gill tissue levels displayed a significantly higher number of cytoplasmic alterations and lamellar epithelium lifting, hypertrophic and hyperplastic epithelium along with mucus cell hyperplasia. While the presence of liver lesions were not species or sex-specific, the presence of gill lesions decreased in the order female fourhorn sculpins > female shorthorn sculpins > male fourhorn sculpins > male shorthorn sculpins. Hepatic Hg concentrations exceeded known lowest observable effect doses (LOED) for fish (0.1–0.5 μg g ⁻¹ ww) in 27% of fish, while liver Cd residues in 80% exceeded LOED (0.42–1.8 μg g ⁻¹ ww). Based upon these results, data suggest that using the sculpin as a valuable sentinel fish species histopathology may serve as a reliable tool for assessing marine ecosystem exposure to trace metals. However, confounding physiological and ecological factors also need to be considered.

Originalsprog	Engelsk
Tidsskrift	Toxicological and Environmental Chemistry
Vol/bind	100
Udgave nummer	8-10
Sider (fra-til)	769–784
ISSN	0277-2248
DOI	https://doi.org/10.1080/02772248.2019.1579992
Status	Udgivet - 26 nov. 2018

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10.1080/02772248.2019.1579992

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Kaarsholm, H. M., Verland, N., Nørregaard, R. D., Bach, L., Søndergaard, J., Rigét, F. F., Dietz, R., Hansen, M., Eulaers, I., Desforges, J. P., Leifsson, P. S., Dang, M., Nowak, B., & Sonne, C. (2018). Histology of Sculpin spp. in East Greenland. II. Histopathology and trace element concentrations. Toxicological and Environmental Chemistry, 100(8-10), 769–784. https://doi.org/10.1080/02772248.2019.1579992

Kaarsholm, HM, Verland, N, Nørregaard, RD, Bach, L, Søndergaard, J, Rigét, FF, Dietz, R, Hansen, M, Eulaers, I, Desforges, JP, Leifsson, PS, Dang, M, Nowak, B & Sonne, C 2018, 'Histology of Sculpin spp. in East Greenland. II. Histopathology and trace element concentrations', Toxicological and Environmental Chemistry, bind 100, nr. 8-10, s. 769–784. https://doi.org/10.1080/02772248.2019.1579992

@article{27aeb8efaedd4a7bbdf6f07f4237d42b,

title = "Histology of Sculpin spp. in East Greenland. II. Histopathology and trace element concentrations",

abstract = " For many years, the sculpin has been utilized as a sentinel monitoring species for anthropogenic impacts on the marine environment. To further develop its potential as a screening model body burden of several trace elements, including cadmium (Cd), copper (Cu), mercury (Hg), lead (Pb) and selenium (Se), were investigated. In addition, histopathology in shorthorn sculpins (Myoxocephalus scorpius; n = 20) and fourhorn sculpins (Myoxocephalus quadricornis; n = 10) were examined at three sites around the Scoresby Sound settlement in Central East Greenland. Fourhorn sculpins caught at the most distant site from the settlement, contained significantly higher hepatic Cu and Cd concentrations as well as significantly elevated gill Pb levels compared to the shorthorn sculpins collected at two other sites closer to the settlement. Histological examinations showed that fish with significantly higher concentrations of hepatic Cd and Se exhibited greater nuclear alterations, interstitial hyperplasia/hypertrophy, interstitial mononuclear infiltrations and granulomas. Further, fish with higher Cd and Cu gill tissue levels displayed a significantly higher number of cytoplasmic alterations and lamellar epithelium lifting, hypertrophic and hyperplastic epithelium along with mucus cell hyperplasia. While the presence of liver lesions were not species or sex-specific, the presence of gill lesions decreased in the order female fourhorn sculpins > female shorthorn sculpins > male fourhorn sculpins > male shorthorn sculpins. Hepatic Hg concentrations exceeded known lowest observable effect doses (LOED) for fish (0.1–0.5 μg g −1 ww) in 27% of fish, while liver Cd residues in 80% exceeded LOED (0.42–1.8 μg g −1 ww). Based upon these results, data suggest that using the sculpin as a valuable sentinel fish species histopathology may serve as a reliable tool for assessing marine ecosystem exposure to trace metals. However, confounding physiological and ecological factors also need to be considered. ",

keywords = "Arctic, Environmental monitoring, gills, liver, metals, selenium",

author = "Kaarsholm, {Henrik M.} and Ninna Verland and N{\o}rregaard, {Rasmus D.} and Lis Bach and Jens S{\o}ndergaard and Rig{\'e}t, {Frank F.} and Rune Dietz and Martin Hansen and Igor Eulaers and Desforges, {Jean Pierre} and Leifsson, {Pall S.} and Mai Dang and Barbara Nowak and Christian Sonne",

year = "2018",

month = nov,

day = "26",

doi = "10.1080/02772248.2019.1579992",

language = "English",

volume = "100",

pages = "769–784",

journal = "Toxicological and Environmental Chemistry",

issn = "0277-2248",

publisher = "Taylor & Francis",

number = "8-10",

}

TY - JOUR

T1 - Histology of Sculpin spp. in East Greenland. II. Histopathology and trace element concentrations

AU - Kaarsholm, Henrik M.

AU - Verland, Ninna

AU - Nørregaard, Rasmus D.

AU - Bach, Lis

AU - Søndergaard, Jens

AU - Rigét, Frank F.

AU - Dietz, Rune

AU - Hansen, Martin

AU - Eulaers, Igor

AU - Desforges, Jean Pierre

AU - Leifsson, Pall S.

AU - Dang, Mai

AU - Nowak, Barbara

AU - Sonne, Christian

PY - 2018/11/26

Y1 - 2018/11/26

N2 - For many years, the sculpin has been utilized as a sentinel monitoring species for anthropogenic impacts on the marine environment. To further develop its potential as a screening model body burden of several trace elements, including cadmium (Cd), copper (Cu), mercury (Hg), lead (Pb) and selenium (Se), were investigated. In addition, histopathology in shorthorn sculpins (Myoxocephalus scorpius; n = 20) and fourhorn sculpins (Myoxocephalus quadricornis; n = 10) were examined at three sites around the Scoresby Sound settlement in Central East Greenland. Fourhorn sculpins caught at the most distant site from the settlement, contained significantly higher hepatic Cu and Cd concentrations as well as significantly elevated gill Pb levels compared to the shorthorn sculpins collected at two other sites closer to the settlement. Histological examinations showed that fish with significantly higher concentrations of hepatic Cd and Se exhibited greater nuclear alterations, interstitial hyperplasia/hypertrophy, interstitial mononuclear infiltrations and granulomas. Further, fish with higher Cd and Cu gill tissue levels displayed a significantly higher number of cytoplasmic alterations and lamellar epithelium lifting, hypertrophic and hyperplastic epithelium along with mucus cell hyperplasia. While the presence of liver lesions were not species or sex-specific, the presence of gill lesions decreased in the order female fourhorn sculpins > female shorthorn sculpins > male fourhorn sculpins > male shorthorn sculpins. Hepatic Hg concentrations exceeded known lowest observable effect doses (LOED) for fish (0.1–0.5 μg g −1 ww) in 27% of fish, while liver Cd residues in 80% exceeded LOED (0.42–1.8 μg g −1 ww). Based upon these results, data suggest that using the sculpin as a valuable sentinel fish species histopathology may serve as a reliable tool for assessing marine ecosystem exposure to trace metals. However, confounding physiological and ecological factors also need to be considered.

AB - For many years, the sculpin has been utilized as a sentinel monitoring species for anthropogenic impacts on the marine environment. To further develop its potential as a screening model body burden of several trace elements, including cadmium (Cd), copper (Cu), mercury (Hg), lead (Pb) and selenium (Se), were investigated. In addition, histopathology in shorthorn sculpins (Myoxocephalus scorpius; n = 20) and fourhorn sculpins (Myoxocephalus quadricornis; n = 10) were examined at three sites around the Scoresby Sound settlement in Central East Greenland. Fourhorn sculpins caught at the most distant site from the settlement, contained significantly higher hepatic Cu and Cd concentrations as well as significantly elevated gill Pb levels compared to the shorthorn sculpins collected at two other sites closer to the settlement. Histological examinations showed that fish with significantly higher concentrations of hepatic Cd and Se exhibited greater nuclear alterations, interstitial hyperplasia/hypertrophy, interstitial mononuclear infiltrations and granulomas. Further, fish with higher Cd and Cu gill tissue levels displayed a significantly higher number of cytoplasmic alterations and lamellar epithelium lifting, hypertrophic and hyperplastic epithelium along with mucus cell hyperplasia. While the presence of liver lesions were not species or sex-specific, the presence of gill lesions decreased in the order female fourhorn sculpins > female shorthorn sculpins > male fourhorn sculpins > male shorthorn sculpins. Hepatic Hg concentrations exceeded known lowest observable effect doses (LOED) for fish (0.1–0.5 μg g −1 ww) in 27% of fish, while liver Cd residues in 80% exceeded LOED (0.42–1.8 μg g −1 ww). Based upon these results, data suggest that using the sculpin as a valuable sentinel fish species histopathology may serve as a reliable tool for assessing marine ecosystem exposure to trace metals. However, confounding physiological and ecological factors also need to be considered.

KW - Arctic

KW - Environmental monitoring

KW - gills

KW - liver

KW - metals

KW - selenium

U2 - 10.1080/02772248.2019.1579992

DO - 10.1080/02772248.2019.1579992

M3 - Journal article

AN - SCOPUS:85062514432

SN - 0277-2248

VL - 100

SP - 769

EP - 784

JO - Toxicological and Environmental Chemistry

JF - Toxicological and Environmental Chemistry

IS - 8-10

ER -

Histology of Sculpin spp. in East Greenland. II. Histopathology and trace element concentrations

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