Histological evaluation of vertical laser channels from ablative fractional resurfacing: an ex vivo pig skin model

Christina Skovbølling Haak, Monica Illes, Uwe Paasch, Merete Hædersdal

39 Citationer (Scopus)

Abstract

Ablative fractional resurfacing (AFR) represents a new treatment potential for various skin conditions and new laser devices are being introduced. It is important to gain information about the impact of laser settings on the dimensions of the created laser channels for obtaining a safe and efficient treatment outcome. The aim of this study was to establish a standard model to document the histological tissue damage profiles after AFR and to test a new laser device at diverse settings. Ex vivo abdominal pig skin was treated with a MedArt 620, prototype fractional carbon dioxide (CO(2)) laser (Medart, Hvidovre, Denmark) delivering single microbeams (MB) with a spot size of 165 µm. By using a constant pulse duration of 2 ms, intensities of 1-18 W, single and 2-4 stacked pulses, energies were delivered in a range from 2-144 mJ/MB. Histological evaluations included 3-4 high-quality histological measurements for each laser setting (n¿=¿28). AFR created cone-shaped laser channels. Ablation depths varied from reaching the superficial dermis (2 mJ, median 41 µm) to approaching the subcutaneous fat (144 mJ, median 1,943 µm) and correlated to the applied energy levels in an approximate linear relation (r(2)¿=¿0.84, p¿
OriginalsprogEngelsk
TidsskriftLasers in Medical Science
Vol/bind26
Udgave nummer4
Sider (fra-til)465-71
Antal sider7
ISSN0268-8921
DOI
StatusUdgivet - jul. 2011

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