Highly efficient method for isolation of total RNA from adipose tissue

TY - JOUR

T1 - Highly efficient method for isolation of total RNA from adipose tissue

AU - Cirera Salicio, Susanna

PY - 2013/11/18

Y1 - 2013/11/18

N2 - Background: RNA extraction is a crucial step for monitoring gene expression. Poor RNA quality (including degradation and remaining impurities) can result in misleading results. Isolation of RNA from animal tissues with high lipid content can be challenging. Especially, it is not trivial to isolate high quality RNA with a reasonable yield from adipose tissue. The aim of this study was to provide an optimized protocol for isolating total RNA from adipose tissue. This was achieved by combining the advantages of the two routinely used methods, TRI Reagent® and miRNeasy. Findings. The miRNeasy method results in cleaner samples but more prone to degradation while the TRI Reagent® method results in samples contaminated with salts and solvents but more intact. The new protocol combines the best of both methods resulting in RNA of high quality and suitable for downstream experiments like RT-qPCR, microarrays and high-throughput sequencing. Conclusions: The current protocol for total RNA isolation from adipose tissue yields sufficient amount of high quality total RNA free of contaminants.

AB - Background: RNA extraction is a crucial step for monitoring gene expression. Poor RNA quality (including degradation and remaining impurities) can result in misleading results. Isolation of RNA from animal tissues with high lipid content can be challenging. Especially, it is not trivial to isolate high quality RNA with a reasonable yield from adipose tissue. The aim of this study was to provide an optimized protocol for isolating total RNA from adipose tissue. This was achieved by combining the advantages of the two routinely used methods, TRI Reagent® and miRNeasy. Findings. The miRNeasy method results in cleaner samples but more prone to degradation while the TRI Reagent® method results in samples contaminated with salts and solvents but more intact. The new protocol combines the best of both methods resulting in RNA of high quality and suitable for downstream experiments like RT-qPCR, microarrays and high-throughput sequencing. Conclusions: The current protocol for total RNA isolation from adipose tissue yields sufficient amount of high quality total RNA free of contaminants.

UR - http://www.scopus.com/inward/record.url?scp=84887754659&partnerID=8YFLogxK

U2 - 10.1186/1756-0500-6-472

DO - 10.1186/1756-0500-6-472

M3 - Journal article

C2 - 24245791

AN - SCOPUS:84887754659

SN - 1756-0500

VL - 6

JO - BMC Research Notes

JF - BMC Research Notes

M1 - 472

ER -