@article{1e1c8a6c02844a5b83180bfc7b4f15f2,
title = "Higher-energy C-trap dissociation for peptide modification analysis",
abstract = "Peptide sequencing is the basis of mass spectrometry-driven proteomics. Here we show that in the linear ion trap-orbitrap mass spectrometer (LTQ Orbitrap) peptide ions can be efficiently fragmented by high-accuracy and full-mass-range tandem mass spectrometry (MS/MS) via higher-energy C-trap dissociation (HCD). Immonium ions generated via HCD pinpoint modifications such as phosphotyrosine with very high confidence. Additionally we show that an added octopole collision cell facilitates de novo sequencing.",
author = "Olsen, {Jesper Velgaard} and Boris Macek and Oliver Lange and Alexander Makarov and Stevan Horning and Matthias Mann",
note = "Keywords: Mass Spectrometry; Peptide Fragments; Protein Conformation; Proteomics; Tandem Mass Spectrometry",
year = "2007",
doi = "10.1038/nmeth1060",
language = "English",
volume = "4",
pages = "709--12",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "nature publishing group",
number = "9",
}