@inbook{9ac3419bd5da4841aa0532d150e8e24e,
title = "High-throughput siRNA screening applied to the ubiquitin-proteasome system",
abstract = "The ubiquitin-proteasome system is the major pathway for intracellular protein degradation in eukaryotic cells. Due to the large number of genes dedicated to the ubiquitin-proteasome system, mapping degradation pathways for short lived proteins is a daunting task, in particular in mammalian cells that are not genetically tractable as, for instance, a yeast model system. Here, we describe a method relying on high-throughput cellular imaging of cells transfected with a targeted siRNA library to screen for components involved in degradation of a protein of interest. This method is a rapid and cost-effective tool which is also highly applicable for other studies on gene function.",
keywords = "Journal Article",
author = "Poulsen, {Esben Guldahl} and Nielsen, {Sofie V.} and Pietras, {Elin J.} and Johansen, {Jens Vilstrup} and Cornelia Steinhauer and Rasmus Hartmann-Petersen",
year = "2016",
month = sep,
day = "1",
doi = "10.1007/978-1-4939-3756-1_28",
language = "English",
isbn = "978-1-4939-3754-7",
series = "Methods in Molecular Biology",
publisher = "Springer",
pages = "421--439",
editor = "Rune Matthiesen",
booktitle = "Proteostasis",
}