High resolution DNA flow cytometry of boar sperm cells in identification of boars carrying cytogenetic aberrations

TY - JOUR

T1 - High resolution DNA flow cytometry of boar sperm cells in identification of boars carrying cytogenetic aberrations

AU - Larsen, Jacob

AU - Christensen, Knud

AU - Larsen, Jørgen K

AU - Jensen, Peter Østrup

AU - Gustavsson, Ingemar

AU - Christensen, Ib Jarle

PY - 2004/8

Y1 - 2004/8

N2 - The cytogenetic quality of boars used for breeding determines the litter outcome and thus has large economical consequences. Traditionally, quality controls based on the examination of simple karyograms are time consuming and sometimes give uncertain results. As an alternative, the use of high-resolution DNA flow cytometry on DAPI-stained sperm cell nuclei (CV </= 1.3%) was investigated as a screening method for the cytogenetic quality of boars. By analyzing a series of 25 animals judged normal by their fertility statistics and a series of seven animals with known reciprocal translocations, a model for identifying sperm cells from cytogenetically aberrant animals was proposed. This model was applied to a series of 50 uncharacterized animals. The model successfully identified a mosaic or chimaeric carrier of an aberrant X chromosome. However, implementation of this technique for screening purposes would necessitate essential improvements in standardization and measurement precision.

AB - The cytogenetic quality of boars used for breeding determines the litter outcome and thus has large economical consequences. Traditionally, quality controls based on the examination of simple karyograms are time consuming and sometimes give uncertain results. As an alternative, the use of high-resolution DNA flow cytometry on DAPI-stained sperm cell nuclei (CV </= 1.3%) was investigated as a screening method for the cytogenetic quality of boars. By analyzing a series of 25 animals judged normal by their fertility statistics and a series of seven animals with known reciprocal translocations, a model for identifying sperm cells from cytogenetically aberrant animals was proposed. This model was applied to a series of 50 uncharacterized animals. The model successfully identified a mosaic or chimaeric carrier of an aberrant X chromosome. However, implementation of this technique for screening purposes would necessitate essential improvements in standardization and measurement precision.

KW - Animals

KW - Chromosome Aberrations

KW - DNA

KW - Flow Cytometry

KW - Fluorescent Dyes

KW - Indoles

KW - Karyotyping

KW - Male

KW - Spermatozoa

KW - Swine

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/j.theriogenology.2003.11.008

DO - 10.1016/j.theriogenology.2003.11.008

M3 - Journal article

C2 - 15226006

SN - 0093-691X

VL - 62

SP - 501

EP - 511

JO - Theriogenology

JF - Theriogenology

IS - 3-4

ER -