Heterologous production of the widely used natural food colorant carminic acid in Aspergillus nidulans

Rasmus J.N. Frandsen; Paiman Khorsand-Jamal; Kenneth Thermann Kongstad; Majse Nafisi; Rubini Maya Kannangara; Dan Stærk; Finn T. Okkels; Kim Binderup; Bjørn Madsen; Birger Lindberg Møller; Ulf Thrane; Uffe H. Mortensen

doi:10.1038/s41598-018-30816-9

Heterologous production of the widely used natural food colorant carminic acid in Aspergillus nidulans

Rasmus J.N. Frandsen, Paiman Khorsand-Jamal, Kenneth Thermann Kongstad, Majse Nafisi, Rubini Maya Kannangara, Dan Stærk, Finn T. Okkels, Kim Binderup, Bjørn Madsen, Birger Lindberg Møller, Ulf Thrane, Uffe H. Mortensen

Plantebiokemi

15 Citationer (Scopus)

82 Downloads (Pure)

Abstract

The natural red food colorants carmine (E120) and carminic acid are currently produced from scale insects. The access to raw material is limited and current production is sensitive to fluctuation in weather conditions. A cheaper and more stable supply is therefore desirable. Here we present the first proof-of-concept of heterologous microbial production of carminic acid in Aspergillus nidulans by developing a semi-natural biosynthetic pathway. Formation of the tricyclic core of carminic acid is achieved via a two-step process wherein a plant type III polyketide synthase (PKS) forms a non-reduced linear octaketide, which subsequently is folded into the desired flavokermesic acid anthrone (FKA) structure by a cyclase and a aromatase from a bacterial type II PKS system. The formed FKA is oxidized to flavokermesic acid and kermesic acid, catalyzed by endogenous A. nidulans monooxygenases, and further converted to dcII and carminic acid by the Dactylopius coccus C-glucosyltransferase DcUGT2. The establishment of a functional biosynthetic carminic acid pathway in A. nidulans serves as an important step towards industrial-scale production of carminic acid via liquid-state fermentation using a microbial cell factory.

Originalsprog	Engelsk
Artikelnummer	12853
Tidsskrift	Scientific Reports
Vol/bind	8
Sider (fra-til)	1-10
Antal sider	10
ISSN	2045-2322
DOI	https://doi.org/10.1038/s41598-018-30816-9
Status	Udgivet - 1 dec. 2018

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10.1038/s41598-018-30816-9

Heterologous production of the widely used natural food colorant carminic acid in Aspergillus nidulansForlagets udgivne version, 4,28 MB

Citationsformater

Frandsen, R. J. N., Khorsand-Jamal, P., Kongstad, K. T., Nafisi, M., Kannangara, R. M., Stærk, D., Okkels, F. T., Binderup, K., Madsen, B., Møller, B. L., Thrane, U., & Mortensen, U. H. (2018). Heterologous production of the widely used natural food colorant carminic acid in Aspergillus nidulans. Scientific Reports, 8, 1-10. [12853]. https://doi.org/10.1038/s41598-018-30816-9

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abstract = "The natural red food colorants carmine (E120) and carminic acid are currently produced from scale insects. The access to raw material is limited and current production is sensitive to fluctuation in weather conditions. A cheaper and more stable supply is therefore desirable. Here we present the first proof-of-concept of heterologous microbial production of carminic acid in Aspergillus nidulans by developing a semi-natural biosynthetic pathway. Formation of the tricyclic core of carminic acid is achieved via a two-step process wherein a plant type III polyketide synthase (PKS) forms a non-reduced linear octaketide, which subsequently is folded into the desired flavokermesic acid anthrone (FKA) structure by a cyclase and a aromatase from a bacterial type II PKS system. The formed FKA is oxidized to flavokermesic acid and kermesic acid, catalyzed by endogenous A. nidulans monooxygenases, and further converted to dcII and carminic acid by the Dactylopius coccus C-glucosyltransferase DcUGT2. The establishment of a functional biosynthetic carminic acid pathway in A. nidulans serves as an important step towards industrial-scale production of carminic acid via liquid-state fermentation using a microbial cell factory.",

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AU - Frandsen, Rasmus J.N.

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AU - Kongstad, Kenneth Thermann

AU - Nafisi, Majse

AU - Kannangara, Rubini Maya

AU - Stærk, Dan

AU - Okkels, Finn T.

AU - Binderup, Kim

AU - Madsen, Bjørn

AU - Møller, Birger Lindberg

AU - Thrane, Ulf

AU - Mortensen, Uffe H.

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N2 - The natural red food colorants carmine (E120) and carminic acid are currently produced from scale insects. The access to raw material is limited and current production is sensitive to fluctuation in weather conditions. A cheaper and more stable supply is therefore desirable. Here we present the first proof-of-concept of heterologous microbial production of carminic acid in Aspergillus nidulans by developing a semi-natural biosynthetic pathway. Formation of the tricyclic core of carminic acid is achieved via a two-step process wherein a plant type III polyketide synthase (PKS) forms a non-reduced linear octaketide, which subsequently is folded into the desired flavokermesic acid anthrone (FKA) structure by a cyclase and a aromatase from a bacterial type II PKS system. The formed FKA is oxidized to flavokermesic acid and kermesic acid, catalyzed by endogenous A. nidulans monooxygenases, and further converted to dcII and carminic acid by the Dactylopius coccus C-glucosyltransferase DcUGT2. The establishment of a functional biosynthetic carminic acid pathway in A. nidulans serves as an important step towards industrial-scale production of carminic acid via liquid-state fermentation using a microbial cell factory.

AB - The natural red food colorants carmine (E120) and carminic acid are currently produced from scale insects. The access to raw material is limited and current production is sensitive to fluctuation in weather conditions. A cheaper and more stable supply is therefore desirable. Here we present the first proof-of-concept of heterologous microbial production of carminic acid in Aspergillus nidulans by developing a semi-natural biosynthetic pathway. Formation of the tricyclic core of carminic acid is achieved via a two-step process wherein a plant type III polyketide synthase (PKS) forms a non-reduced linear octaketide, which subsequently is folded into the desired flavokermesic acid anthrone (FKA) structure by a cyclase and a aromatase from a bacterial type II PKS system. The formed FKA is oxidized to flavokermesic acid and kermesic acid, catalyzed by endogenous A. nidulans monooxygenases, and further converted to dcII and carminic acid by the Dactylopius coccus C-glucosyltransferase DcUGT2. The establishment of a functional biosynthetic carminic acid pathway in A. nidulans serves as an important step towards industrial-scale production of carminic acid via liquid-state fermentation using a microbial cell factory.

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ER -

Heterologous production of the widely used natural food colorant carminic acid in Aspergillus nidulans

Abstract

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