Healthy Nordic diet modulates the expression of genes related to mitochondrial function and immune response in peripheral blood mononuclear cells from subjects with the metabolic syndrome - a SYSDIET sub-study

Mari C W Myhrstad; Vanessa D de Mello; Ingrid Dahlman; Marjukka Kolehmainen; Jussi Paananen; Amanda Rundblad; Carsten Carlberg; Ole Kristoffer Olstad; Jussi Pihlajamäki; Kirsten B Holven; Kjeld Hermansen; Lars Ove Dragsted; Ingibjörg Gunnarsdottir; Lieselotte Cloetens; Matilda Ulmius Storm; Björn Åkesson; Fredrik Rosqvist; Janne Hukkanen; Karl-Heinz Herzig; Ulf Risérus; Inga Thorsdottir; Kaisa S Poutanen; Markku J Savolainen; Ursula Schwab; Peter Arner; Matti Uusitupa; Stine M Ulven

doi:10.1002/mnfr.201801405

Healthy Nordic diet modulates the expression of genes related to mitochondrial function and immune response in peripheral blood mononuclear cells from subjects with the metabolic syndrome - a SYSDIET sub-study

Mari C W Myhrstad, Vanessa D de Mello, Ingrid Dahlman, Marjukka Kolehmainen, Jussi Paananen, Amanda Rundblad, Carsten Carlberg, Ole Kristoffer Olstad, Jussi Pihlajamäki, Kirsten B Holven, Kjeld Hermansen, Lars Ove Dragsted, Ingibjörg Gunnarsdottir, Lieselotte Cloetens, Matilda Ulmius Storm, Björn Åkesson, Fredrik Rosqvist, Janne Hukkanen, Karl-Heinz Herzig, Ulf RisérusInga Thorsdottir, Kaisa S Poutanen, Markku J Savolainen, Ursula Schwab, Peter Arner, Matti Uusitupa, Stine M Ulven

Ernæring og Sundhed

6 Citationer (Scopus)

Abstract

Scope: To explore the effect of a healthy Nordic diet on the global transcriptome profile in peripheral blood mononuclear cells (PBMCs) of subjects with metabolic syndrome. Methods and results: Subjects with metabolic syndrome undergo a 18/24 week randomized intervention study comparing an isocaloric healthy Nordic diet with an average habitual Nordic diet served as control (SYSDIET study). Altogether, 68 participants are included. PBMCs are obtained before and after intervention and total RNA is subjected to global transcriptome analysis. 1302 probe sets are differentially expressed between the diet groups (p-value < 0.05). Twenty-five of these are significantly regulated (FDR q-value < 0.25) and are mainly involved in mitochondrial function, cell growth, and cell adhesion. The list of 1302 regulated probe sets is subjected to functional analyses. Pathways and processes involved in the mitochondrial electron transport chain, immune response, and cell cycle are downregulated in the healthy Nordic diet group. In addition, gene transcripts with common motifs for 42 transcription factors, including NFR1, NFR2, and NF-κB, are downregulated in the healthy Nordic diet group. Conclusion: These results suggest that benefits of a healthy diet may be mediated by improved mitochondrial function and reduced inflammation.

Originalsprog	Engelsk
Artikelnummer	1801405
Tidsskrift	Molecular Nutrition & Food Research
Vol/bind	63
Udgave nummer	13
Antal sider	13
ISSN	1613-4125
DOI	https://doi.org/10.1002/mnfr.201801405
Status	Udgivet - jul. 2019

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Det Natur- og Biovidenskabelige Fakultet

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10.1002/mnfr.201801405

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Citationsformater

Myhrstad, M. C. W., de Mello, V. D., Dahlman, I., Kolehmainen, M., Paananen, J., Rundblad, A., Carlberg, C., Olstad, O. K., Pihlajamäki, J., Holven, K. B., Hermansen, K., Dragsted, L. O., Gunnarsdottir, I., Cloetens, L., Storm, M. U., Åkesson, B., Rosqvist, F., Hukkanen, J., Herzig, K-H., ... Ulven, S. M. (2019). Healthy Nordic diet modulates the expression of genes related to mitochondrial function and immune response in peripheral blood mononuclear cells from subjects with the metabolic syndrome - a SYSDIET sub-study. Molecular Nutrition & Food Research, 63(13), [1801405]. https://doi.org/10.1002/mnfr.201801405

Healthy Nordic diet modulates the expression of genes related to mitochondrial function and immune response in peripheral blood mononuclear cells from subjects with the metabolic syndrome - a SYSDIET sub-study. / Myhrstad, Mari C W; de Mello, Vanessa D; Dahlman, Ingrid et al.

I: Molecular Nutrition & Food Research, Bind 63, Nr. 13, 1801405, 07.2019.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Myhrstad, MCW, de Mello, VD, Dahlman, I, Kolehmainen, M, Paananen, J, Rundblad, A, Carlberg, C, Olstad, OK, Pihlajamäki, J, Holven, KB, Hermansen, K, Dragsted, LO, Gunnarsdottir, I, Cloetens, L, Storm, MU, Åkesson, B, Rosqvist, F, Hukkanen, J, Herzig, K-H, Risérus, U, Thorsdottir, I, Poutanen, KS, Savolainen, MJ, Schwab, U, Arner, P, Uusitupa, M & Ulven, SM 2019, 'Healthy Nordic diet modulates the expression of genes related to mitochondrial function and immune response in peripheral blood mononuclear cells from subjects with the metabolic syndrome - a SYSDIET sub-study', Molecular Nutrition & Food Research, bind 63, nr. 13, 1801405. https://doi.org/10.1002/mnfr.201801405

Myhrstad MCW, de Mello VD, Dahlman I, Kolehmainen M, Paananen J, Rundblad A et al. Healthy Nordic diet modulates the expression of genes related to mitochondrial function and immune response in peripheral blood mononuclear cells from subjects with the metabolic syndrome - a SYSDIET sub-study. Molecular Nutrition & Food Research. 2019 jul.;63(13):1801405. doi: 10.1002/mnfr.201801405

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title = "Healthy Nordic diet modulates the expression of genes related to mitochondrial function and immune response in peripheral blood mononuclear cells from subjects with the metabolic syndrome - a SYSDIET sub-study",

abstract = "Scope: To explore the effect of a healthy Nordic diet on the global transcriptome profile in peripheral blood mononuclear cells (PBMCs) of subjects with metabolic syndrome. Methods and results: Subjects with metabolic syndrome undergo a 18/24 week randomized intervention study comparing an isocaloric healthy Nordic diet with an average habitual Nordic diet served as control (SYSDIET study). Altogether, 68 participants are included. PBMCs are obtained before and after intervention and total RNA is subjected to global transcriptome analysis. 1302 probe sets are differentially expressed between the diet groups (p-value < 0.05). Twenty-five of these are significantly regulated (FDR q-value < 0.25) and are mainly involved in mitochondrial function, cell growth, and cell adhesion. The list of 1302 regulated probe sets is subjected to functional analyses. Pathways and processes involved in the mitochondrial electron transport chain, immune response, and cell cycle are downregulated in the healthy Nordic diet group. In addition, gene transcripts with common motifs for 42 transcription factors, including NFR1, NFR2, and NF-κB, are downregulated in the healthy Nordic diet group. Conclusion: These results suggest that benefits of a healthy diet may be mediated by improved mitochondrial function and reduced inflammation.",

keywords = "Faculty of Science, Healthy Nordic diet, Metabolic syndrome, PBMCs, Transcriptome, Gene-expression",

author = "Myhrstad, {Mari C W} and {de Mello}, {Vanessa D} and Ingrid Dahlman and Marjukka Kolehmainen and Jussi Paananen and Amanda Rundblad and Carsten Carlberg and Olstad, {Ole Kristoffer} and Jussi Pihlajam{\"a}ki and Holven, {Kirsten B} and Kjeld Hermansen and Dragsted, {Lars Ove} and Ingibj{\"o}rg Gunnarsdottir and Lieselotte Cloetens and Storm, {Matilda Ulmius} and Bj{\"o}rn {\AA}kesson and Fredrik Rosqvist and Janne Hukkanen and Karl-Heinz Herzig and Ulf Ris{\'e}rus and Inga Thorsdottir and Poutanen, {Kaisa S} and Savolainen, {Markku J} and Ursula Schwab and Peter Arner and Matti Uusitupa and Ulven, {Stine M}",

year = "2019",

month = jul,

doi = "10.1002/mnfr.201801405",

language = "English",

volume = "63",

journal = "Molecular Nutrition & Food Research",

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T1 - Healthy Nordic diet modulates the expression of genes related to mitochondrial function and immune response in peripheral blood mononuclear cells from subjects with the metabolic syndrome - a SYSDIET sub-study

AU - Myhrstad, Mari C W

AU - de Mello, Vanessa D

AU - Dahlman, Ingrid

AU - Kolehmainen, Marjukka

AU - Paananen, Jussi

AU - Rundblad, Amanda

AU - Carlberg, Carsten

AU - Olstad, Ole Kristoffer

AU - Pihlajamäki, Jussi

AU - Holven, Kirsten B

AU - Hermansen, Kjeld

AU - Dragsted, Lars Ove

AU - Gunnarsdottir, Ingibjörg

AU - Cloetens, Lieselotte

AU - Storm, Matilda Ulmius

AU - Åkesson, Björn

AU - Rosqvist, Fredrik

AU - Hukkanen, Janne

AU - Herzig, Karl-Heinz

AU - Risérus, Ulf

AU - Thorsdottir, Inga

AU - Poutanen, Kaisa S

AU - Savolainen, Markku J

AU - Schwab, Ursula

AU - Arner, Peter

AU - Uusitupa, Matti

AU - Ulven, Stine M

PY - 2019/7

Y1 - 2019/7

N2 - Scope: To explore the effect of a healthy Nordic diet on the global transcriptome profile in peripheral blood mononuclear cells (PBMCs) of subjects with metabolic syndrome. Methods and results: Subjects with metabolic syndrome undergo a 18/24 week randomized intervention study comparing an isocaloric healthy Nordic diet with an average habitual Nordic diet served as control (SYSDIET study). Altogether, 68 participants are included. PBMCs are obtained before and after intervention and total RNA is subjected to global transcriptome analysis. 1302 probe sets are differentially expressed between the diet groups (p-value < 0.05). Twenty-five of these are significantly regulated (FDR q-value < 0.25) and are mainly involved in mitochondrial function, cell growth, and cell adhesion. The list of 1302 regulated probe sets is subjected to functional analyses. Pathways and processes involved in the mitochondrial electron transport chain, immune response, and cell cycle are downregulated in the healthy Nordic diet group. In addition, gene transcripts with common motifs for 42 transcription factors, including NFR1, NFR2, and NF-κB, are downregulated in the healthy Nordic diet group. Conclusion: These results suggest that benefits of a healthy diet may be mediated by improved mitochondrial function and reduced inflammation.

AB - Scope: To explore the effect of a healthy Nordic diet on the global transcriptome profile in peripheral blood mononuclear cells (PBMCs) of subjects with metabolic syndrome. Methods and results: Subjects with metabolic syndrome undergo a 18/24 week randomized intervention study comparing an isocaloric healthy Nordic diet with an average habitual Nordic diet served as control (SYSDIET study). Altogether, 68 participants are included. PBMCs are obtained before and after intervention and total RNA is subjected to global transcriptome analysis. 1302 probe sets are differentially expressed between the diet groups (p-value < 0.05). Twenty-five of these are significantly regulated (FDR q-value < 0.25) and are mainly involved in mitochondrial function, cell growth, and cell adhesion. The list of 1302 regulated probe sets is subjected to functional analyses. Pathways and processes involved in the mitochondrial electron transport chain, immune response, and cell cycle are downregulated in the healthy Nordic diet group. In addition, gene transcripts with common motifs for 42 transcription factors, including NFR1, NFR2, and NF-κB, are downregulated in the healthy Nordic diet group. Conclusion: These results suggest that benefits of a healthy diet may be mediated by improved mitochondrial function and reduced inflammation.

KW - Faculty of Science

KW - Healthy Nordic diet

KW - Metabolic syndrome

KW - PBMCs

KW - Transcriptome

KW - Gene-expression

U2 - 10.1002/mnfr.201801405

DO - 10.1002/mnfr.201801405

M3 - Journal article

C2 - 30964598

SN - 1613-4125

VL - 63

JO - Molecular Nutrition & Food Research

JF - Molecular Nutrition & Food Research

IS - 13

M1 - 1801405

ER -