HD-GYP domain proteins regulate biofilm formation and virulence in Pseudomonas aeruginosa

TY - JOUR

T1 - HD-GYP domain proteins regulate biofilm formation and virulence in Pseudomonas aeruginosa

AU - Ryan, Robert P

AU - Lucey, Jean

AU - O'Donovan, Karen

AU - McCarthy, Yvonne

AU - Yang, Liang

AU - Tolker-Nielsen, Tim

AU - Dow, J Maxwell

N1 - Keywords: 3',5'-Cyclic-GMP Phosphodiesterases; ADP Ribose Transferases; Animals; Bacterial Proteins; Bacterial Toxins; Biofilms; Cyclic GMP; Cytoplasm; Gene Expression Regulation, Bacterial; Gene Knockout Techniques; Genes, Bacterial; Larva; Lepidoptera; Locomotion; Oligopeptides; Pseudomonas Infections; Pseudomonas aeruginosa; Pyocyanine; Survival Analysis; Virulence; Virulence Factors

PY - 2009

Y1 - 2009

N2 - HD-GYP is a protein domain involved in the hydrolysis of the bacterial second messenger cyclic-di-GMP. The genome of the human pathogen Pseudomonas aeruginosa PAO1 encodes two proteins (PA4108, PA4781) with an HD-GYP domain and a third protein, PA2572, which contains a domain with variant key residues (YN-GYP). Here we have investigated the role of these proteins in biofilm formation, virulence factor synthesis and virulence of P. aeruginosa. Mutation of PA4108 and PA4781 led to an increase in the level of cyclic-di-GMP in P. aeruginosa, consistent with the predicted activity of the encoded proteins as cyclic-di-GMP phosphodiesterases. Mutation of both genes led to reduced swarming motility but had differing effects on production of the virulence factors pyocyanin, pyoverdin and ExoS. Mutation of PA2572 had no effect on cyclic-di-GMP levels and did not influence swarming motility. However, PA2572 had a negative influence on swarming that was cryptic and was revealed only after removal of an uncharacterized C-terminal domain. Mutation of PA4108, PA4781 and PA2572 had distinct effects on biofilm formation and architecture of P. aeruginosa. All three proteins contributed to virulence of P. aeruginosa to larvae of the Greater Wax moth Galleria mellonella.

AB - HD-GYP is a protein domain involved in the hydrolysis of the bacterial second messenger cyclic-di-GMP. The genome of the human pathogen Pseudomonas aeruginosa PAO1 encodes two proteins (PA4108, PA4781) with an HD-GYP domain and a third protein, PA2572, which contains a domain with variant key residues (YN-GYP). Here we have investigated the role of these proteins in biofilm formation, virulence factor synthesis and virulence of P. aeruginosa. Mutation of PA4108 and PA4781 led to an increase in the level of cyclic-di-GMP in P. aeruginosa, consistent with the predicted activity of the encoded proteins as cyclic-di-GMP phosphodiesterases. Mutation of both genes led to reduced swarming motility but had differing effects on production of the virulence factors pyocyanin, pyoverdin and ExoS. Mutation of PA2572 had no effect on cyclic-di-GMP levels and did not influence swarming motility. However, PA2572 had a negative influence on swarming that was cryptic and was revealed only after removal of an uncharacterized C-terminal domain. Mutation of PA4108, PA4781 and PA2572 had distinct effects on biofilm formation and architecture of P. aeruginosa. All three proteins contributed to virulence of P. aeruginosa to larvae of the Greater Wax moth Galleria mellonella.

U2 - 10.1111/j.1462-2920.2008.01842.x

DO - 10.1111/j.1462-2920.2008.01842.x

M3 - Journal article

C2 - 19170727

SN - 1462-2912

VL - 11

SP - 1126

EP - 1136

JO - Environmental Microbiology

JF - Environmental Microbiology

IS - 5

ER -