H19 RNA binds four molecules of insulin-like growth factor II mRNA-binding protein

TY - JOUR

T1 - H19 RNA binds four molecules of insulin-like growth factor II mRNA-binding protein

AU - Runge, Steffen

AU - Nielsen, Finn Cilius

AU - Nielsen, Jacob

AU - Andersen, Jens Lykke

AU - Wewer, Ulla M.

AU - Christiansen, Jan

PY - 2000

Y1 - 2000

N2 - H19 RNA is a major oncofetal 2.5-kilobase untranslated RNA of unknown function. The maternally expressed H19 gene is located 90 kilobase pairs downstream from the paternally expressed insulin-like growth factor II (IGF-II) gene on human chromosome 11 and mouse chromosome 7; and due to their reciprocal imprinting and identical spatiotemporal expression, it is assumed that the two genes are functionally coupled. Here we show that human H19 RNA contains four attachment sites for the oncofetal IGF-II mRNA-binding protein (IMP) with apparent K(d) values in the 0.4-1.3 nm range. The multiple attachment sites are clustered within a 700-nucleotide segment encoded by exons 4 and 5. This 3'-terminal segment targets H19 RNA to lamellipodia and perinuclear regions in dispersed fibroblasts where IMP is also localized. The results suggest that IMP participates in H19 RNA localization and provides a link between the IGF-II and H19 genes at post-transcriptional events during mammalian development.

AB - H19 RNA is a major oncofetal 2.5-kilobase untranslated RNA of unknown function. The maternally expressed H19 gene is located 90 kilobase pairs downstream from the paternally expressed insulin-like growth factor II (IGF-II) gene on human chromosome 11 and mouse chromosome 7; and due to their reciprocal imprinting and identical spatiotemporal expression, it is assumed that the two genes are functionally coupled. Here we show that human H19 RNA contains four attachment sites for the oncofetal IGF-II mRNA-binding protein (IMP) with apparent K(d) values in the 0.4-1.3 nm range. The multiple attachment sites are clustered within a 700-nucleotide segment encoded by exons 4 and 5. This 3'-terminal segment targets H19 RNA to lamellipodia and perinuclear regions in dispersed fibroblasts where IMP is also localized. The results suggest that IMP participates in H19 RNA localization and provides a link between the IGF-II and H19 genes at post-transcriptional events during mammalian development.

KW - Animals

KW - Base Sequence

KW - Binding Sites

KW - Humans

KW - Insulin-Like Growth Factor II

KW - Mice

KW - Molecular Sequence Data

KW - Protein Binding

KW - RNA, Messenger

KW - RNA, Untranslated

KW - RNA-Binding Proteins

U2 - 10.1074/jbc.M001156200

DO - 10.1074/jbc.M001156200

M3 - Journal article

C2 - 10875929

SN - 0021-9258

VL - 275

SP - 29562

EP - 29569

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 38

ER -