Gynecological manifestations, histopathological findings, and schistosoma-specific polymerase chain reaction results among women with Schistosoma haematobium infection: a cross-sectional study in Madagascar

Bodo Sahondra Randrianasolo; Peter Mark Jourdan; Pascaline Ravoniarimbinina; Charles Emile Ramarokoto; Fanjasoa Rakotomanana; Vololomboahangy Elisabeth Ravaoalimalala; Svein Gunnar Gundersen; Hermann Feldmeier; Birgitte J Vennervald; Lisette van Lieshout; Borghild Roald; Peter Leutscher; Eyrun Floerecke Kjetland

doi:10.1093/infdis/jiv035

Gynecological manifestations, histopathological findings, and schistosoma-specific polymerase chain reaction results among women with Schistosoma haematobium infection: a cross-sectional study in Madagascar

Bodo Sahondra Randrianasolo, Peter Mark Jourdan, Pascaline Ravoniarimbinina, Charles Emile Ramarokoto, Fanjasoa Rakotomanana, Vololomboahangy Elisabeth Ravaoalimalala, Svein Gunnar Gundersen, Hermann Feldmeier, Birgitte J Vennervald, Lisette van Lieshout, Borghild Roald, Peter Leutscher, Eyrun Floerecke Kjetland

17 Citationer (Scopus)

Abstract

BACKGROUND: The pathophysiology of female genital schistosomiasis (FGS) is only partially understood. This study aims to describe the histopathological findings, polymerase chain reaction (PCR) results, and gynecological manifestations of FGS in women with different intensities of Schistosoma haematobium infection.

METHODS: Women aged 15-35 years living in an S. haematobium-endemic area in Madagascar underwent pelvic and colposcopic examinations. Small biopsy specimens were obtained from lesions and examined histopathologically. Schistosoma PCR was done on urine, biopsy, cervicovaginal lavage, and genital mucosal surface specimens.

RESULTS: Sandy patches and rubbery papules were found in 41 of 118 women (35%). Rubbery papules reflected an intense cellular immune reaction dominated by eosinophils, epithelial erosion, and viable ova. There was a significant decrease in the prevalence of rubbery papules with age, even after adjustment for urinary ova excretion. The sandy patches with grains showed moderate cellular immune reaction and ova (viable and/or calcified). They were most prevalent in cases with low-intensity urinary S. haematobium infection. Forty-two percent of women with Schistosoma-negative urine specimens had at least 1 genital specimen test positive for Schistosoma by PCR.

CONCLUSIONS: The results indicate a diversity of lesions caused by S. haematobium and a dynamic evolution of the genital lesions. Schistosoma PCR may give an indication of the diagnosis.

Originalsprog	Engelsk
Tidsskrift	The Journal of Infectious Diseases
Vol/bind	212
Udgave nummer	2
Sider (fra-til)	275-284
Antal sider	10
ISSN	0022-1899
DOI	https://doi.org/10.1093/infdis/jiv035
Status	Udgivet - 15 jul. 2015

Emneord

Adolescent
Adult
Animals
Cross-Sectional Studies
Female
Humans
Madagascar
Molecular Diagnostic Techniques
Polymerase Chain Reaction
Schistosoma haematobium
Schistosomiasis haematobia
Uterine Diseases
Young Adult

FN’s Verdensmål

Dette resultat bidrager til følgende verdensmål

Adgang til dokumentet

10.1093/infdis/jiv035

jiv035.pdfForlagets udgivne version, 434 KB

Fingeraftryk

Dyk ned i forskningsemnerne om 'Gynecological manifestations, histopathological findings, and schistosoma-specific polymerase chain reaction results among women with Schistosoma haematobium infection: a cross-sectional study in Madagascar'. Sammen danner de et unikt fingeraftryk.

Citationsformater

Randrianasolo, B. S., Jourdan, P. M., Ravoniarimbinina, P., Ramarokoto, C. E., Rakotomanana, F., Ravaoalimalala, V. E., Gundersen, S. G., Feldmeier, H., Vennervald, B. J., van Lieshout, L., Roald, B., Leutscher, P., & Kjetland, E. F. (2015). Gynecological manifestations, histopathological findings, and schistosoma-specific polymerase chain reaction results among women with Schistosoma haematobium infection: a cross-sectional study in Madagascar. The Journal of Infectious Diseases, 212(2), 275-284. https://doi.org/10.1093/infdis/jiv035

Gynecological manifestations, histopathological findings, and schistosoma-specific polymerase chain reaction results among women with Schistosoma haematobium infection: a cross-sectional study in Madagascar. / Randrianasolo, Bodo Sahondra; Jourdan, Peter Mark; Ravoniarimbinina, Pascaline et al.
I: The Journal of Infectious Diseases, Bind 212, Nr. 2, 15.07.2015, s. 275-284.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Randrianasolo, BS, Jourdan, PM, Ravoniarimbinina, P, Ramarokoto, CE, Rakotomanana, F, Ravaoalimalala, VE, Gundersen, SG, Feldmeier, H, Vennervald, BJ, van Lieshout, L, Roald, B, Leutscher, P & Kjetland, EF 2015, 'Gynecological manifestations, histopathological findings, and schistosoma-specific polymerase chain reaction results among women with Schistosoma haematobium infection: a cross-sectional study in Madagascar', The Journal of Infectious Diseases, bind 212, nr. 2, s. 275-284. https://doi.org/10.1093/infdis/jiv035

Randrianasolo BS, Jourdan PM, Ravoniarimbinina P, Ramarokoto CE, Rakotomanana F, Ravaoalimalala VE et al. Gynecological manifestations, histopathological findings, and schistosoma-specific polymerase chain reaction results among women with Schistosoma haematobium infection: a cross-sectional study in Madagascar. The Journal of Infectious Diseases. 2015 jul. 15;212(2):275-284. doi: 10.1093/infdis/jiv035

Randrianasolo, Bodo Sahondra ; Jourdan, Peter Mark ; Ravoniarimbinina, Pascaline et al. / Gynecological manifestations, histopathological findings, and schistosoma-specific polymerase chain reaction results among women with Schistosoma haematobium infection : a cross-sectional study in Madagascar. I: The Journal of Infectious Diseases. 2015 ; Bind 212, Nr. 2. s. 275-284.

@article{c3b5f598676b4dcebd8f13777dd478c8,

title = "Gynecological manifestations, histopathological findings, and schistosoma-specific polymerase chain reaction results among women with Schistosoma haematobium infection: a cross-sectional study in Madagascar",

abstract = "BACKGROUND: The pathophysiology of female genital schistosomiasis (FGS) is only partially understood. This study aims to describe the histopathological findings, polymerase chain reaction (PCR) results, and gynecological manifestations of FGS in women with different intensities of Schistosoma haematobium infection.METHODS: Women aged 15-35 years living in an S. haematobium-endemic area in Madagascar underwent pelvic and colposcopic examinations. Small biopsy specimens were obtained from lesions and examined histopathologically. Schistosoma PCR was done on urine, biopsy, cervicovaginal lavage, and genital mucosal surface specimens.RESULTS: Sandy patches and rubbery papules were found in 41 of 118 women (35%). Rubbery papules reflected an intense cellular immune reaction dominated by eosinophils, epithelial erosion, and viable ova. There was a significant decrease in the prevalence of rubbery papules with age, even after adjustment for urinary ova excretion. The sandy patches with grains showed moderate cellular immune reaction and ova (viable and/or calcified). They were most prevalent in cases with low-intensity urinary S. haematobium infection. Forty-two percent of women with Schistosoma-negative urine specimens had at least 1 genital specimen test positive for Schistosoma by PCR.CONCLUSIONS: The results indicate a diversity of lesions caused by S. haematobium and a dynamic evolution of the genital lesions. Schistosoma PCR may give an indication of the diagnosis.",

keywords = "Adolescent, Adult, Animals, Cross-Sectional Studies, Female, Humans, Madagascar, Molecular Diagnostic Techniques, Polymerase Chain Reaction, Schistosoma haematobium, Schistosomiasis haematobia, Uterine Diseases, Young Adult",

author = "Randrianasolo, {Bodo Sahondra} and Jourdan, {Peter Mark} and Pascaline Ravoniarimbinina and Ramarokoto, {Charles Emile} and Fanjasoa Rakotomanana and Ravaoalimalala, {Vololomboahangy Elisabeth} and Gundersen, {Svein Gunnar} and Hermann Feldmeier and Vennervald, {Birgitte J} and {van Lieshout}, Lisette and Borghild Roald and Peter Leutscher and Kjetland, {Eyrun Floerecke}",

note = "{\textcopyright} The Author 2015. Published by Oxford University Press on behalf of the Infectious Diseases Society of America.",

year = "2015",

month = jul,

day = "15",

doi = "10.1093/infdis/jiv035",

language = "English",

volume = "212",

pages = "275--284",

journal = "The Journal of Infectious Diseases",

issn = "0022-1899",

publisher = "Oxford University Press",

number = "2",

}

TY - JOUR

T1 - Gynecological manifestations, histopathological findings, and schistosoma-specific polymerase chain reaction results among women with Schistosoma haematobium infection

T2 - a cross-sectional study in Madagascar

AU - Randrianasolo, Bodo Sahondra

AU - Jourdan, Peter Mark

AU - Ravoniarimbinina, Pascaline

AU - Ramarokoto, Charles Emile

AU - Rakotomanana, Fanjasoa

AU - Ravaoalimalala, Vololomboahangy Elisabeth

AU - Gundersen, Svein Gunnar

AU - Feldmeier, Hermann

AU - Vennervald, Birgitte J

AU - van Lieshout, Lisette

AU - Roald, Borghild

AU - Leutscher, Peter

AU - Kjetland, Eyrun Floerecke

N1 - © The Author 2015. Published by Oxford University Press on behalf of the Infectious Diseases Society of America.

PY - 2015/7/15

Y1 - 2015/7/15

N2 - BACKGROUND: The pathophysiology of female genital schistosomiasis (FGS) is only partially understood. This study aims to describe the histopathological findings, polymerase chain reaction (PCR) results, and gynecological manifestations of FGS in women with different intensities of Schistosoma haematobium infection.METHODS: Women aged 15-35 years living in an S. haematobium-endemic area in Madagascar underwent pelvic and colposcopic examinations. Small biopsy specimens were obtained from lesions and examined histopathologically. Schistosoma PCR was done on urine, biopsy, cervicovaginal lavage, and genital mucosal surface specimens.RESULTS: Sandy patches and rubbery papules were found in 41 of 118 women (35%). Rubbery papules reflected an intense cellular immune reaction dominated by eosinophils, epithelial erosion, and viable ova. There was a significant decrease in the prevalence of rubbery papules with age, even after adjustment for urinary ova excretion. The sandy patches with grains showed moderate cellular immune reaction and ova (viable and/or calcified). They were most prevalent in cases with low-intensity urinary S. haematobium infection. Forty-two percent of women with Schistosoma-negative urine specimens had at least 1 genital specimen test positive for Schistosoma by PCR.CONCLUSIONS: The results indicate a diversity of lesions caused by S. haematobium and a dynamic evolution of the genital lesions. Schistosoma PCR may give an indication of the diagnosis.

AB - BACKGROUND: The pathophysiology of female genital schistosomiasis (FGS) is only partially understood. This study aims to describe the histopathological findings, polymerase chain reaction (PCR) results, and gynecological manifestations of FGS in women with different intensities of Schistosoma haematobium infection.METHODS: Women aged 15-35 years living in an S. haematobium-endemic area in Madagascar underwent pelvic and colposcopic examinations. Small biopsy specimens were obtained from lesions and examined histopathologically. Schistosoma PCR was done on urine, biopsy, cervicovaginal lavage, and genital mucosal surface specimens.RESULTS: Sandy patches and rubbery papules were found in 41 of 118 women (35%). Rubbery papules reflected an intense cellular immune reaction dominated by eosinophils, epithelial erosion, and viable ova. There was a significant decrease in the prevalence of rubbery papules with age, even after adjustment for urinary ova excretion. The sandy patches with grains showed moderate cellular immune reaction and ova (viable and/or calcified). They were most prevalent in cases with low-intensity urinary S. haematobium infection. Forty-two percent of women with Schistosoma-negative urine specimens had at least 1 genital specimen test positive for Schistosoma by PCR.CONCLUSIONS: The results indicate a diversity of lesions caused by S. haematobium and a dynamic evolution of the genital lesions. Schistosoma PCR may give an indication of the diagnosis.

KW - Adolescent

KW - Adult

KW - Animals

KW - Cross-Sectional Studies

KW - Female

KW - Humans

KW - Madagascar

KW - Molecular Diagnostic Techniques

KW - Polymerase Chain Reaction

KW - Schistosoma haematobium

KW - Schistosomiasis haematobia

KW - Uterine Diseases

KW - Young Adult

U2 - 10.1093/infdis/jiv035

DO - 10.1093/infdis/jiv035

M3 - Journal article

C2 - 25725656

SN - 0022-1899

VL - 212

SP - 275

EP - 284

JO - The Journal of Infectious Diseases

JF - The Journal of Infectious Diseases

IS - 2

ER -